We show that freeze-dried actin filaments which have been rotary shadowed with a light coat of platinum appear very similar in morphology and width to negatively-stained filaments. The addition of a thicker coat of platinum to such preparations gives the actin filaments a different morphology and width, which are similar to those of the rotary-shadowed, quick-frozen filaments described by Heuser and Kirschner (J. Cell Biol. 1980, 86:212-234). The consistent view of the actin filament presented here, particularly its 7-8-nm width, can be interpreted in terms of the overall orientation of the actin subunit in the actin filament.
Article| July 01 1983
A consistent picture of the actin filament related to the orientation of the actin molecule.
W E Fowler
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1983) 97 (1): 264–269.
W E Fowler, U Aebi; A consistent picture of the actin filament related to the orientation of the actin molecule.. J Cell Biol 1 July 1983; 97 (1): 264–269. doi: https://doi.org/10.1083/jcb.97.1.264
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