Membrane vesicles with a high mating reactivity were obtained from cilia of Paramecium caudatum by treatment with a solution containing 2 M urea and 0.1 mM Na2-EDTA. All processes of conjugation were induced in cells of the complementary mating type by approximately 10 mug/ml proteins of the vesicles. Electron microscope observation showed that the membrane vesicles have a diameter of 100-150 nm. Electrophoretic analysis on SDS polyacrylamide gel revealed no significant difference in polypeptide patterns of the particles from the two complementary mating types.
Article| June 01 1976
Mating-reactive membrane vesicles from cilia of Paramecium caudatum.
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1976) 69 (3): 736–740.
A Kitamura, K Hiwatashi; Mating-reactive membrane vesicles from cilia of Paramecium caudatum.. J Cell Biol 1 June 1976; 69 (3): 736–740. doi: https://doi.org/10.1083/jcb.69.3.736
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