We have developed a method for permeabilizing CHO cells to nucleotides under conditions which allow most cells to remain viable. Permeabilized cells can carry out ATP-dependent, semiconservative synthesis of DNA. The data are consistent with the continuation of DNA synthesis in those cells in S phase at the time of treatment, possibly limited to completion of replicon synthesis without new initiations.
Article| June 01 1976
DNA replication in Chinese hamster ovary cells made permeable to nucleotides by tween-80 treatment.
A C Olson
Online Issn: 1540-8140
Print Issn: 0021-9525
J Cell Biol (1976) 69 (3): 732–736.
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D Billen, A C Olson; DNA replication in Chinese hamster ovary cells made permeable to nucleotides by tween-80 treatment.. J Cell Biol 1 June 1976; 69 (3): 732–736. doi: https://doi.org/10.1083/jcb.69.3.732
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