This report describes a rapid, efficient method for isolating macronuclei from Tetrahymena. The macronuclear fraction contains only small amounts of micronuclear material and little detectable whole cell or cytoplasmic contamination. A method is also described for preparing a "micronuclear fraction" which contains 20–40 micronuclei for every macronucleus present. Electron microscope observations indicate that the ultrastructure of the nuclei in the macronuclear fraction closely resembles that of nuclei in situ. The presence of ribosomes on the outer membrane of micronuclei and of pores in the micronuclear envelope is also described.
Article| December 01 1970
STUDIES ON NUCLEAR STRUCTURE AND FUNCTION IN TETRAHYMENA PYRIFORMIS : II. Isolation of Macro- and Micronuclei
Martin A. Gorovsky
From the Whitman Laboratory, the Department of Biology, The University of Chicago, Chicago, Illinois 60637.
Dr. Gorovsky's present address is the Department of Biology, The University of Rochester, Rochester, New York 14627
Received: March 04 1970
Revision Received: July 28 1970
Online Issn: 1540-8140
Print Issn: 0021-9525
Copyright © 1970 by The Rockefeller University Press
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Martin A. Gorovsky; STUDIES ON NUCLEAR STRUCTURE AND FUNCTION IN TETRAHYMENA PYRIFORMIS : II. Isolation of Macro- and Micronuclei . J Cell Biol 1 December 1970; 47 (3): 619–630. doi: https://doi.org/10.1083/jcb.47.3.619
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