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1-7 of 7
Denis Wirtz
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Journal Articles
Nicolas A. Perez-Gonzalez, Nash D. Rochman, Kai Yao, Jiaxiang Tao, Minh-Tam Tran Le, Shannon Flanary, Lucia Sablich, Ben Toler, Eliana Crentsil, Felipe Takaesu, Bram Lambrus, Jessie Huang, Vivian Fu, Pragati Chengappa, Tia M. Jones, Andrew J. Holland, Steven An, Denis Wirtz, Ryan J. Petrie, Kun-Liang Guan, Sean X. Sun
Journal:
Journal of Cell Biology
Journal of Cell Biology (2019) 218 (10): 3472–3488.
Published: 03 September 2019
Abstract
How mammalian cells regulate their physical size is currently poorly understood, in part due to the difficulty in accurately quantifying cell volume in a high-throughput manner. Here, using the fluorescence exclusion method, we demonstrate that the mechanosensitive transcriptional regulators YAP (Yes-associated protein) and TAZ (transcriptional coactivator with PDZ-binding motif) are regulators of single-cell volume. The role of YAP/TAZ in volume regulation must go beyond its influence on total cell cycle duration or cell shape to explain the observed changes in volume. Moreover, for our experimental conditions, volume regulation by YAP/TAZ is independent of mTOR. Instead, we find that YAP/TAZ directly impacts the cell division volume, and YAP is involved in regulating intracellular cytoplasmic pressure. Based on the idea that YAP/TAZ is a mechanosensor, we find that inhibiting myosin assembly and cell tension slows cell cycle progression from G1 to S. These results suggest that YAP/TAZ may be modulating cell volume in combination with cytoskeletal tension during cell cycle progression.
Includes: Supplementary data
Journal Articles
Journal:
Journal of Cell Biology
Journal of Cell Biology (2019) 218 (4): 1092–1093.
Published: 14 March 2019
Abstract
Persistent cell migration plays a crucial role in physiological processes, but its underlying mechanisms of regulation remain unclear. Mason et al. (2019. J. Cell Biol. https://doi.org/10.1083/jcb.201806065 ) show that YAP/TAZ limit cytoskeletal tension, which is essential for persistent (but not initiation of) cell migration. Potential implications of this study range from embryonic development to metastasis.
Journal Articles
Vivian C. Hecht, Lucas B. Sullivan, Robert J. Kimmerling, Dong-Hwee Kim, Aaron M. Hosios, Max A. Stockslager, Mark M. Stevens, Joon Ho Kang, Denis Wirtz, Matthew G. Vander Heiden, Scott R. Manalis
Journal:
Journal of Cell Biology
Journal of Cell Biology (2016) 212 (4): 439–447.
Published: 15 February 2016
Abstract
Cytokine regulation of lymphocyte growth and proliferation is essential for matching nutrient consumption with cell state. Here, we examine how cellular biophysical changes that occur immediately after growth factor depletion promote adaptation to reduced nutrient uptake. After growth factor withdrawal, nutrient uptake decreases, leading to apoptosis. Bcl-x L expression prevents cell death, with autophagy facilitating long-term cell survival. However, autophagy induction is slow relative to the reduction of nutrient uptake, suggesting that cells must engage additional adaptive mechanisms to respond initially to growth factor depletion. We describe an acute biophysical response to growth factor withdrawal, characterized by a simultaneous decrease in cell volume and increase in cell density, which occurs before autophagy initiation and is observed in both FL5.12 Bcl-x L cells depleted of IL-3 and primary CD8 + T cells depleted of IL-2 that are differentiating toward memory cells. The response reduces cell surface area to minimize energy expenditure while conserving biomass, suggesting that the biophysical properties of cells can be regulated to promote survival under conditions of nutrient stress.
Includes: Supplementary data
Journal Articles
Journal:
Journal of Cell Biology
Journal of Cell Biology (2009) 184 (4): 473–479.
Published: 16 February 2009
Abstract
To generate cellular diversity in developing organisms while simultaneously maintaining the developmental potential of the germline, germ cells must be able to preferentially endow germline daughter cells with a cytoplasmic portion containing specialized cell fate determinants not inherited by somatic cells. In Caenorhabditis elegans , germline inheritance of the protein PIE-1 is accomplished by first asymmetrically localizing the protein to the germplasm before cleavage and subsequently degrading residual levels of the protein in the somatic cytoplasm after cleavage. Despite its critical involvement in cell fate determination, the enrichment of germline determinants remains poorly understood. Here, combining live-cell fluorescence methods and kinetic modeling, we demonstrate that the enrichment process does not involve protein immobilization, intracellular compartmentalization, or localized protein degradation. Instead, our results support a heterogeneous reaction/diffusion model for PIE-1 enrichment in which the diffusion coefficient of PIE-1 is reversibly reduced in the posterior, resulting in a stable protein gradient across the zygote at steady state.
Includes: Supplementary data
Journal Articles
Stephanie L. Gupton, Karen L. Anderson, Thomas P. Kole, Robert S. Fischer, Aaron Ponti, Sarah E. Hitchcock-DeGregori, Gaudenz Danuser, Velia M. Fowler, Denis Wirtz, Dorit Hanein, Clare M. Waterman-Storer
Journal:
Journal of Cell Biology
Journal of Cell Biology (2005) 168 (4): 619–631.
Published: 14 February 2005
Abstract
The actin cytoskeleton is locally regulated for functional specializations for cell motility. Using quantitative fluorescent speckle microscopy (qFSM) of migrating epithelial cells, we previously defined two distinct F-actin networks based on their F-actin–binding proteins and distinct patterns of F-actin turnover and movement. The lamellipodium consists of a treadmilling F-actin array with rapid polymerization-dependent retrograde flow and contains high concentrations of Arp2/3 and ADF/cofilin, whereas the lamella exhibits spatially random punctae of F-actin assembly and disassembly with slow myosin-mediated retrograde flow and contains myosin II and tropomyosin (TM). In this paper, we microinjected skeletal muscle αTM into epithelial cells, and using qFSM, electron microscopy, and immunolocalization show that this inhibits functional lamellipodium formation. Cells with inhibited lamellipodia exhibit persistent leading edge protrusion and rapid cell migration. Inhibition of endogenous long TM isoforms alters protrusion persistence. Thus, cells can migrate with inhibited lamellipodia, and we suggest that TM is a major regulator of F-actin functional specialization in migrating cells.
Includes: Supplementary data
Journal Articles
Journal:
Journal of Cell Biology
Journal of Cell Biology (2003) 160 (5): 635–644.
Published: 25 February 2003
Abstract
The RanGTP gradient across the interphase nuclear envelope and on the condensed mitotic chromosomes is essential for many cellular processes, including nucleocytoplasmic transport and spindle assembly. Although the chromosome-associated enzyme RCC1 is responsible for RanGTP production, the mechanism of generating and maintaining the RanGTP gradient in vivo remains unknown. Here, we report that regulator of chromosome condensation (RCC1) rapidly associates and dissociates with both interphase and mitotic chromosomes in living cells, and that this mobility is regulated during the cell cycle. Our kinetic modeling suggests that RCC1 couples its catalytic activity to chromosome binding to generate a RanGTP gradient. Indeed, we have demonstrated experimentally that the interaction of RCC1 with the chromatin is coupled to the nucleotide exchange on Ran in vivo. The coupling is due to the stable binding of the binary complex of RCC1–Ran to chromatin. Successful nucleotide exchange dissociates the binary complex, permitting the release of RCC1 and RanGTP from the chromatin and the production of RanGTP on the chromatin surface.
Includes: Supplementary data
Journal Articles
Olivier Bousquet, Linglei Ma, Soichiro Yamada, Changhong Gu, Toshihiro Idei, Kenzo Takahashi, Denis Wirtz, Pierre A. Coulombe
Journal:
Journal of Cell Biology
Journal of Cell Biology (2001) 155 (5): 747–754.
Published: 26 November 2001
Abstract
Keratin filaments arise from the copolymerization of type I and II sequences, and form a pancytoplasmic network that provides vital mechanical support to epithelial cells. Keratins 5 and 14 are expressed as a pair in basal cells of stratified epithelia, where they occur as bundled arrays of filaments. In vitro, bundles of K5–K14 filaments can be induced in the absence of cross-linkers, and exhibit enhanced resistance to mechanical strain. This property is not exhibited by copolymers of K5 and tailless K14, in which the nonhelical tail domain has been removed, or copolymers of K5 and K19, a type I keratin featuring a short tail domain. The purified K14 tail domain binds keratin filaments in vitro with specificity (kD ∼2 μM). When transiently expressed in cultured cells, the K14 tail domain associates with endogenous keratin filaments. Utilization of the K14 tail domain as a bait in a yeast two-hybrid screen pulls out type I keratin sequences from a skin cDNA library. These data suggest that the tail domain of K14 contributes to the ability of K5–K14 filaments to self-organize into large bundles showing enhanced mechanical resilience in vitro.