The series introduced by this paper reports the results of a detailed analysis of the microsomal fraction from rat liver by density gradient centrifugation. The biochemical methods used throughout this work for the determination of monoamine oxidase, NADH cytochrome c reductase, NADPH cytochrome c reductase, cytochrome oxidase, catalase, aminopyrine demethylase, cytochromes b5 and P 450, glucuronyltransferase, galactosyltransferase, esterase, alkaline and acid phosphatases, 5'-nucleotidase, glucose 6-phosphatase, alkaline phosphodiesterase I, N-acetyl-ß-glucosaminidase, ß-glucuronidase, nucleoside diphosphatase, aldolase, fumarase, glutamine synthetase, protein, phospholipid, cholesterol, and RNA are described and justified when necessary.
ANALYTICAL STUDY OF MICROSOMES AND ISOLATED SUBCELLULAR MEMBRANES FROM RAT LIVER : I. Biochemical Methods
- Views Icon Views
- PDF LinkPDF
- Share Icon Share
- Search Site
Henri Beaufay, Alain Amar-Costesec, Ernest Feytmans, Denise Thinès-Sempoux, Maurice Wibo, Mariette Robbi, Jacques Berthet; ANALYTICAL STUDY OF MICROSOMES AND ISOLATED SUBCELLULAR MEMBRANES FROM RAT LIVER : I. Biochemical Methods . J Cell Biol 1 April 1974; 61 (1): 188–200. doi: https://doi.org/10.1083/jcb.61.1.188
Download citation file: