In the first collection celebrating our 65th anniversary, we revisit classic methods that shaped cell biological research, including a fundamentally important technique for electron microscopy that has proved to be critical for all areas of cell biology, the development of laser scanning confocal imaging, the description of the TUNEL assay and early uses of the Lac O/Lac I system to visualize chromatin structure and dynamics. The collection highlights recent techniques refining the toolboxes of today's cell biologists, such as the now-common BioID method probing protein–protein interactions and the introduction of nanobodies as an alternative to anti–immunoglobulin G secondary antibodies. If we missed your favorite JCB study, tweet to @JCellBiol using the hashtag #JCB65, or e-mail us at email@example.com. Find more 65th anniversary collections or browse our From the Archive series.
Image from Reynolds, 1963.