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1-4 of 4
Makoto M. Taketo
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Journal Articles
Pallavi Bhattaram, Alfredo Penzo-Méndez, Kenji Kato, Kaustav Bandyopadhyay, Abhilash Gadi, Makoto M. Taketo, Véronique Lefebvre
Journal:
Journal of Cell Biology
Journal of Cell Biology (2014) 207 (5): 657–671.
Published: 01 December 2014
Abstract
Canonical WNT signaling stabilizes β-catenin to determine cell fate in many processes from development onwards. One of its main roles in skeletogenesis is to antagonize the chondrogenic transcription factor SOX9. We here identify the SOXC proteins as potent amplifiers of this pathway. The SOXC genes, i.e., Sox4 , Sox11 , and Sox12 , are coexpressed in skeletogenic mesenchyme, including presumptive joints and perichondrium, but not in cartilage. Their inactivation in mouse embryo limb bud caused massive cartilage fusions, as joint and perichondrium cells underwent chondrogenesis. SOXC proteins govern these cells cell autonomously. They replace SOX9 in the adenomatous polyposis coli–Axin destruction complex and therein inhibit phosphorylation of β-catenin by GSK3. This inhibition, a crucial, limiting step in canonical WNT signaling, thus becomes a constitutive event. The resulting SOXC/canonical WNT-mediated synergistic stabilization of β-catenin contributes to efficient repression of Sox9 in presumptive joint and perichondrium cells and thereby ensures proper delineation and articulation of skeletal primordia. This synergy may determine cell fate in many processes besides skeletogenesis.
Includes: Supplementary data
Journal Articles
Stefan Liebner, Monica Corada, Thorsten Bangsow, Jane Babbage, Andrea Taddei, Cathrin J. Czupalla, Marco Reis, Angelina Felici, Hartwig Wolburg, Marcus Fruttiger, Makoto M. Taketo, Harald von Melchner, Karl Heinz Plate, Holger Gerhardt, Elisabetta Dejana
Journal:
Journal of Cell Biology
Journal of Cell Biology (2008) 183 (3): 409–417.
Published: 27 October 2008
Abstract
The blood–brain barrier (BBB) is confined to the endothelium of brain capillaries and is indispensable for fluid homeostasis and neuronal function. In this study, we show that endothelial Wnt/β-catenin (β-cat) signaling regulates induction and maintenance of BBB characteristics during embryonic and postnatal development. Endothelial specific stabilization of β-cat in vivo enhances barrier maturation, whereas inactivation of β-cat causes significant down-regulation of claudin3 (Cldn3), up-regulation of plamalemma vesicle-associated protein, and BBB breakdown. Stabilization of β-cat in primary brain endothelial cells (ECs) in vitro by N-terminal truncation or Wnt3a treatment increases Cldn3 expression, BBB-type tight junction formation, and a BBB characteristic gene signature. Loss of β-cat or inhibition of its signaling abrogates this effect. Furthermore, stabilization of β-cat also increased Cldn3 and barrier properties in nonbrain-derived ECs. These findings may open new therapeutic avenues to modulate endothelial barrier function and to limit the devastating effects of BBB breakdown.
Includes: Supplementary data
Journal Articles
Yoshihiko Shimizu, Dean Thumkeo, Jeongsin Keel, Toshimasa Ishizaki, Hiroko Oshima, Masanobu Oshima, Yoichi Noda, Fumio Matsumura, Makoto M. Taketo, Shuh Narumiya
Journal:
Journal of Cell Biology
Journal of Cell Biology (2005) 168 (6): 941–953.
Published: 07 March 2005
Abstract
Rho-associated kinase (ROCK) I mediates signaling from Rho to the actin cytoskeleton. To investigate the in vivo functions of ROCK-I, we generated ROCK-I–deficient mice. Loss of ROCK-I resulted in failure of eyelid closure and closure of the ventral body wall, which gave rise to the eyes open at birth and omphalocele phenotypes in neonates. Most ROCK-I −/− mice died soon after birth as a result of cannibalization of the omphalocele by the mother. Actin cables that encircle the eye in the epithelial cells of the eyelid were disorganized and accumulation of filamentous actin at the umbilical ring was impaired, with loss of phosphorylation of the myosin regulatory light chain (MLC) at both sites, in ROCK-I −/− embryos. Stress fiber formation and MLC phosphorylation induced by EGF were also attenuated in primary keratinocytes from ROCK-I −/− mice. These results suggest that ROCK-I regulates closure of the eyelids and ventral body wall through organization of actomyosin bundles.
Journal Articles
Yoshitaka Tamai, Tomo-o Ishikawa, Michael R. Bösl, Masahiko Mori, Masami Nozaki, Heléne Baribault, Robert G. Oshima, Makoto M. Taketo
Journal:
Journal of Cell Biology
Journal of Cell Biology (2000) 151 (3): 563–572.
Published: 30 October 2000
Abstract
To investigate the expression and biological roles of cytokeratin 19 (K19) in development and in adult tissues, we inactivated the mouse K19 gene ( Krt1-19 ) by inserting a bacterial β-galactosidase gene ( lacZ ) by homologous recombination in embryonic stem cells, and established germ line mutant mice. Both heterozygous and homozygous mutant mice were viable, fertile, and appeared normal. By 7.5–8.0 days post coitum (dpc), heterozygous mutant embryos expressed lacZ in the notochordal plate and hindgut diverticulum, reflecting the fact that the notochord and the gut endoderm are derived from the axial mesoderm-originated cells. In the adult mutant, lacZ was expressed mainly in epithelial tissues. To investigate the possible functional cooperation and synergy between K19 and K8, we then constructed compound homozygous mutants, whose embryos died ∼10 dpc. The lethality resulted from defects in the placenta where both K19 and K8 are normally expressed. As early as 9.5 dpc, the compound mutant placenta had an excessive number of giant trophoblasts, but lacked proper labyrinthine trophoblast or spongiotrophoblast development, which apparently caused flooding of the maternal blood into the embryonic placenta. These results indicate that K19 and K8 cooperate in ensuring the normal development of placental tissues.