Golgi-associated processing of complex-type oligosaccharides linked to asparagine involves the sequential action of at least six enzymes. By equilibrium sucrose density gradient centrifugation of membranes from Chinese hamster ovary cells, we have partially resolved the set of four initial enzymes in the pathway (Mannosidase I, N-acetylglucosamine (GlcNAc) Transferase I, Mannosidase II, and GlcNAc Transferase II) from two later-acting activities (galactosyltransferase and sialyltransferase). In view of the recent demonstration that galactosyltransferase is restricted to the trans face of the Golgi complex in HeLa cells (Roth, J., and E.G. Berger, 1982, J. Cell Biol., 93:223-229), our results suggest that removal of mannose and attachment of peripheral N-acetylglucosamine may occur in some or all of the remaining cisternae on the cis side of the Golgi stack.
Article| July 01 1983
Compartmentation of asparagine-linked oligosaccharide processing in the Golgi apparatus.
W G Dunphy
J E Rothman
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1983) 97 (1): 270–275.
W G Dunphy, J E Rothman; Compartmentation of asparagine-linked oligosaccharide processing in the Golgi apparatus.. J Cell Biol 1 July 1983; 97 (1): 270–275. doi: https://doi.org/10.1083/jcb.97.1.270
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