Gaudin et al. identify a type of kinesin that ferries HIV to the plasma membrane, helping the virus escape from macrophages.
HIV reproduces inside T cells, killing them in the process. But the virus can also replicate in macrophages, which survive infection and serve as reservoirs of HIV. In T lymphocytes, new viral particles are born at the plasma membrane, but in macrophages the viruses assemble inside cytoplasmic containers called virus-containing compartments (VCCs). To break out of a macrophage, a virus particle therefore has to travel to the plasma membrane.
Gaudin et al. showed that the virus hitches a ride with the microtubule-based kinesin motor KIF3A. The researchers found that knocking down KIF3A dramatically reduced the release of HIV particles from macrophages. Yet the procedure did not have any effect on the amount of HIV escaping from T cells.
KIF3A drives HIV along microtubules, the researchers discovered. For example, the localization of KIF3A-positive VCCs depended on microtubules. Gaudin et al. also observed KIF3A proteins and VCCs moving in tandem along the filaments. VCCs build up in cells lacking KIF3A, suggesting that their movements stall in the absence of the kinesin. How HIV exits VCCs remains uncertain, but the results indicate that inhibiting KIF3A might offer a new way to combat the virus.
