Infection of baby hamster kidney cells with vesicular stomatitis virus (VSV) caused a reduced rate of pinocytosis (as judged by the uptake of horseradish peroxidase) after 1 h, and maximum inhibition (60-80%) was observed at 4-6 h. This inhibition occurred 2-3 h before release of virus or changes in cell morphology. Analytical cell fractionation of homogenates of VSV-infected cells indicated that the horseradish peroxidase taken up by pinocytosis was transferred to lysosomes. The inhibition of pinocytosis required viral gene expression: little or no inhibition was detected in cells infected with UV-irradiated virus, wild-type virus in the presence of cycloheximide, or a temperature-sensitive mutant which failed to synthesize viral proteins. When cells were infected with temperature-sensitive viruses with mutations in the five VSV genes, an inhibition of pinocytosis was observed only when the viral transmembrane glycoprotein was present on the surface of the cells.
Rapid inhibition of pinocytosis in baby hamster kidney (BHK-21) cells following infection with vesicular stomatitis virus.
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D K Wilcox, P A Whitaker-Dowling, J S Youngner, C C Widnell; Rapid inhibition of pinocytosis in baby hamster kidney (BHK-21) cells following infection with vesicular stomatitis virus.. J Cell Biol 1 November 1983; 97 (5): 1444–1451. doi: https://doi.org/10.1083/jcb.97.5.1444
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