Central memory CD8+ T cells (TCM) confer superior protective immunity against infections compared with other T cell subsets. TCM recirculate mainly through secondary lymphoid organs, including peripheral lymph nodes (PLNs). Here, we report that TCM, unlike naive T cells, can home to PLNs in both a CCR7-dependent and -independent manner. Homing experiments in paucity of lymph node T cells (plt/plt) mice, which do not express CCR7 ligands in secondary lymphoid organs, revealed that TCM migrate to PLNs at ∼20% of wild-type (WT) levels, whereas homing of naive T cells was reduced by 95%. Accordingly, a large fraction of endogenous CD8+ T cells in plt/plt PLNs displayed a TCM phenotype. Intravital microscopy of plt/plt subiliac lymph nodes showed that TCM rolled and firmly adhered (sticking) in high endothelial venules (HEVs), whereas naive T cells were incapable of sticking. Sticking of TCM in plt/plt HEVs was pertussis toxin sensitive and was blocked by anti-CXCL12 (SDF-1α). Anti-CXCL12 also reduced homing of TCM to PLNs in WT animals by 20%, indicating a nonredundant role for this chemokine in the presence of physiologic CCR7 agonists. Together, these data distinguish naive T cells from TCM, whereby only the latter display greater migratory flexibility by virtue of their increased responsiveness to both CCR7 ligands and CXCL12 during homing to PLN.


Upon Ag encounter, naive T cells differentiate into effector cells poised to eradicate foreign or tumor Ags. Upon completing this task, most effector T cells die, leaving behind a small population of memory T cells that respond more rapidly to reencountered Ags (13). Based on the expression of certain adhesion molecules and chemokine receptors, two distinct subsets of memory T cells have been shown to arise during immune responses as follows: central memory T cells (TCM) express L-selectin and CCR7 and localize to secondary lymphoid organs, whereas effector memory T cells (TEM) are L-selectinCCR7 and found mainly in nonlymphoid tissues (4). When isolated from human blood, CD8+ TEM show greater cytotoxicity and produce more effector cytokines than TCM. However, adoptive transfer of CD8+ TCM into naive recipients is more efficient than transfer of TEM in conferring protective immunity. This may be due to the higher proliferative potential of TCM as well as their greater capacity to persist in vivo (5). CD8+ TEM can differentiate into TCM over several weeks; i.e., they reexpress L-selectin and CCR7 and acquire an enhanced capacity of homeostatic and Ag-driven proliferation (5). In addition, naive T cells can give rise to TCM without first experiencing a full-fledged effector phase (6).

The localization of TCM in LNs might further contribute to their key role in immune protection. Professional APCs, in particular DCs, transport Ag from the periphery into the T cell zones of the LN (7). Thus, TCM are strategically positioned to interact with DCs, which provide optimal stimulatory signals if TCM detect a recall Ag on their surface.

We have recently described a method that allows for the generation of central memory–like CD8+ T cells in vitro (6). Ag-primed CD8+ T cells cultured in IL-15 for 5–7 d express high levels of CD44, L-selectin, and CCR7. Upon TCR stimulation, they produce IFN-γ, but are not acutely cytotoxic in vitro. After adoptive transfer, these cells survive for long periods of time, and mount rapid Ag-specific recall responses. We have shown that these cells migrate to all secondary lymphoid organs, including peripheral LNs (PLNs), mesenteric LNs (MLNs), Peyer's patches, and spleen (8). Similar to naive T cells, migration to PLNs occurred via high endothelial venules (HEVs) and depended on L-selectin (8).

To address the role of CCR7 in PLN homing, TCM were injected into paucity of lymph node T cells (plt/plt) mice in which the lymphoid organ–expressed CCR7 ligands CCL19 (ELC) and CCL21-Ser (SLC) are deleted (912). The few T cells present in plt/plt PLNs are enriched for memory cells (10), shown here to be predominantly TCM, indicating that this subset can home to LNs in the absence of CCR7 ligands. Indeed, although naive T cell homing was reduced to 5%, TCM migration to plt/plt PLNs was reduced to only 20% of WT levels (8). This suggests involvement of one or more CCR7-independent pathways that enable at least some TCM to home to PLNs.

In this paper, we show that TCM rolled and adhered firmly in plt/plt PLN HEVs. TCM sticking and migration to plt/plt PLNs were pertussis toxin (PTX) sensitive and CXCL12 dependent. Homing of TCM to WT PLNs was also partially dependent on CXCL12. In contrast, CXCL12 had no detectable effect on naive T cell trafficking to PLNs. Thus, TCM use at least two different chemokine pathways, CCR7–CCL19/21 and CXCR4–CXCL12, to enter PLNs under steady state conditions.

Materials And Methods


DDD/1-plt/plt, BALB/c-plt/plt, and DDD1-mtv/mtv control mice were provided by A. Matsuzawa (University of Tokyo, Tokyo, Japan). Transgenic T–green fluorescent protein (GFP) mice were generated in our laboratory (13). CXCR3−/− mice were provided by C. Gerard (Harvard University, Boston, MA). C57BL/6 and BALB/c mice were purchased from The Jackson Laboratory. Mice were housed and bred in a specific pathogen-free and viral antibody-free animal facility. Experiments were in accordance with National Institutes of Health guidelines and approved by the Committees on Animals of Harvard Medical School and The CBR Institute for Biomedical Research.


Fluorochrome-labeled mAbs were obtained from BD Biosciences as follows: CD3ε, CD4, CD8α, CD44, CD25, L-selectin, CD122, CD69, and TCRβ. To detect CCR7 and P-selectin ligand expression, recombinant human CCL19-Ig and P-selectin–Ig chimeras were used (6). Antimurine CXCL12, recombinant murine CXCL12, CCL2, CCL5, CCL19, and recombinant human IL-15 were obtained from R&D Systems. PTX was obtained from Calbiochem.

In Vitro Differentiation of TCM

TCM were generated as described previously (6). In brief, splenocytes were incubated with 1 μg/ml anti-CD3ε and 2 d later with media containing 20 ng/ml IL-15 (for 5–8 d). Before each experiment, activation marker and homing molecule expression were assessed by flow cytometry (8).

ELISA and Immunofluorescence

PLNs were removed from WT and plt/plt mice, homogenized in lysis buffer (radioimmunoprecipitation assay buffer with 1 mM PMSF, 10 μg/ml aprotinin, and 10 μg/ml leupeptin), and centrifuged (14k g at 4°C for 10 min). The supernatant was assayed for CXCL12 immunoreactivity by ELISA (R&D Systems). Immunostaining of frozen sections was performed as described previously (14).

Homing Assays

Homing assays were performed as described previously (8). In brief, tetramethylrhodamine-5-isothiocyanate (TRITC)-labeled TCM were mixed with LN cells from T-GFP mice and injected i.v. into recipients. After 1 or 24 h, 1 ml PBLs, spleen, PLNs, and MLNs were harvested, immunostained, and analyzed by flow cytometry. The homing index in organs was calculated as the ratio between the number of CD8+TRITC+ (TCM) and CD8+GFP+ (naive) T cells divided by the ratio of CD8+TRITC+ and CD8+GFP+ cells in the input (8). In some experiments, TCM were pretreated with 100 ng/ml PTX for 2 h before adoptive transfer. For blocking experiments, 100 μg/mouse anti-CXCL12 or control mAb were injected i.v. 15 min before adoptive transfer of T cells.

Intravital Microscopy and Image Analysis

Subiliac LN Preparation.

Surgical preparation of LNs was performed as described previously (15). TCM were labeled with calcein (Molecular Probes), and small boli of cells were injected intraarterially. T cell–endothelial cell interactions in subiliac LNs downstream from the injection site were recorded. For experiments testing the role of CXCL12, cell behavior was analyzed in the same vessels before and after mAb injection. To assess the role of Gαi-protein coupled receptors (GPCRs), PTX-treated and untreated TCM were compared.

Cremaster Muscle Preparation.

Cremaster muscles of C57BL/6 mice were prepared as described previously (16). Calcein-labeled TCM were injected, and several postcapillary and small collecting venules were recorded during a 15-min control period to determine baseline rolling and sticking. Subsequently, superfusion buffer was replaced with buffer containing 100 nM CXCL12, and TCM behavior was recorded in the same vessels for an additional 15 min.

Offline frame-by-frame video analysis was performed as described previously (17). Rolling fraction was determined as the percentage of cells interacting with HEVs in the total number of cells passing through a vessel during the observation period. Sticking fraction was defined as the percentage of rolling cells that adhered in HEVs for ≥30 s.

Statistical Analysis.

All data are presented as mean ± SEM. Homing indices were compared using the unpaired Student's t test. Rolling and sticking fractions were compared using the paired Student's t test.

Results And Discussion

Preferential Recruitment of TCM to plt/plt PLNs.

T cells can enter LNs from the blood or via afferent lymphatic vessels draining tissues such as the skin (18). From the blood, naive T cells must adhere to HEVs, a process requiring L-selectin, CCR7, LFA-1, and their respective ligands (14, 18, 19). Similar to naive T cells, TCM home to PLNs via HEVs in an L-selectin– and partially CCR7-dependent manner (8, 14, 19). Although naive T cells do not arrest in HEVs and home very poorly to plt/plt PLNs (14), TCM are capable of entering these organs via HEVs, albeit considerably less efficiently than in WT mice (8). Competitive homing of naive T cells and TCM in DDD/1-plt/plt mice revealed that the total number of homed naive T cells was reduced 19.2-fold compared with WT mice, whereas TCM were only reduced by 4.9-fold (8). Recent observations suggest the defect in T cell migration to plt/plt LNs can vary with the genetic background (20). Thus, we repeated competitive homing assays in plt/plt mice on both BALB/c and DDD/1 backgrounds. In accordance with previous results (8), homing indices in BALB/c and DDD/1-mtv/mtv mice were 0.6 ± 0.1 and 0.4 ± 0.01, respectively; i.e., naive T cells accumulated approximately twofold more efficiently in WT PLNs than TCM (Fig. 1, A and B)

. In contrast, homing indices were 1.4 ± 0.1 and 1.4 ± 0.2 in BALB/c-plt/plt and DDD/1-plt/plt PLN, respectively, indicating the CCR7 pathway may not be absolutely required for TCM homing to PLNs. Both TCM and naive T cells were equally affected in homing to MLNs in both DDD/1-plt/plt mice (by 89 and 88%, respectively) and BALB/c-plt/plt mice (by 92 and 94%, respectively). Thus, CCR7 is indispensable for TCM trafficking to MLNs, whereas PLNs can recruit at least some TCM in the absence of CCL19 and CCL21.

PLN in plt/plt mice are smaller than in WT animals, and contain a larger fraction of Ag-experienced lymphocytes (9, 10). However, the exact composition of memory T cells in these organs has not been determined. Because in vitro–generated TCM retained, in part, the capacity to home to plt/plt PLNs, we examined the phenotype of resident CD8+ memory T cells by staining BALB/c-plt/plt or BALB/c PLN single cell suspensions for CD122 (21). Expression of this memory marker correlated well with that of CD44 (Fig. 1 C). There was an approximately fivefold increase in the percentage of CD8+CD122+ T cells in plt/plt PLNs as compared with WT PLNs. Almost all of them expressed L-selectin and bound CCL19-Ig chimera, but little or no P-selectin–Ig, thus displaying a central memory phenotype (4). These observations strongly suggest that, in contrast to naive T cells, which depend almost entirely on CCR7 ligands, there may be a second homing pathway in PLNs that allows TCM recruitment in the absence of CCL21 and CCL19.

TCM Roll and Stick in plt/plt HEVs.

There are two nonexclusive explanations for preferential TCM traffic to plt/plt PLNs as follows: TCM may have the unique ability to interact with HEVs by responding to an integrin-activating signal distinct from CCR7 ligands and/or they may enter via afferent lymphatic vessels. The latter mechanism was proposed by Mori et al., who observed delayed, but enhanced and prolonged T cell–mediated immune responses to contact sensitizers in plt/plt PLNs (22). Due to CCL21-leu expression in lymphatic vessels in plt/plt mice, both T cells and DCs can enter these vessels to migrate to PLNs (11, 22, 23). However, we show here that several TCM accumulated in plt/plt PLNs as early as 1 h after i.v. injection. Given this brief interval, it seems unlikely that the homed cells could migrate to peripheral tissues and find their way into afferent lymphatics and from there into the cortex of draining LNs. Thus, our findings strongly suggest that TCM can enter plt/plt PLN from the blood.

To test this hypothesis, we used intravital microscopy (IVM) to analyze TCM behavior in the subiliac LN microcirculation of plt/plt mice. The venular tree of normal subiliac LNs consists of five successive venular branching orders, distinguishable by IVM (15). Order V represents postcapillary venules in the cortex and order I is the large collecting vessel at the LN hilus. In WT mice, naive T cells and TCM roll and stick mainly in order III–V venules. These venules express the most peripheral node addressin, which mediates the rolling of L-selectin+ T cells (8, 18, 19, 24). Firm adhesion requires activation of the integrin LFA-1 on rolling lymphocytes, which is usually induced by CCR7 ligands displayed in HEVs (14).

plt/plt PLNs are smaller than WT PLNs and their microarchitecture is disorganized, but the venular tree is readily discernible by IVM in DDD/1-plt/plt PLNs, even though order V venules are typically absent (14). By contrast, BALB/c-plt/plt PLNs are not amenable to IVM because the rarefied venular tree in this strain is obscured by overlying B cell follicles (14). Calcein-labeled TCM were recorded in 43 HEVs (7 order I, 12 order II, 16 order III, and 8 order IV) in 6 DDD1-plt/plt mice (Fig. 2)

. Mean rolling fractions of TCM were 17 ± 5% (order I), 23 ± 3% (order II), 40 ± 5% (order III), and 33 ± 7% (order IV). This is comparable to rolling fractions of TCM in WT PLNs (8). For comparison, naive GFP+ T cells (14) were analyzed in 29 venules (2, order I; 8, order II; 8, order III; and 11, order IV) in seven mice. Their mean rolling fractions were 7 ± 2% (order I), 35 ± 9% (order II), 58 ± 4% (order III), and 59 ± 6% (order IV). Naive T cells rolled at similar frequencies in PLN HEVs of DDD/1-plt/plt and DDD/1-mtv/mtv mice, but completely failed to arrest in the former, indicating that CCR7 ligands are absolutely required (Fig. 2 and reference 14). However, TCM were unmistakably capable of sticking in DDD/1-plt/plt PLN HEVs (Fig. 2 B, mean sticking fractions: order I, 2.5 ± 1.8%; order II, 11.5 ± 3.3%; order III, 21.0 ± 4.4%; and order IV, 31.5 ± 1.8%). We conclude that integrins are efficiently activated on rolling TCM in plt/plt PLNs, indicating that an alternative chemoattractant pathway can be used by these cells. Moreover, HEVs are the likely port of PLN entry for TCM in plt/plt mice.

Homing of TCM to plt/plt PLN Is PTX Sensitive.

Chemokine receptors are GPCRs whose function is efficiently blocked by PTX (25, 26). To determine whether CCR7-independent integrin activation signals on TCM were perceived through such GPCRs, TCM were preincubated with PTX before in vivo testing. PTX did not affect cell viability, but inhibited in vitro chemotaxis of TCM toward CCL21 and CXCL12 by 80% (unpublished data). PTX treatment greatly reduced the ability of TCM to home to BALB/c-plt/plt PLNs and MLNs, but had no effect on the number of TCM in PBLs or spleen (Fig. 3, A and B)

. These results were further substantiated by IVM of DDD/1-plt/plt PLNs. There was no difference in the rolling fractions of PTX-treated and untreated TCM (Fig. 3 C, mean: 22 ± 2% and 28 ± 3%, respectively; P > 0.05). This is not surprising because L-selectin–mediated rolling is insensitive to PTX (19). In contrast, the sticking fraction of control TCM was fivefold higher than that of PTX-treated cells (Fig. 3 D, mean: 15.1 ± 3.4% and 2.8 ± 1.6%, respectively; P < 0.01). This indicates that one or more GPCRs, other than CCR7, mediates rapid integrin activation on rolling TCM in PLN HEVs of plt/plt mice.

Homing of TCM to DDD/1-plt/plt PLNs Is Partially Mediated by CXCL12.

One chemoattractant candidate that could mediate TCM homing to PLNs in the absence of CCR7 ligands is CXCL12. mRNA for CXCL12 is expressed by stromal cells in close vicinity to PLN HEVs, and CXCL12 protein was found in the lumen of some cortical HEVs (20). Moreover, CXCL12 and its receptor CXCR4 partly mediate homing of B cells to LN via HEV (20). In the case of T cell homing to plt/plt PLNs, the role of CXCR4–CXCL12 is more complex. Okada et al. found that CXCR4−/− T cells have no discernible defect in homing to WT PLNs, but CXCR4−/− splenic T cells were more severely compromised than WT T cells in their ability to migrate to plt/plt PLNs (20). Notably, the homing experiments with CXCR4−/− T cells were performed with total splenocytes in which the T cell population typically contains ∼30% memory cells. Conceivably, CXCL12-responsive T cells recovered from plt/plt PLNs by Okada et al. may have contained a disproportionate fraction of TCM, rather than naive T cells, which predominate in WT PLNs.

Normal expression of CXCL12 in plt/plt PLNs makes this a good candidate for CCR7-independent TCM migration to PLNs. Thus, we performed IVM of the subiliac LNs in DDD/1-plt/plt mice before and after anti-CXCL12 treatment. A total of 22 HEVs of orders III and IV in three DDD/1-plt/plt recipients were analyzed. Rolling fractions did not change after injection of anti-CXCL12 (Fig. 4 

A, before: 25.0 ± 3.7%; after: 30.7 ± 5.0%; P > 0.05). In contrast, anti-CXCL12 markedly reduced the sticking fraction of TCM from 11.9 ± 2.8% to 2.6 ± 1.2% (Fig. 4 B, P < 0.01).

To determine whether CXCL12 mediates TCM homing to plt/plt PLNs, anti-CXCL12 mAb was injected i.v. into DDD/1-plt/plt mice 15 min before injection of fluorescently tagged TCM. Anti-CXCL12 had no effect on the accumulation of TCM in PBLs, spleen, or MLNs after 1 h (Fig. 4, C and D). In contrast, the number of TCM that homed to PLNs was ∼30% lower than in control mice (Fig. 4 D, P < 0.05). We also performed competitive homing experiments using naive GFP+ T cells and TRITC-labeled TCM in DDD/1-plt/plt mice after anti-CXCL12 or control mAb injection (n = 2). Anti-CXCL12 did not alter the low number of naive T cells that homed to PLNs (unpublished data). However, consistent with the aforementioned data, the homing index decreased from 1.4 ± 0.1 to 1.0 ± 0.2, indicating that TCM, but not naive T cells, home to PLNs in a partially CXCL12-dependent fashion. In contrast with DDD/1-plt/plt mice, anti-CXCL12 had no significant effect on TCM homing to BALB/c-plt/plt PLNs (Fig. 4 C). As a possible explanation for this apparent discrepancy, we asked whether PLN HEVs in BALB/c-plt/plt mice present less CXCL12 on their luminal surface than their counterparts in DDD/1-plt/plt. However, ELISA of PLN lysates and immunostaining of frozen PLN sections from both strains did not reveal detectable differences in CXCL12 protein content and expression pattern, at least at the light microscopic level (unpublished data). Therefore, it is more likely that the lack of CXCL12 contribution to TCM homing in BALB/c-plt/plt mice occurred because the venular tree in PLNs of this strain is very poorly developed compared with that in DDD/1-plt/plt (14). The available HEV surface area in the former may simply be too small to permit effective TCM recruitment, despite CXCL12 expression. This explanation is consistent with the finding that the absolute number of TCM that homed to BALB/c-plt/plt PLNs was three times lower than that recovered from DDD/1-plt/plt PLNs (85 ± 20 homed TCM/106 injected cells versus 267 ± 116/106 injected cells, respectively; P = 0.05; Fig. 4, C and D, and not depicted).

Because our competitive homing experiments indicate that some TCM home to BALB/c-plt/plt PLNs in a CCR7-independent fashion (Fig. 1 A), it is likely that one or more other chemoattractants may be expressed in PLN HEVs of BALB/c-plt/plt mice that mediate TCM homing. The existence of additional GPCR-dependent recruitment signals appears likely, even in DDD/1 mice because PTX treatment blocked TCM homing to PLNs in both plt/plt strains more completely than anti-CXCL12 (Fig. 3 B). Because in vitro–generated TCM respond to CCL2 (monocyte chemoattractant protein–1) and CCL5 (regulated on activation, normal T cell expressed, and secreted [RANTES]; reference 8), we tested whether these chemokines contribute to TCM homing to BALB/c-plt/plt PLNs. Furthermore, very low levels of CCL21-leu mRNA have been detected in plt/plt mice (27). Therefore, we desensitized TCM with a 40-min incubation in 1 μM CCL2, CCL5, or CCL19 before adoptive transfer. The desensitized cells homed to BALB/c-plt/plt PLNs as efficiently as control cells (unpublished data), indicating that these chemokines do not play a role here. Other possible candidates include CXCR3 and its ligands as well as lipid chemoattractants, such as LTB4, which contribute to effector T cell homing to sites of inflammation (28). However, homing of CXCR3−/− TCM was identical to that of WT TCM (unpublished data), and TCM do not chemotax toward LTB4 gradients (28). Thus, the nature of other chemoattractants involved in CCR7-independent TCM homing to PLNs remains to be identified.

Together, these results strongly suggest that CXCL12–CXCR4 triggers integrin activation on rolling TCM, but not on naive T cells, in PLN HEVs of DDD/1-plt/plt mice. This pathway accounts for a significant fraction of TCM that home to PLNs. In addition, there is probably at least one other PTX-sensitive recruitment signal whose identity remains to be uncovered.

A Physiological Role for CXCL12 in TCM Homing to PLNs.

Given the severely disturbed architecture of secondary lymphoid organs in plt/plt mice (9, 10), we asked whether the observed contribution by CXCL12 to TCM migration reflects a truly physiological event. Therefore, we examined the effect of CXCL12 inhibition on TCM homing to WT PLNs. Indeed, homing was moderately by ∼20%, but significantly, reduced (Fig. 4 E, P < 0.05). This reduction in homing occurred in both C57BL/6 and BALB/c mice, indicating that the role of CXCL12 in WT mice may not depend on genetic modifiers.

We conclude that the CCR7 pathway plays a dominant role in TCM homing to PLNs, but CXCL12 also contributes to this process. Our finding that this nonredundant effect of CXCL12 is specific for TCM is also supported by earlier conclusions that CXCR4 deficiency does not reduce the ability of naive T cells to home to PLNs that express normal CCR7 ligands (20).

CXCL12 Induces Firm Adherence of TCM, But Not Naive T Cells, in Cremaster Muscle Venules.

Why are naive T cells apparently incapable of using CXCL12 for homing to PLNs? Naive T cells respond to CXCL12 gradients in chemotaxis assays at least as efficiently as TCM (unpublished data). Furthermore, CXCL12 induces LFA-1–mediated adhesion of human naive T cells to ICAM-1 in flow chamber assays (29). In contrast, murine naive T cells fail to arrest in cremaster muscle venules upon CXCL12 superfusion, but undergo immediate arrest in muscles superfused with CCL21 (16).

Because our homing data also indicate that CXCL12 fails to attract naive T cells to PLNs, but can recruit TCM, we performed IVM to determine whether circulating TCM can respond to CXCL12 in situ. To this end, we asked whether CXCL12 superfusion induces TCM sticking in cremaster muscle venules (16). Fluorescently labeled TCM were injected intraarterially and their interactions with cremaster muscle venules were recorded. Without CXCL12 superfusion, the mean rolling and sticking fractions were 18.3 ± 5.0% and 1.3 ± 0.9%, respectively (n = 3 mice/30 vessels). Upon subsequent superfusion with CXCL12, rolling fractions remained unchanged (Fig. 5 

A, 23.9 ± 3.0%, P > 0.05). In contrast, the TCM sticking fraction was significantly increased (Fig. 5 B, 6.9 ± 1.8%, P < 0.05 vs. control). To determine whether this was an effect of CXCL12 on the venular endothelium or on circulating TCM, we desensitized TCM to CXCL12 before injection. Desensitized TCM rolled similarly to untreated cells, but were incapable of sticking (Fig. 5, A and B). Thus, TCM, unlike naive T cells, are responsive to CXCL12 displayed on venular endothelium.

What could account for the distinct intravascular responsiveness of TCM versus naive T cells to CXCL12? One possibility is that these two subsets express distinct densities of CXCR4. Indeed, flow cytometry revealed that TCM expressed markedly higher levels of CXCR4 compared with naive CD8+ T cells (Fig. 5 C; mean fluorescence intensity: TCM, 278 and naive T cells, 126). This finding is also in accordance with a recent microarray analysis of TCM and naive T cells, which showed higher mRNA expression of CXCR4 in the former subset (30).

What might be the functional relevance of CCR7-independent homing of TCM to PLNs? Wherry et al. have recently shown that CD8+ effector CTL can give rise to TEM, which differentiate into L-selectin+ TCM over the course of several weeks (5). Effector CTL and TEM do not express L-selectin and CCR7 (4, 6, 8). Although the kinetics of L-selectin and CCR7 induction on TEM in the course of their transition into TCM is unknown, it has been shown that some Ag-experienced T cells express L-selectin but not CCR7 (4). For this subset, expression of CXCR4 could provide an alternative pathway to gain CCR7-independent access to PLNs. Moreover, CXCL12 is constitutively expressed in many tissues, especially in the BM, and is up-regulated during inflammation. In addition, human TCM isolated ex vivo, and murine TCM generated in vitro express several inflammatory chemokine receptors (4, 8). This broad expression pattern of chemokine receptors provides TCM with maximum flexibility to survey not only secondary lymphoid organs but also multiple other sites in the body.


The authors thank L. Cavanagh and R. Bonasio for helpful discussions, A. Matsuzawa for plt/plt mice, C. Gerard for CXCR3−/− mice, B. Reinhardt and G. Cheng for technical support, and J. Moore for editorial assistance.

T.W. Felbinger was supported by a research stipend from the Deutsche Forshungsgemeinschaft. W. Weninger was supported by an Erwin-Schrödinger Auslandstipendium from the Austrian Science Foundation and by a Pilot and Feasibility grant from the Harvard Skin Disease Research Center. U.H. von Andrian was supported by National Institutes of Health grants HL48675, HL54936, and HL56949.


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M.L. Scimone and T.W. Felbinger contributed equally to this work.

The present address of J.V. Stein is National Center for Biotechnology, CNB/CSIC, 28049 Madrid, Spain.

The present address of W. Weninger is The Wistar Institute, Philadelphia, PA 19104.

Abbreviations used in this paper: GFP, green fluorescent protein; GPCR, Gαi-protein coupled receptor; HEV, high endothelial venule; IVM, intravital microscopy; MLN, mesenteric LN; PLN, peripheral LN; plt, paucity of lymph node T cells; PTX, pertussis toxin; TCM, central memory T cells; TEM, effector memory T cells; TRITC, tetramethylrhodamine-5-isothiocyanate.