Treatment of Nitella antheridia with colchicine results in various sperm abnormalities, depending upon duration of exposure and subsequent recovery. Early effects of treatment include disappearance of spindle fibers and a cessation of ordered cell wall formation in dividing cells. Sperm released from antheridia treated for 24 hr and allowed to recover for 4–5 days possess branched flagella. After a recovery period of 6–10 days the sperm appear normal; however, following longer recovery periods, the sperm exhibit variations in size and number of flagella. Branched flagella contain a variety of microtubule patterns ranging from branches containing a single microtubule to flagella with an excess of microtubules. Spermatids which differentiate in the presence of colchicine lack flagella and a microtubular sheath. Nuclear contents undergo condensation stages; however, the nucleus as a whole does not undergo the orderly elongation and coiling characteristic of untreated Nitella spermatids. Long-term colchicine treatment followed by a recovery period produces atypical microtubules and microtubular aggregations in the spermatid. The results indicate that colchicine affects not only polymerization of microtubule subunits but also factors responsible for their ordered spatial relationships in the cell. The presence of microtubules is a prerequisite for normal morphological changes during spermiogenesis.
Article| August 01 1970
THE EFFECTS OF COLCHICINE ON SPERMATOGENESIS IN NITELLA
F. Rudolf Turner
From the Cell Research Institute, The University of Texas at Austin, Austin, Texas 78712.
Dr. Turner's present address is Department of Botany, Indiana University, Bloomington, Indiana 47401
Received: November 18 1969
Revision Received: March 02 1970
Online Issn: 1540-8140
Print Issn: 0021-9525
Copyright © 1970 by The Rockefeller University Press
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F. Rudolf Turner; THE EFFECTS OF COLCHICINE ON SPERMATOGENESIS IN NITELLA . J Cell Biol 1 August 1970; 46 (2): 220–234. doi: https://doi.org/10.1083/jcb.46.2.220
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