Spinocerebellar ataxia type 5 (SCA5) is an autosomal dominant neurodegenerative disorder caused by mutations in the SPTBN2 gene encoding β-III–spectrin. To investigate the molecular basis of SCA5, we established a series of transgenic Drosophila models that express human β-III–spectrin or fly β-spectrin proteins containing SCA5 mutations. Expression of the SCA5 mutant spectrin in the eye causes a progressive neurodegenerative phenotype, and expression in larval neurons results in posterior paralysis, reduced synaptic terminal growth, and axonal transport deficits. These phenotypes are genetically enhanced by both dynein and dynactin loss-of-function mutations. In summary, we demonstrate that SCA5 mutant spectrin causes adult-onset neurodegeneration in the fly eye and disrupts fundamental intracellular transport processes that are likely to contribute to this progressive neurodegenerative disease.
Spinocerebellar ataxia type 5 (SCA5) is an autosomal dominant neurodegenerative disease that primarily affects the cerebellum. Affected patients have progressive cerebellar cortical atrophy and profound Purkinje cell loss. Similar clinical presentations reported in three different SCA5 families include upper and lower limb incoordination, slurred speech, and eye movement abnormalities. Age of onset typically occurs during the third or fourth decade of life, and symptoms worsen over time (Liquori et al., 2002). SCA5 is caused by mutations in the SPTBN2 gene, which encodes β-III–spectrin, a cytoskeletal protein highly expressed in Purkinje cells (Ikeda et al., 2006). An American and a French SCA5 family have distinct, nonoverlapping in-frame deletions in the third of the 17 spectrin repeats, and both of these deletions are predicted to disrupt the triple α-helical structure of the spectrin repeat and the conformation of the spectrin tetramer. A third reported SCA5 family, from Germany, has a missense mutation in the second calponin homology domain. This region of the protein has been reported to bind actin and the ARP1 subunit of dynactin, providing a link between the actin cytoskeletal network and motor proteins (Holleran et al., 2001).
Although it is not yet clear how β-III–spectrin mutations cause Purkinje cell death in SCA5 patients, several lines of evidence have led to the proposal that SCA5 pathogenesis could result from the destabilization of specialized synaptic membrane domains and/or defects in intracellular transport. First, wild-type but not mutant β-III–spectrin stabilizes the Purkinje cell–specific excitatory amino acid transporter 4 (EAAT4) at the surface of the plasma membrane (Ikeda et al., 2006). In addition, cell fractionation studies have shown differences in the localization of EAAT4 and the glutamate receptor delta 2 subunit (GluRδ2) in cerebellar synaptosomal fractions from SCA5 versus control autopsy tissue (Ikeda et al., 2006), and the C-terminal domains of GluRδ2 and EAAT4 have been shown to physically interact with spectrin (Hirai and Matsuda, 1999; Jackson et al., 2001). Finally, β-III–spectrin is a Golgi- and vesicle-associated protein that interacts with dynactin (Holleran et al., 2001). This interaction is thought to be required for proper cargo attachment and motor activity (Muresan et al., 2001). Although data from in vitro biochemical experiments support a role for β-III–spectrin in intracellular transport, in vivo models are needed to test if SCA5 mutations cause neuronal transport deficits.
Because Drosophila has proven to be an excellent organism to model basic cellular defects of human neurodegenerative disease (for review see Bilen and Bonini, 2005), we have developed a Drosophila model to investigate the molecular mechanisms of SCA5. The fly genome contains one α-spectrin, one conventional β-spectrin, and one heavy spectrin (βH-spectrin) gene, each of which is highly expressed at both central and peripheral synapses. Fly β-spectrin shares 50% amino acid homology with human β-III–spectrin, as well as conservation of all functional domains, including each of the regions containing the human mutations (Fig. S1 A, I and II).
Similar to human β-III–spectrin, fly β-spectrin has also been implicated in membrane stabilization and intracellular transport functions. Loss or reduced expression of fly β-spectrin in neurons results in severe defects in the formation and stabilization of synaptic junctions (Pielage et al., 2005, 2006). Segmental axons from larvae in which expression of β-spectrin has been ubiquitously abolished or conditionally eliminated in neurons show aberrant distribution of synaptic proteins, which accumulate within axonal swellings (Featherstone et al., 2001; Pielage et al., 2005, 2006).
Here, we show that expression of SCA5 mutant, but not wild-type, β-spectrin proteins causes neurodegeneration in the fly eye and deficits in synapse formation at the neuromuscular junction (NMJ). Additionally, we present live imaging and genetic evidence that the SCA5 mutations disrupt intracellular transport, a basic cell biological process also implicated in several other neurodegenerative diseases including amyotrophic lateral sclerosis and Huntington’s disease (De Vos et al., 2008).
Expression of human SCA5 mutant β-III–spectrins cause dosage-dependent, progressive neurodegeneration in the
To understand the molecular mechanisms of SCA5, we cloned the full-length myc-tagged human SPTBN2 cDNA carrying the wild-type sequence (human spectrin wild type [hSPWT]), mutations found in an American family descended from President Abraham Lincoln’s paternal grandparents (human spectrin American mutation [hSPAM]; Ikeda et al., 2006), or a German family (human spectrin German mutation [hSPGM]; Ikeda et al., 2006) into the transformation vector pUASp (Fig. S1 B) and performed embryo injections. The resulting transgenic flies were crossed to the eye-specific gmr-GAL4 driver (Freeman, 1996) to ectopically express the human transgenes in the eye. Expression of human β-III–spectrin with the American family mutation (gmr-GAL4/hSPAM) results in a severe rough eye phenotype characterized by disorganized ommatidia and loss of mechanosensory bristles (Fig. 1 A, II), whereas expression of the German mutant spectrin (gmr-GAL4/+; hSPGM/+) causes a milder eye phenotype (Fig. 1 A, III). In contrast, expression of wild-type human β-III–spectrin (gmr-GAL4/+; hSPWT/+) causes only a very mild ommatidial phenotype that does not disrupt normal eye development (Fig. 1 A, IV) and is similar to the control driver flies (gmr-GAL4/+; Fig. 1 A, I). Protein analysis shows that flies expressing wild-type (gmr-GAL4/+; hSPWT/+) and the American mutant spectrin (gmr-GAL4/hSPAM) produce comparable levels of transgenic protein, whereas flies expressing the German mutant spectrin (gmr-GAL4/+; hSPGM/+) have significantly lower β-III–spectrin expression (Fig. 1 B; P = 0.025, n = 4). As expected the phenotypes are dosage dependent; flies carrying two copies of the hSPGM transgene (gmr-GAL4/+; hSPGM/hSPGM) express higher levels of β-III–spectrin compared with the heterozygous flies (Fig. 1 B) and develop a stronger rough eye phenotype (Fig. 1 A, V).
To determine if the SCA5 spectrin mutations affect retinal neurons, histological analysis was performed. Sections taken through the eye of 10-d-old flies showed a severe disruption of the retinal organization, with thinning of the retina and loss of retinal neurons in flies expressing the American and German SCA5 mutations (Fig. 1, E, E′, F, and F′, see arrows) compared with the gmr-GAL4 driver line (Fig. 1, C and C′) or flies expressing wild-type β-III–spectrin (Fig. 1, D and D′). Analysis of flies expressing the SCA5 mutations revealed that the extent of neurodegeneration in the eye became more severe as flies aged. By day 30, eyes from flies expressing the American SCA5 mutation showed dramatic changes in pigmentation and abundant necrotic tissue (Fig. 1, I–I″). Similarly, heterozygous flies expressing low levels of the German SCA5 mutation developed eye pigmentation abnormalities by day 30 (Fig. 1, J–J″), whereas homozygous flies expressing higher levels of German mutant spectrin showed dramatic changes in eye pigmentation by day two, and patches of severe necrosis by day 10 (Fig. 1, K and K′). In contrast, no changes in external eye morphology or pigmentation were found in control flies expressing wild-type β-III–spectrin (Fig. 1, H and H″). Taken together, these results demonstrate that expression of human β-III–spectrin containing either the American or German mutations causes a progressive, dominant neurodegenerative phenotype.
The basic structural unit of the spectrin network is a dimer formed by direct association between α- and β-spectrin subunits (for review see Bennett and Baines, 2001). To begin to understand the molecular basis for the observed neurodegenerative phenotypes, we tested whether human β-III–spectrin assembles into the fly α/β-spectrin complex by immunoprecipitation (Fig. 1 L). Proteins from flies expressing wild-type human β-III–spectrin in the eye were immunoprecipitated with anti-fly α-spectrin antibody, and protein blot analysis shows that human β-III–spectrin and fly α-spectrin interact. This result indicates that the human β-III–spectrin does appear to incorporate into the α/β-spectrin complexes in flies.
Human β-III–spectrin and fly β-spectrin share some binding partners and functional pathways
To further characterize the effects of the SCA5 mutations, we developed a second set of transgenic flies in which the human SCA5 mutations were incorporated into Drosophila cDNA constructs designed to overexpress wild-type (fly spectrin wild type [FSPWT]) and mutant fly β-spectrins (fly spectrin American mutant [FSPAM] and German mutant [FSPGM]; Fig. S1 C). Analysis of protein expression indicates that transgenic lines for each of the genotypes in this study have comparable and approximately twofold higher levels of fly β-spectrin than the endogenous spectrin detected in the gmr-GAL driver line (Fig. 2 B; P = 0.18, n = 4).
Similar to the human mutant β-III–spectrin transgenes, the expression of the fly β-spectrin transgenes containing either the American (FSPAM) or German (FSPGM) mutation also produced strong degenerative eye phenotypes (Fig. 2 A, II and III). In contrast, animals overexpressing wild-type fly β-spectrin do not develop eye degeneration and only show mild ommatidial disorganization comparable to gmr-GAL4/+ flies (gmr-GAL4/FSPWT; Fig. 2 A, I). These data suggest that human β-III and fly β-spectrin act in at least some of the same functional pathways when expressed in the fly and support the use of these models to study dominant or dominant-negative effects of the SCA5 mutations.
Mutant β-spectrin impairs synaptic terminal growth at the NMJ
We additionally explored the effects of the SCA5 mutations by expressing the human and fly β-spectrin transgenes in all neurons using the elav-GAL4 driver, and examined synaptogenesis at the NMJ in third instar larvae (Fig. 3, A–G). Quantitative results are reported as relative synapse size (bouton number per muscle area; see Materials and methods) to account for differences in bouton number caused by variations in muscle size during normal growth. We observed reduced bouton number in larvae expressing mutant hSPAM, FSPAM, and FSPGM but not the wild-type (hSPWT and FSPWT) transgenes (P < 0.0001; Fig. 3). Although, under the same conditions, we did not detect differences in bouton counts in larvae expressing the hSPGM transgene (Fig. 3 C), this result is likely explained by the relatively low expression in this line (Fig. 1 B).
To further characterize the synaptic phenotype, we examined the organization of several proteins that are known to contribute to the spectrin-mediated development and stability of the NMJ (Pielage et al., 2006, 2008; Koch et al., 2008). In wild-type animals, the transynaptic cell adhesion molecule fasciclin II (Fas II) is organized in a honeycomb-like distribution that surrounds presynaptic active zones. In contrast, SCA5 mutant larvae have defects in the organization and morphology of synaptic boutons and in the distribution of Fas II throughout the synapse (Fig. S2) that are similar to the phenotypes described in β-spectrin RNAi lines. In addition, we show that the expression of mutant SCA5 transgenes does not result in synapse retraction, in contrast to results described for flies lacking β-spectrin expression at the NMJ (Pielage et al., 2005, 2006). Despite the reduction in the number of synaptic boutons observed in SCA5 mutant animals, most presynaptic terminals were found to reside in opposition to postsynaptic membranes, as indicated by costaining with the synaptic markers synaptotagmin (syt) and dics large (Dlg; Fig. S3, A–I). Our studies also show that mutant spectrin does not impair the localization of ankyrin at synaptic sites (Fig. S3, J–R) or affect microtubule organization within the axon and NMJ (Fig. S4). Taken together, these data demonstrate that the phenotypes induced by overexpression of mutant human and fly β-spectrin overlap, but are distinct from phenotypes previously reported by RNAi depletion of β-spectrin (Pielage et al., 2005).
Mutant β-spectrin expression impairs larval locomotion
The reduced synaptic growth observed in animals expressing mutant spectrins suggests the possibility that intracellular transport deficits contribute to the structural abnormalities at the synapse. To test this hypothesis, we examined larvae expressing the American and German SCA5 mutations for the posterior paralysis or “tail-flip” phenotype previously described for mutations that cause aberrant axonal transport (Hurd and Saxton, 1996; Martin et al., 1999; Bowman et al., 2000; Haghnia et al., 2007). Larvae expressing mutant but not wild-type fly β-spectrin in neurons exhibit the tail flip crawling phenotype due to paralysis of the posterior segments of the larval body (Fig. 4, A–C; and Videos 1–3). Although larvae expressing one copy of the human β-III–spectrin transgenes (elav-GAL4/+; hSPAM/+ and elav-GAL4/+; hSPGM/+) did not develop similar motor defects, this likely reflects the lower levels of human protein expression compared with fly β-spectrin. Consistent with this idea, animals expressing two copies of the American mutant spectrin transgene (elav-GAL4/+; hSPAM/hSPAM) produce higher levels of mutant spectrin protein and exhibit the tail flip phenotype in ∼31% of the larvae (10/32 larvae; Video 4). By comparison, control animals expressing two wild-type copies of the spectrin transgene (elav-GAL4/+; hSPWT/hSPWT) at comparable protein levels exhibit a tail phenotype in only 5% of the larvae (2/40 larvae). In addition, if one copy of the endogenous β-spectrin gene is silenced by mutation, then a single copy of the hSPAM mutant transgene produces posterior paralysis in ∼32% (12/38) of larvae. In the control cross, a single copy of the wild-type human spectrin transgene (hSPWT) produced paralysis in <5% of the larvae (2/41). Finally, we show that RNAi depletion of the endogenous β-spectrin produces paralysis in ∼88% (29/33) of larvae, and that the introduction of two copies of the hSPWT transgene rescues the paralysis in ∼90% of the larvae (4/38 paralyzed). In summary, the dosage dependence of the hSPAM larval paralysis phenotype suggests that human and fly mutant spectrins interfere with the normal functions of wild-type β-spectrin, and that these dominant-negative effects are likely to contribute to disease.
Mutant spectrin affects both the anterograde and retrograde transport of synaptic vesicles
To determine if the larval tail paralysis and synaptogenesis phenotypes could be explained by defects in axonal transport, we analyzed the distribution of GFP-tagged synaptic vesicle membrane protein syt-GFP. In larvae expressing wild-type β-spectrin (elav-GAL4–syt-GFP/+; FSPWT/+), syt was evenly distributed as small puncta (Fig. 4 D). In contrast, larvae expressing mutant spectrins (elav-GAL4–syt-GFP/+; FSPAM/+ and elav-GAL4–syt-GFP/+; FSPGM/+) showed an accumulation of syt-GFP–positive vesicles within axonal aggregates (Fig. 4, E and F, see arrows). Considering that segmental axons have a mean diameter of ∼0.5 µm (Rusu et al., 2007), we next quantified the number of large syt accumulations (diameter ≥ 0.7 µm), which are likely to cause axonal swellings and disrupt the normal trafficking of intracellular cargoes (Gunawardena and Goldstein, 2001). We found a significant difference (Fig. 4 G; ***, P < 0.0001) in the frequency of axonal jams in larvae expressing mutant FSPAM and FSPGM transgenes (Fig. 4 G) compared with wild-type controls. In addition, we show that the distributions of the synaptic vesicle protein, cysteine string protein (CSP; Fig. 4 H), as well as the dynein motor protein (Fig. 4 I), overlap with sites of syt-GFP axonal aggregates (Fig. 4, H and I).
To determine the effects of the SCA5 mutations on synaptic vesicle transport, we imaged and tracked the dynamic behavior of vesicles in live segmental axons. In flies overexpressing wild-type β-spectrin, a large number of syt-GFP–containing vesicles underwent rapid unidirectional movements. Kymograph analysis shows that this motion is characterized by long unidirectional runs (Fig. 4 J, I and II, diagonal line) interrupted by pauses and/or infrequent short reversals in the direction of vesicle transport (Fig. 4 J, I and II; and Video 5). In contrast, in segmental axons expressing either the American or German SCA5 mutations, the unidirectional bias in vesicle movement was reduced. Syt-GFP vesicles exhibited frequent reversals in the direction of transport (Fig. 4 J, zigzag line), traveling for much shorter distances in any one direction. Representative kymographs and videos that illustrate the behavior of synaptic vesicles in mutant β-spectrin axons are shown in Fig. 4 J (III and IV) and Video 6. In addition, quantitative analysis confirmed that syt-GFP vesicles moved significantly slower in mutant compared with wild-type axons (Table I). Competition by the mutant spectrins may alter the dynamics of motor attachment to vesicular cargo and/or interfere with the binding of regulatory partners that influence motor activity.
To assess whether mutant β-spectrin affects anterograde, retrograde, or both types of axonal transport, mutant spectrin and the vesicle marker neuronal synaptobrevin-GFP (n-syb-GFP) were expressed using the motor neuron–specific D42-GAL4 driver. In control larvae (D42-GAL4–n-syb-GFP/FSPWT), most fluorescent particles were moving in long runs (Video 7). In contrast, animals expressing either of the mutant spectrins (D42-GAL4–n-syb-GFP/FSPAM and D42-GAL4–n-syb-GFP; FSPGM) showed numerous stationary n-syb-GFP particles, some of which were trapped within axonal swellings (Video 8). Analysis of mean velocity and run length for anterograde and retrograde movements indicate that mutant spectrin disrupts transport in both directions (Table II), and that the GFP vesicles that retained motility had slower velocities and traveled shorter distances than in controls.
Dynein–dynactin deficits genetically enhance spectrin-induced phenotypes
The rough eye and transport phenotypes caused by the mutant spectrins are similar to phenotypes previously described in dynein and dynactin mutants (McGrail et al., 1995; Martin et al., 1999; Boylan et al., 2000). If these phenotypes result from the failure of spectrin to properly engage in retrograde transport, then we would predict that dynein, and/or dynactin mutations, would enhance the mutant spectrin phenotypes. To test this hypothesis, flies carrying a hypomorphic dynein heavy chain allele (DHC64C6-10) were crossed to flies containing a single copy of the hSPAM transgene, and progeny were scored for posterior paralysis. In contrast to single mutant animals, which do not show posterior paralysis, larvae expressing both spectrin and dynein mutations (elav-GAL4–syt-GFP/+; hSPAM/+; DHC64C6-10/+) develop the tail flip (Fig. 5 A, III) and axonal vesicle accumulation phenotypes (Fig. 5 B, III). Additionally, double mutant larvae display significant reductions in synapse size at the NMJ compared with single mutant controls as well as hSPWT and elav-GAL4 animals (Fig. 5 C; ***, P < 0.0001). A similar genetic interaction occurs between flies expressing the American mutation and the dominant Gl1 mutation in the p150Glued subunit of dynactin. We observed larval paralysis in 44% (22/50) of progeny carrying the American spectrin transgene in a p150Glued mutant background (elav-GAL4–syt-GFP/+; hSPAM/+; Gl1/+), whereas only 5% (5/50) of sibling progeny expressing the mutant spectrin transgene in the absence of the Gl1 (elav-GAL4–syt-GFP/+; hSPAM/+; Tb/+) exhibited larval paralysis. Sibling progeny (hSPAM/+; Gl1/+) that lack the elav-GAL4 driver showed the tail paralysis phenotype in 18% (9/50) of larvae. Additionally, the rough eye phenotypes caused by the expression of the SCA5 mutant spectrin are enhanced by dynein and dynactin mutations. This analysis was facilitated by the generation of recombinant lines that carry both the GAL4 driver and the mutant spectrin transgenes (gmr-GAL4-hSPAM or gmr-GAL4-FSPGM) on a single chromosome (Fig. 5 D). The recombinant chromosomes, which have reduced levels of transgene expression (Fig. S5 A) develop a less severe eye phenotype (Fig. 5 D), which allowed genetically enhanced phenotypes to be more easily detected. Adult flies with one copy of the American or German mutant spectrin transgenes, which are also heterozygous for a hypomorphic mutant dynein allele, DHC64C6-10, exhibit severe eye phenotypes involving disruptions of the ommatidial hexagonal packing and loss of interommatidial bristles (gmr-GAL4-hSPAM/+; DHC64C6-10/+ and gmr-GAL4-FSPGM/+; DHC64C6-10/+; Fig. 5, G and H). In contrast, fly strains carrying only the mutant spectrin transgene or the dynein mutation exhibit only mild or wild-type eye phenotypes (Fig. 5, D–F). Similarly, the dominant Gl1 dynactin mutation, which by itself causes disorganization of the ommatidia (Fig. 5 I), genetically enhances the phenotypes of flies expressing either the American or German spectrin mutations (gmr-GAL4-hSPAM/+; Gl1/+ and gmr-GAL4-FSPGM/+; Gl1/+; Fig. 5, J and K). We excluded the possibility that these genetic interactions simply result from alterations in spectrin expression by protein blotting (Fig. S5, B and C). The genetic interactions observed are consistent with the functional intersection of the dynein and spectrin pathways.
We have established a series of transgenic Drosophila models to investigate the molecular mechanisms of spinocerebellar ataxia type 5. Toward these goals, we show that expression of the American family and German SCA5 mutations in flies causes neurodegenerative changes in the adult eye as well as motor coordination, synaptogenesis, and axonal transport deficits in Drosophila larvae. These phenotypes are genetically enhanced by partial loss-of-function mutations in dynein or a dominant-negative mutation in the p150Glued component of dynactin. Taken together, these data demonstrate that SCA5 mutations can trigger adult-onset neurodegeneration in the fly eye and affect fundamental intracellular transport functions that are likely to play a role in this progressive neurodegenerative disease.
SCA5 mutations cause dominant dosage-dependent phenotypes
In Drosophila, previous work has established that the single α- and β-spectrin genes are essential (Dubreuil et al., 2000). In contrast, in humans there are four β-spectrin genes, and mutations in one copy of the β-III–spectrin gene, which is highly expressed in Purkinje cells, lead to a dominantly inherited form of ataxia (Ikeda et al., 2006). We have used ectopic expression of human β-III–spectrin and overexpression of endogenous fly β-spectrin to define the basic biological processes affected by the SCA5 mutations and to better understand the mechanisms of neurodegeneration. Our observations suggest that the human SCA5 mutations cause dominant-negative effects. Consistent with this hypothesis are data showing that the severity of the eye and larval phenotypes found in flies expressing mutant β-spectrin increases when the levels of endogenous wild-type spectrin are reduced. Our observations do not exclude the possibility that the SCA5 mutations may also cause abnormalities in protein stability and conformation that result in additional dominant gain-of-function effects. Future studies will be needed to evaluate the relative contributions of these mechanisms to the neurodegenerative Purkinje cell phenotypes seen in SCA5 patients.
The position of the German mutation in the second calponin homology domain suggests that the mutant protein disrupts the normal interactions of the wild-type protein with the actin cytoskeleton and/or the ARP1 subunit of dynactin (Holleran et al., 2001). Consistent with these data, both the German and the American mutations cause intracellular transport deficits that are similar to the phenotypes of mutant Arp1 flies (Haghnia et al., 2007). In the case of the American family, the deletion within the third spectrin repeat may result in similar effects on dynactin pathways by disrupting protein–protein interactions that depend on the helical conformation of the spectrin repeats, and the formation of stable α-β–spectrin tetramers. The similar phenotypes found in flies expressing the SCA5 mutations in the context of either the human or fly β-spectrin proteins indicate that many of the binding partners and functional roles of spectrin are conserved between humans and flies. Indeed, antibodies specific for Drosophila α-spectrin can immunoprecipitate human β-III–spectrin from protein extracts (Fig. 1 L), and the human β-III–spectrin can rescue the larval tail paralysis induced after RNAi depletion of fly β-spectrin.
In Drosophila, the single β-spectrin gene product is the orthologue of vertebrate β-spectrins 1, 2, 3, and 4. Thus, our studies suggest that all vertebrate β-spectrins are likely capable of post-Golgi transport while potentially targeted to specific subsets of vesicular cargoes. As previously suggested (Bennett and Baines, 2001), it is unlikely that human β-III–spectrin is a general Golgi-specific protein, as axonal transport involves the trafficking of post-Golgi structures (Horton and Ehlers, 2003; Ye et al., 2007). Moreover, the expression of human β-III–spectrin is highly enriched in the cerebellum and nervous system (Sakaguchi et al., 1998), a distribution that is consistent with the localized atrophy of the cerebellar hemispheres and the neurological symptoms of SCA5 patients.
Drosophila model for SCA5
Overexpression of wild-type and mutant spectrin in Drosophila neurons allows us to investigate the pathways that contribute to spectrin function and regulation, and should provide insight into the molecular basis of SCA5 pathology. Although a similar approach has been exploited to study a number of other human neurodegenerative diseases (e.g., Feany and Bender, 2000; Jinet al., 2003; Gunawardena et al., 2003; Jung and Bonini, 2007), this approach does have limitations. In contrast to the human disease, mutant spectrin is expressed in the presence of two copies of the endogenous wild-type fly β-spectrin gene. Although it would be interesting to compare the effects of the SCA5 mutations using a knock-in approach in which a single mutant and single wild-type β-spectrin allele are expressed, Drosophila has only a single, essential β-spectrin gene that is widely expressed in multiple tissues. This would limit the types of analyses that could be performed and complicate the interpretation of knock-in phenotypes. Moreover, such a knock-in fly model would not mimic the human situation in which four β-spectrin genes and a variety of α/β heterotetramers exhibit distinct functions and patterns of expression. Although no Drosophila model, including the one characterized in this study, can perfectly model a human disease, we demonstrate that the SCA5 mutant β-III–spectrins act within the normal spectrin pathway, competing with endogenous Drosophila β-spectrin to produce dosage-dependent phenotypes. We suggest that the phenotypes caused by overexpression of SCA5 mutant but not wild-type spectrin in our models provide insight into the molecular consequences of the mutations that are likely to be helpful in understanding the molecular mechanisms underlying the human disease.
SCA5 mutations cause synaptic deficits
The major roles of spectrin in strengthening cellular membranes and organizing ion channels, neurotransmitter receptors, cell adhesion molecules, and other components into specialized synaptic membrane domains are well established (Bloch and Morrow, 1989; Jenkins and Bennett, 2001; Lee and Discher, 2001; Komada and Soriano, 2002; Lacas-Gervais et al., 2004; Hammarlund et al., 2007; Bennett and Healy, 2008). In particular, recent work suggests that wild-type β-III–spectrin, but not SCA5 mutant spectrin, can stabilize the glutamate transporter EAAT4 at the cell membrane (Jackson et al., 2001; Ikeda et al., 2006).
Consistent with possible dominant-negative effects, expression of the SCA5 mutant proteins results in reduced numbers of synaptic boutons at synaptic termini. In Drosophila, spectrin function at the synapse has been extensively studied in motor neurons (Featherstone et al., 2001; Pielage et al., 2005, 2006). These studies first characterized pre- and postsynaptic spectrin localization and function during motor neuron synaptogenesis in Drosophila. Loss of presynaptic spectrin reportedly eliminates several essential cell adhesion molecules from synaptic sites and leads to defects in neurotransmission and the disassembly and elimination of synapses at the NMJ (Pielage et al., 2005, 2006). In addition, the RNAi depletion of presynaptic β-spectrin has been reported to disrupt microtubule organization at the terminal boutons, to generate large accumulations of synaptic vesicles with motor axons, and to reduce the accumulation of synaptic vesicles within the terminal boutons. These latter studies suggested a link between spectrin and vesicle transport either through a direct role in motor-based transport or an indirect affect on cytoskeletal organization. Although we observed similar defects in synaptic growth and trafficking in axons expressing mutant SCA5 spectrin, we also detected several distinct phenotypes. For example, synaptic retraction and loss of Fas II and Ank was reported when β-spectrin is depleted by RNAi (Pielage et al., 2005, 2006; Koch et al., 2008), but these phenotypes are not found in animals expressing SCA5 mutant spectrins. Additionally, we did not observe dramatic alterations in synaptic microtubule distribution as previously reported after depletion of presynaptic β-spectrin (Pielage et al., 2005).
To test the possible role that transport deficits play in these phenotypes, we performed a series of genetic interaction studies. We show that loss of synapses at the NMJ in SCA5 mutant flies is enhanced in animals heterozygous for dynein-heavy chain and p150Glued mutations. These genetic interactions suggest that spectrin, dynein, and dynactin interact in a complex that mediates synapse stabilization. Although the protein interactions that mediate synapse stability are not fully understood, one model is that synaptic components at the membrane are stabilized through spectrin and ankyrin linkages to the cytoskeleton (Pielage et al., 2006, 2008; Ayalon et al., 2008; Koch et al., 2008), and that the establishment of these connections is facilitated by dynein/dynactin recruitment of microtubules (Carminati and Stearns, 1997; Adames and Cooper, 2000; Ligon et al., 2001; Schuyler and Pellman, 2001; Dujardin and Vallee, 2002). The binding of ankyrin to the p62 subunit of dynactin, as well as the reported binding of ankyrin to microtubules, supports the hypothesis that spectrin and ankyrin may act together to stabilize the synaptic membrane through the dynactin/dynein-mediated capture of microtubules (Ayalon et al., 2008; Koch et al., 2008; Pielage et al., 2008). Consistent with this model, synapse disassembly has also been reported after disruption of dynactin function (Eaton et al., 2002).
SCA5 mutations disrupt vesicle transport
Our investigations provide the first direct in vivo visualization of spectrin’s involvement in vesicle transport in neurons, in contrast to previous studies in Drosophila that indirectly suggested a role for β-spectrin in vesicle transport based on the accumulation of axonal cargos in fixed preparations of spectrin mutants or of motor neurons depleted of spectrins by RNAi treatment (Featherstone et al., 2001; Pielage et al., 2005, 2006). Here, we use live imaging of larval axons to directly observe the defective transport of vesicles after β-spectrin depletion by RNAi treatment, or after expression of the SCA5 mutations that specifically disrupt spectrin function. In vertebrates, the initial studies suggesting a role for spectrin in intracellular transport have been controversial. In particular, whether vertebrate β-III–spectrin is associated with Golgi vesicles has been complicated by the cross-reactivity of β-III–spectrin antisera with the related protein, nesprin (Beck, et al., 1994; Stankewich et al., 1998; Gough et al., 2003). More recent biochemical and in vitro motility studies (Muresan et al., 2001; Holleran et al., 2001) suggest a model in which two Arp1-binding sites on β-III–spectrin mediate the linkage between the dynein motor and cytoplasmic vesicles. In vitro imaging of protein-free liposomes or axonal vesicles from dissociated squid axoplasm shows that spectrin binding to dynactin and vesicle movement can be inhibited by the addition of competing spectrin peptides. In our studies, we show that SCA5 mutations in β-III–spectrin disturb the retrograde and anterograde transport in axons. Velocities and run lengths of vesicles in both directions are significantly reduced, and an elevated frequency of reversals is seen. The loss of unidirectional transport may indicate a decreased affinity in the attachment of motors to vesicular cargoes and/or increased competition and switching between oppositely directed motor proteins. It will be important to understand whether compromising spectrin’s function directly influences the binding of motor proteins or, alternatively, alters the binding of other factors that regulate motor activities. Although the axonal accumulations, or swellings, do not completely block the passage of moving cargoes, as synaptic vesicle proteins (e.g., syt, CSP, and syb) are transported and localized at synapses, the velocities of transport are reduced and, together with accumulation of intracellular cargos, may contribute to neurodegeneration.
Taken together, our data suggest that SCA5 is part of a growing group of diseases recently shown to involve the disruption of intracellular transport (for reviews see Chevalier-Larsen and Holzbaur, 2006; Duncan and Goldstein, 2006; De Vos et al., 2008). The fact that dynein and dynactin mutations enhance the spectrin mutant phenotypes suggests that mutant β-spectrin perturbs spectrin–dynactin interactions and affects binding of cargo to dynein (Holleran et al., 2001). Additionally, our results indicate that mutant spectrins do not only disrupt dynein-mediated retrograde transport, but also affect anterograde transport. Most likely, this reflects the interdependence between anterograde and retrograde transport that has been reported in multiple systems (Schroer et al., 1988; Brady et al., 1990; Waterman-Storer et al., 1997; Martin et al., 1999; Gross et al., 2000, 2002a,b; Pilling et al., 2006). In addition, an increasing number of biochemical and functional analyses suggest that dynactin acts as a switch to coordinate plus- and minus-end directed motor activities (Deacon et al., 2003; Gross, 2003; Haghnia et al., 2007). Our results do not exclude the possibility that the SCA5 mutations could affect kinesin-based transport (Hirokawa, 1998; De Matteis and Morrow, 2000; Takeda et al., 2000; Paik et al., 2004), even though no direct association between β-spectrin and kinesin-1 has been reported in Drosophila, and SCA5 phenotypes are not enhanced by kinesin mutations.
The spectrin–dynactin–dynein linkage in neurodegeneration and SCA5
Building on spectrin’s ability to stabilize protein domains at the membrane, one hypothesis is that spectrin also mediates the direct association of dynein and dynactin with proteins in membrane-bound cytoplasmic vesicles (Presley et al., 1997; Holleran et al., 2001; Muresan et al., 2001). We suggest that both synapse loss and vesicle transport defects caused by the SCA5 mutations may result from disruptions in the spectrin–dynactin–dynein adapter complex that links both vesicle and synaptic membranes to microtubules. The resultant neurodegeneration likely results from defects in both activities of the spectrin–dynactin–dynein linker adapter complex. At the synaptic membrane, the spectrin adapter recruits dynactin and dynein to the plasma membrane, promoting the capture of underlying cortical microtubules and stabilization of the synaptic membrane. Phenotypes of synapse disassembly are observed when fly β-spectrin expression is reduced or dynactin is disrupted (Eaton et al., 2002; Pielage et al., 2006). The synaptic deficits caused by the human SCA5 mutations may affect similar pathways by disrupting membrane–microtubule linkages that could lead to synapse disassembly. On synaptic vesicles, the spectrin adapter links the dynactin/dynein motor complex to the membrane, providing for transport of vesicles along the axonal microtubules. In our studies, we show that SCA5 mutations inhibit the transport of vesicles both to and from the synapse in axons. Similarly, vesicle transport within dendritic processes may be disrupted by the SCA5 mutant spectrins and compromise neuronal function. Because Purkinje cells are among the largest neurons in the human central nervous system (Palay and Chan-Palay, 1974), decreased efficiency in transport of macromolecules, vesicles, and organelles, as well as the compromised stability of specialized neuronal membrane domains may underlie the profound Purkinje cell loss in SCA5 patients.
Materials and methods
Fly stocks and genetic analyses
Flies were cultured at 25°C in a 12-h light/dark cycle on standard medium. Fly stocks were obtained from the Bloomington Drosophila Stock Center unless indicated and are listed in FlyBase (http://flybase.org/).
To analyze transgene expression in the eye, homozygous virgins for each genotype were crossed to gmr-GAL4/CyO males. Progeny lacking curly wings were examined. Neuronal expression was driven with elav-GAL4. Briefly, elav-GAL4/Y males were mated to homozygous virgins for each genotype. Female larvae were selected for further analysis.
To generate transgenic flies expressing syt-GFP in all neurons or n-syb-GFP in motor neurons, homozygous virgins carrying wild-type or mutant spectrin transgenes were crossed to either elav-GAL4–syt-GFP/Y (Zhang et al., 2002) or D42-GAL4–n-syb-GFP/TM6B, Tubby (Tb) males. GFP-positive larvae were selected for analyses.
To analyze genetic interaction of spectrin mutations with DHC64C6-10 (Gepner et al., 1996) and Gl1 (Harte and Kankel, 1982) mutants in the eye, standard meiotic recombination was used to generate second chromosomes carrying both the eye-specific gmr-GAL4 driver and either the hSPAM or the FSPGM transgenes. Recombinant chromosomes were maintained over a balancer chromosome carrying the dominant maker Curly (CyO). Virgin females from gmr-GAL4-hSPAM/CyO or gmr-GAL4-FSPGM/CyO lines were mated to Dhc64C6-10/TM6B, D or Gl1Sb/LVM males. Progeny carrying the recombinant chromosomes in either the Gl1 or DHC64C6-10 mutant backgrounds were identified by the absence of the dominant curly wing phenotype; the Gl1 mutation was identified by the bristle phenotype caused by the dominant Sb mutation, whereas adult flies carrying the DHC64C6-10 mutation lacked the curly and Dichaeted wing phenotypes.
To generate larvae expressing the American SCA5 mutation in all neurons in a dynein or dynactin mutant background, homozygous virgin females (hSPAM/hSPAM) were crossed to elav-GAL4-syn-GFP/Y; Dhc64C6-10/TM6B, Tb or elav-GAL4-syn-GFP/Y; Gl1Sb/TM6B, Tb males. Third instar larvae that carry GFP and lack the Tb marker were selected for analysis.
The RNAi transgenic stock used to deplete β-spectrin in Drosophila larvae was provided by G. Davis (University of California, San Francisco, San Francisco, CA).
Generation of the
UASp–β-Spec and UAST-β-Spec transgenic flies
To express human β-III–spectrin in Drosophila, full-length wild-type and mutant (American SCA5 mutation) 5′ myc-β-III–spectrin cDNA clones (Ikeda et al., 2006) were digested with KpnI and XbaI and ligated into the Drosophila transformation vector pUASp (Rørth, 1998). For generating the German SCA5 transgene, the L253P mutation was introduced into the wild-type 5′ myc-tagged β-III–spectrin construct (Ikeda et al., 2006) using the QuikChange II XL Site-Directed Mutagenesis kit (Agilent Technologies) and primers German mutant forward (5′-GGGACTTACCAAGCCGCTGGATCCCGAAGAC-3′) and German mutant reverse (5′-GTCTTCGGGATCCAGCGGCTTGGTAAGTCCC-3′). The resulting product was cloned into pUASp. Generated UASp constructs containing the human spectrin (hSP) sequences are referred to here as hSPWT (wild-type), hSPAM (American mutation), and hSPGM (German mutation; Fig. S1 B).
The construct designed to overexpress wild-type fly β-spectrin (FSPWT; Fig. S1 C) was created by generating separate PCR products. Briefly, a partial cDNA clone (AT24411; Drosophila Genomics Resource Center, Indiana University, http://dgrc.cgb.indiana.edu/) containing the 5′ region of the fly β-spectrin mRNA was used to amplify a KpnI–BamHI PCR fragment using primers fly forward KpnI (5′-GACGGTACCGCCAAGTGAAGTTCATCC-3′) and fly reverse BamHI (5′-CGTACTCATCGATGTACTCGTTGCC-3′). The remained 3′ cDNA sequence was cloned using the SuperScript III One-Step RT-PCR system with Platinum Taq high fidelity (Invitrogen), total RNA from fly embryos, and the gene-specific primers fly forward BamHI (5′-CAGCCATGACGACGGACATTTCG-3′) and fly reverse XbaI (5′-AGTCTAGAGCTCTTGCTTATGGTTGCG-3′). Both PCR products were then subcloned using subsequent KpnI–BamHI and BamHI–XbaI digestions into the corresponding cloning sites of pBluescript SK+ (Agilent Technologies) to generate the pBluescript SK+/β-Spec wild-type clone.
The American SCA5 mutant clone (FSPAM; Fig. S1 C) was generated by a PCR approach using primers fly American-Δ39 bp BglII forward (5′-CGTATATAAGATCTCGTTGGACAACATGGAGGAGATC-3′) and Fly American-Δ39 bp BsrGI reverse forward (5′-ACATCAGCTTGTACAGGCTGAGGGCGT-3′). The resulting PCR product, which deleted a 39-bp fragment in the region of homology with the deletion mutation found in the American SCA5 family (Fig. S1 B, I), was subcloned BglII–BsrGI into the pBluescript SK+/β-Spec wild-type clone. Both wild-type and the American SCA5 mutant full-length cDNAs were then cloned with KpnI–XbaI into the Drosophila transformation vector pUAST (Brand and Perrimon, 1993).
The German SCA5 mutant clone (FSPGM; Fig. S1 C) was generated by introducing the L253P mutation into the FSPWT clone using the QuikChange II XL Site-Directed Mutagenesis kit and primers fly German mutant forward (5′-CCTGGCCAAGCCCCTTGATGCCGAG-3′) and fly German mutant reverse (5′-GTCTTCGGGATCCAGCGGCTTGGTAAGTCCC-3′).
The integrity of each construct was verified by sequencing. Transgenic flies were generated by standard P element–mediated germline transformation. Multiple lines of flies with independent transgene insertion were established for each genotype.
Preparation of protein extracts
Adult flies for each genotype in the study were frozen in liquid nitrogen before heads were collected and homogenized in radio immunoprecipitation assay (RIPA) lysis buffer (1× PBS, 1% Nonidet P-40, 0.5% sodium deoxycholate, and 0.1% SDS) supplemented with Complete Protease Inhibitor Cocktail Tablets (Roche). The protein supernatant was collected after centrifugation at 14,000 rpm for 30 min at 4°C and used for immunoprecipitation and Western blot analyses.
Western blot analysis
Total proteins from fly heads were separated on 4–12% gradient NuPAGE gels (Invitrogen). After electrophoresis, gels were electroblotted overnight onto nitrocellulose membranes and incubated with β-III–spectrin (1:250; Santa Cruz Biotechnology, Inc.), tubulin (1:5,000, Sigma-Aldrich), or myc (1:2,000, clone 9E10; Sigma-Aldrich) antibodies. Rabbit polyclonal anti–Drosophila β-spectrin antibody (a gift from D. Branton, Harvard University, Cambridge, MA) was used at a 1:5,000 dilution. Immunoreactive bands were visualized using HRP-conjugated secondary antibodies at 1:5,000 dilutions and the ECL Western blotting detection system (GE Heathcare). The ImageJ analysis software (National Institutes of Health; http://rsb.info.nih.gov/ij/) was used to quantitatively compare protein expression.
Protein extracts from fly heads were precleared by the addition of 100 µl of a 1:1 protein A–Sepharose suspension (GE Healthcare). After incubation on a rotator at 4°C for 2 h, this mix was centrifuged for 5 min at 5,000 rpm. Supernatant aliquots containing 400 µg of total proteins were subsequently used in immunoprecipitation experiments. In parallel, 40 µl of protein A–Sepharose beads were equilibrated in RIPA buffer supplemented with protease inhibitors and further incubated with either 10 µg of mouse anti–Drosophila α-spectrin antibody (clone 3A9; Developmental Studies Hybridoma Bank, University of Iowa) or 50 mg/ml of BSA during 1 h at 4°C. The antibody–bead complexes were washed free of unbound antibody and incubated with precleared protein lysates overnight at 4°C. The protein A–Sepharose beads were then collected by centrifugation for 2 min at 2,500 rpm at 4°C and washed five times with ice-cold RIPA buffer. Bound proteins were eluted with 2× SDS sample buffer and subjected to SDS-PAGE and Western blot analysis.
Scanning electron and optical microscopy of fly eyes
For scanning EM images, fly heads were dehydrated in 100% ethanol, incubated in hexamethyldisilazane (Sigma-Aldrich), dried overnight under a vacuum, and coated with gold-palladium. Images were taken with a variable pressure scanning electron microscope (S-3500N; Hitachi). For optical images, adult flies eyes were photographed with a digital camera (Nikon) attached to a stereoscope (Carl Zeiss, Inc.).
For histological analysis of eyes, heads from 10-d-old flies were fixed for 30 min in a 2% glutaraldehyde/2% osmium tetroxide mix, washed in PBS, fixed for an additional 3 h in 2% osmium tetroxide, and dehydrated through a graded ethanol series. Heads were further equilibrated in propylene oxide and embedded in Durcupan ACM resin (Electron Microscopy Science). Vertical semithin sections were stained with toluidine blue and photographed with a microscope (AS/LMD; Leica).
The behaviors of larvae were compared under a stereoscope (Carl Zeiss, Inc.). Videos of animals expressing mutant and wild-type spectrin transgenes were made using a camcorder (Nikon).
For immunostaining of segmental nerves, wandering third instar larvae were dissected in 1× PBS and fixed in 4% formaldehyde for 20 min as described previously (Hurd and Saxton, 1996). Larval fillets were washed three times in PBT (1× PBS and 0.1% TritonX-100) and washed for 1 h in PBT containing 1% BSA (PBT-BSA) before overnight incubation with primary antibodies at 4°C. Primary antibodies used were mouse anti-CSP (6D6, 1:250; Developmental Studies Hybridoma Bank), mouse anti-dynein heavy chain antibody (P1H4, 1:250; McGrail and Hays, 1997), and mouse anti–α-tubulin (DM1A, 1:200; Sigma-Aldrich).
To examine synaptic morphology, third instar larvae were processed and stained as described in the previous paragraph, but the ventral ganglia, brain, and segmental nerves were removed to facilitate NMJ visualization. Muscle surfaces were delimited by Alexa Fluor 488 phalloidin (Invitrogen) staining. Larval preparations were incubated overnight with primary antibodies as follows: mouse anti-futsch (22C10, 1:100), mouse anti–Fas II (1D4, 1:10), mouse anti–discs large (4F3, 1:50), mouse anti-neuroglian (BP104, 1:10; all provided by the Developmental Studies Hybridoma Bank, University of Iowa), and rabbit anti–Ank2-XL (1:1,000; a gift from H. Aberle, Westfälische Wilhelms-Universität Münster, Münster, Germany). Signals were detected with Alexa Fluor 555, Alexa Fluor 488, and Cy5-conjugated secondary antibodies (Invitrogen) used at final concentrations of 1:200 at room temperature for 2 h. Larval preparations were washed in PBT-BSA and mounted in Vectashield (Vector Laboratories).
Images of neuromuscular preparation were acquired using an inverted microscope (Eclipse TE200; Nikon) equipped with a confocal imaging system (PerkinElmer) and a digital camera (Orca ER; Hamamatsu Photonics). Synapse morphology and axonal staining were imaged using 2 × 2 binning with a 40× Plan-Apochromat (NA 1.3) and a 100× Plan-Apochromat (NA 1.4) objective, respectively, and 1-µm optical sections. Images were processed in Photoshop CS3 and Illustrator CS4 (both from Adobe).
Live imaging of GFP-tagged vesicles in larval segmental axons
Motility analyses were done on neuromuscular preparations by live confocal fluorescence microscopy. Briefly, wandering third instar larvae were dissected in HL3 buffer (Feng et al., 2004; Stewart and McLean, 2004), carefully removing gut and fat body to expose segmental nerves. GFP vesicles were imaged at 25°C with an inverted microscope (Eclipse TE200; Nikon) equipped with the confocal imaging system (PerkinElmer). Images were acquired continuously at a rate of one frame every 1 s for 300 s, with 2 × 2 binning and a 100× oil Plan-Apochromat (NA 1.4) objective, using MetaMorph software (GE Healthcare) to control a charge-coupled device camera (Orca ER).
To analyze synaptogenesis, we determined the number of synaptic boutons present per muscle area (relative synapse size). In brief, the surfaces of muscles 6 and 7 of abdominal segments 2 and 3 were delimited after Alexa Fluor 488 phalloidin staining, and total muscle surface area was determined using the “measurement” function of the MetaMorph image analysis software (MDS Analytical Technologies). The relative synapse size was calculated as the total number of synaptic boutons divided by the corresponding total muscle surface area. This relative synaptic terminal size was then averaged for each genotype and normalized as a percentage of the control numbers.
Analysis of syt-GFP accumulations within larval segmental nerves was performed as follows. Four segmental nerves were selected from the region immediately posterior to the ventral ganglion, and GFP accumulations with a diameter of at least 0.7 µm were counted along a 50-µm axonal segment, using the “analyze particle” function of the MetaMorph software. In the analyses, each genotype is represented by five larval preparations.
The movement of GFP-tagged synaptic vesicles was manually tracked with MetaMorph’s “track points” function, as described previously (Mische et al., 2007). To define the direction of organelle movement, segmental nerves were oriented with the motor neurons cell bodies toward the left. Vesicle displacements toward the right were considered anterograde or plus-end runs, whereas right-to-left motions were designated retrograde or minus-end runs. Particles displaying linear movement for four consecutive frames were selected for analysis. Data from GFP particle tracking were used to calculate net velocity, mean run velocity, run length, and standard deviation for each particle using Excel software (Microsoft). Net velocity was determined when the directionality of motion could not be determined by summing all velocities displayed for one individual particle over the entire movie interval, including forward runs (positive), reverse runs (negative), and pauses. The trajectory of GFP-tagged synaptic vesicles along a portion of the segmental axon was graphed using the “kymograph” function of MetaMorph.
Statistical significance of the difference between mutant and wild-type was determined for each experiment using the Student’s t test on unpaired data. Significance was established when P < 0.05.
Online supplemental material
Fig. S1 shows generation of transgenic flies expressing the SCA5 mutations. Fig. S2 shows that expression of SCA5 mutant spectrin disrupts the morphology of NMJ synapses and the organization of the cell adhesion molecule Fas II. Fig. S3 shows that expression of SCA5 mutant spectrins does not result in synapse retraction or changes in ankyrin localization at NMJ synapses. Fig. S4 shows that expression of the SCA5 mutant transgenes does not severely disrupt the organization of the microtubule cytoskeleton. Fig. S5 shows analysis of human β-III and Drosophila β-spectrin protein expression in lines used for genetic interaction analysis. Video 1 shows that Drosophila third instar larvae overexpressing wild-type fly β-spectrin in all neurons driven by elav-GAL4 show a normal crawling behavior. Video 2 shows that Drosophila third instar larva expressing the American SCA5 mutation in fly β-spectrin develops a “tail flip” phenotype during crawling. Video 3 shows that Drosophila third instar larva expressing the German SCA5 mutation in fly β-spectrin develop a “tail flip” phenotype during crawling. Video 4 shows that Drosophila third instar larva expressing two copies of the hSPAM transgene and higher levels of the American mutant human β-III–spectrin develop a “tail flip” phenotype during crawling. Video 5 shows motion of syt-GFP bearing synaptic vesicles in Drosophila segmental nerves expressing wild-type fly β-spectrin driven by elav-GAL4 (elav-GAL4–syt-GFP/+; FSPWT/+). Video 6 shows the motion of syt-GFP–bearing synaptic vesicles in Drosophila segmental nerves expressing the German SCA5 mutant spectrin (elav-GAL4–syt-GFP/+; FSPGM/+). Video 7 shows the motion of GFP-n-syb–bearing synaptic vesicles in the motor axons of a Drosophila third instar larvae overexpressing wild-type fly β-spectrin (D42-GAL4–n-syb-GFP/+; FSPWT/+). Video 8 shows the motion of GFP-n-syb–bearing synaptic vesicles in the motor axons of a Drosophila third instar larvae expressing the German SCA5 mutant spectrin (D42-GAL4–n-syb-GFP/+; FSPGM/+).
We thank SCA5 family members for their participation, D. Branton for spectrin antibodies, H. Aberle for ankyrin antibodies, G. Davis for spectrin RNAi transgenic stocks, and H. Orr and the members of the Ranum and Hays laboratories for helpful discussions.
This work was supported by a National Institutes of Health (NIH) fellowship (1F31-NS056466) to D.N. Lorenzo; grants from the National Ataxia Foundation, the Bob Allison Ataxia Research Center, and the NIH (RO1NS056158) to L.P.W. Ranum; and an NIH award (RO1GM44757) to T.S. Hays.
cysteine string protein
excitatory amino acid transporter 4
- Fas II
fly spectrin American mutant
fly spectrin German mutant
fly spectrin wild type
human spectrin American mutation
human spectrin German mutation
human spectrin wild type
radio immunoprecipitation assay
spinocerebellar ataxia type 5