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Cover Image
Cover Image
On the cover
Fernández-Busnadiego et al. present a three-dimensional view of the presynaptic cytomatrix using cryo-electron tomography. Synaptic vesicles (yellow) are linked to each other by short connector filaments (red) and docked at the plasma membrane (purple) by protein tethers (dark blue).
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In This Issue
In Focus
The integrin reconstruction act
Recreating integrin activation in vitro resolves several long-running controversies.
People & Ideas
Pier Paolo Di Fiore: Plumbing the depths of endocytosis
Di Fiore specializes in fishing out connections between the endocytic machinery and cancer.
Review
Report
The cytoplasmic tail of fibrocystin contains a ciliary targeting sequence
An 18-residue motif in the cytoplasmic tail of polycystic kidney disease gene product, fibrocystin, targets it to ciliary membranes through interactions with Rab8.
Drosophila Kelch functions with Cullin-3 to organize the ring canal actin cytoskeleton
In addition to cross-linking F-actin, Drosophila Kelch is a component of a cullin-RING ubiquitin ligase complex required for morphogenesis of ring canals during oogenesis.
Article
Activation of Cdc6 by MyoD is associated with the expansion of quiescent myogenic satellite cells
Cdc6, which alters chromatin ultrastructure to allow DNA replication in muscle stem cells transitioning out of quiescence, is identified as a target of the MyoD transcription factor.
Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope
The lamin-B nuclear envelope stabilizes spindle microtubules by keeping the competitive motility of opposing-force kinesins in check.
Integrating high-throughput genetic interaction mapping and high-content screening to explore yeast spindle morphogenesis
A combination of yeast genetics, synthetic genetic array analysis, and high-throughput screening reveals that sumoylation of Mcm21p promotes disassembly of the mitotic spindle.
Overexpression of the E2 ubiquitin–conjugating enzyme UbcH10 causes chromosome missegregation and tumor formation
An overabundance of UbcH10 disrupts mitotic checkpoint signaling as a result of a degradation of cyclin B, increasing spontaneous and carcinogen-induced tumor formation in transgenic mice.
The conserved oligomeric Golgi complex is involved in double-membrane vesicle formation during autophagy
COG subunits localize to the phagophore assembly site where they interact with autophagy proteins and are required for double-membrane Cvt vesicle and autophagosome formation.
Neuropilin-2 mediates VEGF-C–induced lymphatic sprouting together with VEGFR3
If neuropilin-2 and the growth factor VEGF-C don’t come together, lymphatic vessels don’t branch apart.
The clathrin heavy chain isoform CHC22 functions in a novel endosomal sorting step
CHC22 is needed for retrograde trafficking from endosomes to the trans-Golgi, unlike its clathrin sibling CHC17, and in a manner distinct from retromer.
Quantitative analysis of the native presynaptic cytomatrix by cryoelectron tomography
The filamentous structures that tether exocytic vesicles to the plasma membrane in the active zone are rearranged in response to synaptic stimulation.
Recreation of the terminal events in physiological integrin activation
In vitro analysis confirms talin binding is sufficient for activation and extension of membrane-embedded integrin.
