To study the role of CD8+ T cells in allergic sensitization, we examined the effects of in vivo depletion of CD8+ T cells prior to sensitization on IgE production, immediate type cutaneous hypersensitivity and development of altered airway responsiveness. BALB/c mice were thymectomized and treated with anti-CD8 antibody resulting in depletion of CD8+ T cells (<1%) in spleen and lymphoid tissues. In these mice, sensitization to ovalbumin (OVA) via the airways still resulted in IgE anti-OVA responses and immediate cutaneous reactions to OVA, but the animals were unable to develop airway hyperresponsiveness, eosinophil infiltration of the lung parenchyma, or IL-5 production in the local lymph nodes of the airway. Transfer of CD8+ T cells from naive animals during sensitization (on day 8 of the 10-d protocol) fully restored the ability to develop airway hyperresponsiveness and this was accompanied by IL-5 production and eosinophil accumulation in the lung. These data indicate a critical role for CD8+ T cells in the production of IL-5 and the development of altered airway responsiveness after antigen sensitization through the airways.
Requirement for CD8+ T cells in the development of airway hyperresponsiveness in a marine model of airway sensitization.
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E Hamelmann, A Oshiba, J Paluh, K Bradley, J Loader, T A Potter, G L Larsen, E W Gelfand; Requirement for CD8+ T cells in the development of airway hyperresponsiveness in a marine model of airway sensitization.. J Exp Med 1 April 1996; 183 (4): 1719–1729. doi: https://doi.org/10.1084/jem.183.4.1719
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