Rotary replication has been adapted to freeze-etching and evaluated using T4 polyheads, erythrocyte ghosts, and chloroplast membranes. Conventional electron microscopy, electron diffraction, and optical diffraction and filtering indicate that platinum-carbon rotary replication renders radially symmetrical contrast and 25 A resolution to freeze-etched specimens so as to clarify subunit structure not normally evident in unidirectional shadow replicas.
Article| January 01 1977
Rotary replication for freeze-etching.
L H Margaritis
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1977) 72 (1): 47–56.
L H Margaritis, A Elgsaeter, D Branton; Rotary replication for freeze-etching.. J Cell Biol 1 January 1977; 72 (1): 47–56. doi: https://doi.org/10.1083/jcb.72.1.47
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