Figure 1. The central role of pDCs and the produced IFN-I in promoting inflammatory and autoimmune diseases. IFN-I and IFN-III are produced by pDCs in response to signals delivered by TLR7 and/or TLR9 in response to the sensing of self–nucleic More about this image found in The central role of pDCs and the produced IFN-I in promoting inflammatory a...

Figure 2. CD4-mediated endocytosis of HIV-1 leads to persistent/repeated IFN-I induction in pDCs. HIV-1/CD4 traffics predominantly to early and recycling endosomes, where HIV-1 genomic RNA interacts with TLR7 to activate MYD88, NFκB, IRF7, and More about this image found in CD4-mediated endocytosis of HIV-1 leads to persistent/repeated IFN-I induct...

Figure 3. Induction of inflammatory or immature pDCs (ipDCs) during chronic HIV-1 infection. HIV-1 activates pDCs via CD4-dependent endocytosis and HIV-1 RNA-mediated TLR7 activation (1). Persistent activation of pDCs by HIV-1 leads to More about this image found in Induction of inflammatory or immature pDCs (ipDCs) during chronic HIV-1 inf...

Figure 1. Identification of IL-10–producing CD25⁺ FOXP3⁻ human T_F cells in human tonsils. (a) Flow cytometric plots showing gating strategy to identify the indicated populations. (b) Flow cytometric plots and quantification (n = 8) showing More about this image found in Identification of IL-10–producing CD25⁺ FOXP3⁻ human ...

Figure 2. Human CD25⁺ T_F cells repress T cell proliferation. (a) Volcano plot showing Affymetrix RNA microarrays comparing gene expression between IL-10–producing and nonproducing T_F cells (n = 3). (b) Flow cytometric plots showing More about this image found in Human CD25⁺ T_F cells repress T cell proliferation. (a...

Figure 3. Human CD25⁺ T_F cells may be peripherally induced and express high CTLA4 but low IL-2 production. (a) Bisulfite sequencing of the 11 CpG islands of the FOXP3 CNS2 locus with 12 representative clones per population and per donor (filled More about this image found in Human CD25⁺ T_F cells may be peripherally induced and ...

Figure 4. Human CD25⁺ T_F cells’ transcriptome resembles that of murine T_FR cells. (a) Flow cytometric plots showing the gating strategy used to FACS-purify each of the indicated cell subsets. (b) Heatmap analysis of RNA-seq data showing More about this image found in Human CD25⁺ T_F cells’ transcriptome resembles that of...

Figure 5. Human CD25⁺ T_F cells repress T_FH cells and induction of IgE switching. (a and b) Flow cytometric plots (a) and quantification (n = 8; b) of CTV-labeled-T_FH cells, cocultured with memory B cells with or without CD25⁺ T_F cells, showing More about this image found in Human CD25⁺ T_F cells repress T_FH cells and...

Figure 6. Tonsillar CD25⁺ T_F cells inversely correlate to total IgE in the serum. (a and b) Pearson correlation analyses between serum total IgE and the frequency of the indicated cell subset in tonsil (n = 49). Data are representative of two More about this image found in Tonsillar CD25⁺ T_F cells inversely correlate to total...

Figure 1. Increased lupus nephritis severity in P2rx7^−/− mice. (A) Spleen weight of untreated WT (n = 7), P2rx7^−/− (n = 6), pristane-treated WT (n = 19), and P2rx7^−/− (n = 18) mice. (B) Proteinuria score of pristane-treated WT and P2rx7^−/− More about this image found in Increased lupus nephritis severity in P2rx7^−/− ...

Figure 2. Increased serum autoantibodies and splenic plasma cells in P2rx7^−/− mice. (A) Serum Ig isotype concentrations in untreated (n = 6 for IgM and IgA, n = 16 for total IgG, n = 12 for IgG subclasses) and pristane-treated (n = 15 for IgM More about this image found in Increased serum autoantibodies and splenic plasma cells in P2rx7...

Figure 3. Increased GC reaction and Tfh cells in P2rx7^−/− mice. (A) Representative contour plots for PNA and Fas staining on gated CD19⁺B220⁺ splenocytes, and frequency and absolute number in untreated (n = 12) and treated (n = 37) WT as well as More about this image found in Increased GC reaction and Tfh cells in P2rx7^−/−...

Figure 4. Impaired primary, secondary, and memory responses to NP-OVA in P2rx7^−/− mice. (A) Scheme of the immunization protocol and time points of analysis. (B) Frequency and absolute number of CXCR5⁺ICOS⁺ Tfh cells in the draining lymph nodes More about this image found in Impaired primary, secondary, and memory responses to NP-OVA in P2rx...

Figure 5. Selective expansion of P2rx7^−/− Tfh cells upon pristane injection but not conventional immunization . (A) Scheme of the adoptive transfer and immunization experiment; representative plots of donor CD4⁺ and CXCR5⁺ICOS⁺ T cells within More about this image found in Selective expansion of P2rx7^−/− Tfh cells upon ...

Figure 6. P2X7-mediated control of PSGL-1 down-regulation and IFN-γ secretion in pristane-treated mice . (A) Frequency of PNA⁺Fas⁺ GC splenic B cells (untreated WT, n = 3; P2rx7^−/−, n = 3; and Icos^−/−P2rx7^−/−, n = 3; pristane-treated WT, n = 9; More about this image found in P2X7-mediated control of PSGL-1 down-regulation and IFN-γ secretion in pris...

Figure 7. Increased PIL severity and IFN-γ secretion by ICOS⁺PSGL-1^lo/− CD4 T cells in mice with conditional deletion of P2rx7 in T cells. (A) Spleen weight of untreated CD4-Cre P2rx7^WT/WT (n = 5), CD4-Cre P2rx7^fl/fl (n = 12), pristane-treated More about this image found in Increased PIL severity and IFN-γ secretion by ICOS⁺PSGL-1^lo...

Figure 8. P2X7-mediated caspase activation and Gsdmd cleavage in Tfh cells. (A) Time monitoring of YO-PRO-1 uptake after stimulation with BzATP in ICOS⁻PD-1⁻ and ICOS⁺PD-1⁺ cells within CXCR5⁺ CD4⁺ T cells from spleen of WT and P2rx7^−/− mice at More about this image found in P2X7-mediated caspase activation and Gsdmd cleavage in Tfh cells. (A) Time...