Issues

Localization of distinct antigen-experienced CD8⁺ T cell populations in secondary lymphoid tissues. The figure shows four three-color confocal micrographs of cryostat sections of spleens (top row) and peripheral lymph nodes (bottom row) from recipient mice 24 h after intravenous injection of tetramethylrhodamine isothiocyanate (TRITC)-labeled cytotoxic effector cells (left column) or central memory cells (right column). The sections were counterstained with anti-B220 (green) and anti­Thy-1.2 (blue) to localize B cell follicles and T cell areas, respectively. TRITC-labeled homed donor cells are identified by red fluorescence. For details on differential in vivo trafficking of effector and central memory cells see related article in this issue by Weninger et al., pp. 953-966.