In the current study, we address the underlying mechanism for the selective generation of gut-homing T cells in the gut-associated lymphoid tissues (GALT). We demonstrate that DCs in the GALT are unique in their capacity to establish T cell gut tropism but in vivo only confer this property to T cells in the presence of DC maturational stimuli, including toll-like receptor-dependent and -independent adjuvants. Thus, DCs from mesenteric LNs (MLNs), but not from spleen, supported expression of the chemokine receptor CCR9 and integrin α4β7 by activated CD8+ T cells. While DCs were also required for an efficient down-regulation of CD62L, this function was not restricted to MLN DCs. In an adoptive CD8+ T cell transfer model, antigen-specific T cells entering the small intestinal epithelium were homogeneously CCR9+α4β7+CD62Llow, and this phenotype was only generated in GALT and in the presence of adjuvant. Consistent with the CCR9+ phenotype of the gut-homing T cells, CCR9 was found to play a critical role in the localization of T cells to the small intestinal epithelium. Together, these results demonstrate that GALT DCs and T cell expression of CCR9 play critical and integrated roles during T cell homing to the gut.
Skip Nav Destination
Article navigation
15 September 2003
Brief Definitive Report|
September 08 2003
Selective Generation of Gut Tropic T Cells in Gut-associated Lymphoid Tissue (GALT) : Requirement for GALT Dendritic Cells and Adjuvant
Bengt Johansson-Lindbom,
Bengt Johansson-Lindbom
1Immunology Section, Department of Cell and Molecular Biology, Lund University, BMC I-13, S-22184 Lund, Sweden
Search for other works by this author on:
Marcus Svensson,
Marcus Svensson
1Immunology Section, Department of Cell and Molecular Biology, Lund University, BMC I-13, S-22184 Lund, Sweden
Search for other works by this author on:
Marc-André Wurbel,
Marc-André Wurbel
2Centre d'Imunnologie de Marseille-Luminy, Institut National de la Santé et de la Recherche Médicale–Centre National de la Recherche Scientifique–Université de la Méditerranée, Case 906, 13288 Marseille Cedex 9, France
Search for other works by this author on:
Bernard Malissen,
Bernard Malissen
2Centre d'Imunnologie de Marseille-Luminy, Institut National de la Santé et de la Recherche Médicale–Centre National de la Recherche Scientifique–Université de la Méditerranée, Case 906, 13288 Marseille Cedex 9, France
Search for other works by this author on:
Gabriel Márquez,
Gabriel Márquez
3Departamento de Inmunologia y Oncologia, Centro Nacional de Biotecnologia/Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Cantoblanco, 28040-Madrid, Spain
Search for other works by this author on:
William Agace
William Agace
1Immunology Section, Department of Cell and Molecular Biology, Lund University, BMC I-13, S-22184 Lund, Sweden
Search for other works by this author on:
Bengt Johansson-Lindbom
1Immunology Section, Department of Cell and Molecular Biology, Lund University, BMC I-13, S-22184 Lund, Sweden
Marcus Svensson
1Immunology Section, Department of Cell and Molecular Biology, Lund University, BMC I-13, S-22184 Lund, Sweden
Marc-André Wurbel
2Centre d'Imunnologie de Marseille-Luminy, Institut National de la Santé et de la Recherche Médicale–Centre National de la Recherche Scientifique–Université de la Méditerranée, Case 906, 13288 Marseille Cedex 9, France
Bernard Malissen
2Centre d'Imunnologie de Marseille-Luminy, Institut National de la Santé et de la Recherche Médicale–Centre National de la Recherche Scientifique–Université de la Méditerranée, Case 906, 13288 Marseille Cedex 9, France
Gabriel Márquez
3Departamento de Inmunologia y Oncologia, Centro Nacional de Biotecnologia/Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Cantoblanco, 28040-Madrid, Spain
William Agace
1Immunology Section, Department of Cell and Molecular Biology, Lund University, BMC I-13, S-22184 Lund, Sweden
Address correspondence to Bengt Johansson-Lindbom, Immunology Section, Dept. of Cell and Molecular Biology, Lund University, BMC I-13, S-22184 Lund, Sweden. Phone: 46-46-2220313; Fax: 46-46-2224218; email: [email protected]
B. Johannsson-Lindbom and M. Svensson contributed equally to this work.
Received:
July 24 2003
Revision Received:
August 08 2003
Accepted:
August 08 2003
Online ISSN: 1540-9538
Print ISSN: 0022-1007
The Rockefeller University Press
2003
J Exp Med (2003) 198 (6): 963–969.
Article history
Received:
July 24 2003
Revision Received:
August 08 2003
Accepted:
August 08 2003
Citation
Bengt Johansson-Lindbom, Marcus Svensson, Marc-André Wurbel, Bernard Malissen, Gabriel Márquez, William Agace; Selective Generation of Gut Tropic T Cells in Gut-associated Lymphoid Tissue (GALT) : Requirement for GALT Dendritic Cells and Adjuvant . J Exp Med 15 September 2003; 198 (6): 963–969. doi: https://doi.org/10.1084/jem.20031244
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionSuggested Content
Email alerts
Advertisement