The fine structure of the nuclei of logarithmically growing Tetrahymena pyriformis, strain HSM, was studied at 30-minute intervals throughout the cell cycle. Organisms were selected at similar stages of cytokinesis by means of a braking pipette, incubated, fixed in OsO4, and embedded in agar to facilitate subsequent preparation for electron microscopy. Aggregates of micronuclear chromatin underwent a decrease in density and number with a concomitant increase in size throughout interphase. There were no impressive changes in macronuclear morphology. It was found possible to estimate a cell's progress through interphase by observation of micronuclear morphology, but attempts to correlate changes in fine structure with periods of DNA synthesis were unsuccessful.
Article| December 01 1965
THE FINE STRUCTURE OF THE NUCLEI OF TETRAHYMENA PYRIFORMIS THROUGHOUT THE CELL CYCLE
Charles J. Flickinger
From the Department of Anatomy, University of Colorado Medical Center, Denver, Colorado.
The author's present address is Department of Anatomy, Harvard Medical School, Boston, Massachusetts
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Charles J. Flickinger; THE FINE STRUCTURE OF THE NUCLEI OF TETRAHYMENA PYRIFORMIS THROUGHOUT THE CELL CYCLE . J Cell Biol 1 December 1965; 27 (3): 519–529. doi: https://doi.org/10.1083/jcb.27.3.519
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