Secondary lymphoid tissue organogenesis requires tumor necrosis factor (TNF) and lymphotoxin α (LTα). The role of TNF in B cell positioning and formation of follicular structure was studied by comparing the location of newly produced naive recirculating and antigen-stimulated B cells in TNF−/− and TNF/LTα−/− mice. By creating radiation bone marrow chimeras from wild-type and TNF−/− mice, formation of normal splenic B cell follicles was shown to depend on TNF production by radiation-sensitive cells of hemopoietic origin. Reciprocal adoptive transfers of mature B cells between wild-type and knockout mice indicated that normal follicular tropism of recirculating naive B cells occurs independently of TNF derived from the recipient spleen. Moreover, soluble TNF receptor–IgG fusion protein administered in vivo failed to prevent B cell localization to the follicle or the germinal center reaction. Normal T zone tropism was observed when antigen-stimulated B cells were transferred into TNF−/− recipients, but not into TNF/LTα−/− recipients. This result appeared to account for the defect in isotype switching observed in intact TNF/LTα−/− mice because TNF/LTα−/− B cells, when stimulated in vitro, switched isotypes normally. Thus, TNF is necessary for creating the permissive environment for B cell movement and function, but is not itself responsible for these processes.
Skip Nav Destination
Article navigation
19 October 1998
Article|
October 19 1998
Generation of Splenic Follicular Structure and B Cell Movement in Tumor Necrosis Factor–deficient Mice
Matthew C. Cook,
Matthew C. Cook
From the *Centenary Institute of Cancer Medicine and Cell Biology, Sydney, NSW, Australia 2050; the ‡Medical Foundation of the University of Sydney, Sydney, NSW, Australia 2050; and the §Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, California 94143
Search for other works by this author on:
Heinrich Körner,
Heinrich Körner
From the *Centenary Institute of Cancer Medicine and Cell Biology, Sydney, NSW, Australia 2050; the ‡Medical Foundation of the University of Sydney, Sydney, NSW, Australia 2050; and the §Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, California 94143
Search for other works by this author on:
D. Sean Riminton,
D. Sean Riminton
From the *Centenary Institute of Cancer Medicine and Cell Biology, Sydney, NSW, Australia 2050; the ‡Medical Foundation of the University of Sydney, Sydney, NSW, Australia 2050; and the §Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, California 94143
Search for other works by this author on:
Frances A. Lemckert,
Frances A. Lemckert
From the *Centenary Institute of Cancer Medicine and Cell Biology, Sydney, NSW, Australia 2050; the ‡Medical Foundation of the University of Sydney, Sydney, NSW, Australia 2050; and the §Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, California 94143
Search for other works by this author on:
Jhagvaral Hasbold,
Jhagvaral Hasbold
From the *Centenary Institute of Cancer Medicine and Cell Biology, Sydney, NSW, Australia 2050; the ‡Medical Foundation of the University of Sydney, Sydney, NSW, Australia 2050; and the §Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, California 94143
Search for other works by this author on:
Michelle Amesbury,
Michelle Amesbury
From the *Centenary Institute of Cancer Medicine and Cell Biology, Sydney, NSW, Australia 2050; the ‡Medical Foundation of the University of Sydney, Sydney, NSW, Australia 2050; and the §Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, California 94143
Search for other works by this author on:
Philip D. Hodgkin,
Philip D. Hodgkin
From the *Centenary Institute of Cancer Medicine and Cell Biology, Sydney, NSW, Australia 2050; the ‡Medical Foundation of the University of Sydney, Sydney, NSW, Australia 2050; and the §Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, California 94143
Search for other works by this author on:
Jason G. Cyster,
Jason G. Cyster
From the *Centenary Institute of Cancer Medicine and Cell Biology, Sydney, NSW, Australia 2050; the ‡Medical Foundation of the University of Sydney, Sydney, NSW, Australia 2050; and the §Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, California 94143
Search for other works by this author on:
Jonathon D. Sedgwick,
Jonathon D. Sedgwick
From the *Centenary Institute of Cancer Medicine and Cell Biology, Sydney, NSW, Australia 2050; the ‡Medical Foundation of the University of Sydney, Sydney, NSW, Australia 2050; and the §Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, California 94143
Search for other works by this author on:
Antony Basten
Antony Basten
From the *Centenary Institute of Cancer Medicine and Cell Biology, Sydney, NSW, Australia 2050; the ‡Medical Foundation of the University of Sydney, Sydney, NSW, Australia 2050; and the §Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, California 94143
Search for other works by this author on:
Matthew C. Cook
From the *Centenary Institute of Cancer Medicine and Cell Biology, Sydney, NSW, Australia 2050; the ‡Medical Foundation of the University of Sydney, Sydney, NSW, Australia 2050; and the §Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, California 94143
Heinrich Körner
From the *Centenary Institute of Cancer Medicine and Cell Biology, Sydney, NSW, Australia 2050; the ‡Medical Foundation of the University of Sydney, Sydney, NSW, Australia 2050; and the §Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, California 94143
D. Sean Riminton
From the *Centenary Institute of Cancer Medicine and Cell Biology, Sydney, NSW, Australia 2050; the ‡Medical Foundation of the University of Sydney, Sydney, NSW, Australia 2050; and the §Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, California 94143
Frances A. Lemckert
From the *Centenary Institute of Cancer Medicine and Cell Biology, Sydney, NSW, Australia 2050; the ‡Medical Foundation of the University of Sydney, Sydney, NSW, Australia 2050; and the §Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, California 94143
Jhagvaral Hasbold
From the *Centenary Institute of Cancer Medicine and Cell Biology, Sydney, NSW, Australia 2050; the ‡Medical Foundation of the University of Sydney, Sydney, NSW, Australia 2050; and the §Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, California 94143
Michelle Amesbury
From the *Centenary Institute of Cancer Medicine and Cell Biology, Sydney, NSW, Australia 2050; the ‡Medical Foundation of the University of Sydney, Sydney, NSW, Australia 2050; and the §Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, California 94143
Philip D. Hodgkin
From the *Centenary Institute of Cancer Medicine and Cell Biology, Sydney, NSW, Australia 2050; the ‡Medical Foundation of the University of Sydney, Sydney, NSW, Australia 2050; and the §Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, California 94143
Jason G. Cyster
From the *Centenary Institute of Cancer Medicine and Cell Biology, Sydney, NSW, Australia 2050; the ‡Medical Foundation of the University of Sydney, Sydney, NSW, Australia 2050; and the §Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, California 94143
Jonathon D. Sedgwick
From the *Centenary Institute of Cancer Medicine and Cell Biology, Sydney, NSW, Australia 2050; the ‡Medical Foundation of the University of Sydney, Sydney, NSW, Australia 2050; and the §Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, California 94143
Antony Basten
From the *Centenary Institute of Cancer Medicine and Cell Biology, Sydney, NSW, Australia 2050; the ‡Medical Foundation of the University of Sydney, Sydney, NSW, Australia 2050; and the §Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, California 94143
Address correspondence to Antony Basten or Jonathon D. Sedgwick, Centenary Institute of Cancer Medicine and Cell Biology, Bldg. 93, Royal Prince Alfred Hospital, Missenden Rd., Camperdown, Sydney, NSW 2050 Australia. Phone: 61-2-9565-6155; Fax: 61-2-9565-6105; E-mail: [email protected] or [email protected]
M.C. Cook's present address is Department of Immunology, University of Birmingham, Medical School, Edgbaston, Birmingham, B15 2TT, UK. H. Körner's present address is Institute for Microbiology, Hygiene and Immunology, Wasserturmstrasse 3, D-91054 Erlangen, Germany.
M.C. Cook and H. Körner contributed equally to this study.
Received:
May 13 1998
Revision Received:
August 14 1998
Online ISSN: 1540-9538
Print ISSN: 0022-1007
1998
J Exp Med (1998) 188 (8): 1503–1510.
Article history
Received:
May 13 1998
Revision Received:
August 14 1998
Citation
Matthew C. Cook, Heinrich Körner, D. Sean Riminton, Frances A. Lemckert, Jhagvaral Hasbold, Michelle Amesbury, Philip D. Hodgkin, Jason G. Cyster, Jonathon D. Sedgwick, Antony Basten; Generation of Splenic Follicular Structure and B Cell Movement in Tumor Necrosis Factor–deficient Mice . J Exp Med 19 October 1998; 188 (8): 1503–1510. doi: https://doi.org/10.1084/jem.188.8.1503
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionSuggested Content
Email alerts
Advertisement
