Liver homogenates have been submitted to quantitative fractionation by differential centrifugation. Three particulate fractions: N (nuclear), ML (large granules), and P (microsomes), and a final supernate (S) have been obtained. The biochemical composition of the microsomal fraction has been established from the assay and distribution pattern of 25 enzymatic and chemical constituents. These included marker enzymes for mitochondria (cytochrome oxidase), lysosomes (acid phosphatase and N-acetyl-ß-glucosaminidase), and peroxisomes (catalase). The microsomal preparations were characterized by a moderate contamination with large cytoplasmic granules (only 6.2% of microsomal protein) and by a high yield in microsomal components. Enzymes such as glucose 6-phosphatase, nucleoside diphosphatase, esterase, glucuronyltransferase, NADPH cytochrome c reductase, aminopyrine demethylase, and galactosyltransferase were recovered in the microsomes to the extent of 70% or more. Another typical behavior was shown by 5'-nucleotidase, alkaline phosphatase, alkaline phosphodiesterase I, and cholesterol, which exhibited a "nucleomicrosomal" distribution. Other complex distributions were obtained for several constituents recovered in significant amount in the microsomes and in the ML or in the S fraction.
Article|
April 01 1974
ANALYTICAL STUDY OF MICROSOMES AND ISOLATED SUBCELLULAR MEMBRANES FROM RAT LIVER : II. Preparation and Composition of the Microsomal Fraction
Alain Amar-Costesec,
Alain Amar-Costesec
From the Laboratoire de Chimie Physiologique, Université de Louvain, Louvain, Belgium
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Henri Beaufay,
Henri Beaufay
From the Laboratoire de Chimie Physiologique, Université de Louvain, Louvain, Belgium
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Maurice Wibo,
Maurice Wibo
From the Laboratoire de Chimie Physiologique, Université de Louvain, Louvain, Belgium
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Denise Thinès-Sempoux,
Denise Thinès-Sempoux
From the Laboratoire de Chimie Physiologique, Université de Louvain, Louvain, Belgium
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Ernest Feytmans,
Ernest Feytmans
From the Laboratoire de Chimie Physiologique, Université de Louvain, Louvain, Belgium
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Mariette Robbi,
Mariette Robbi
From the Laboratoire de Chimie Physiologique, Université de Louvain, Louvain, Belgium
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Jacques Berthet
Jacques Berthet
From the Laboratoire de Chimie Physiologique, Université de Louvain, Louvain, Belgium
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Alain Amar-Costesec
From the Laboratoire de Chimie Physiologique, Université de Louvain, Louvain, Belgium
Henri Beaufay
From the Laboratoire de Chimie Physiologique, Université de Louvain, Louvain, Belgium
Maurice Wibo
From the Laboratoire de Chimie Physiologique, Université de Louvain, Louvain, Belgium
Denise Thinès-Sempoux
From the Laboratoire de Chimie Physiologique, Université de Louvain, Louvain, Belgium
Ernest Feytmans
From the Laboratoire de Chimie Physiologique, Université de Louvain, Louvain, Belgium
Mariette Robbi
From the Laboratoire de Chimie Physiologique, Université de Louvain, Louvain, Belgium
Jacques Berthet
From the Laboratoire de Chimie Physiologique, Université de Louvain, Louvain, Belgium
Received:
August 08 1973
Online Issn: 1540-8140
Print Issn: 0021-9525
Copyright © 1974 by The Rockefeller University Press
1974
J Cell Biol (1974) 61 (1): 201–212.
Article history
Received:
August 08 1973
Citation
Alain Amar-Costesec, Henri Beaufay, Maurice Wibo, Denise Thinès-Sempoux, Ernest Feytmans, Mariette Robbi, Jacques Berthet; ANALYTICAL STUDY OF MICROSOMES AND ISOLATED SUBCELLULAR MEMBRANES FROM RAT LIVER : II. Preparation and Composition of the Microsomal Fraction . J Cell Biol 1 April 1974; 61 (1): 201–212. doi: https://doi.org/10.1083/jcb.61.1.201
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