Analysis of transport parameters for anterograde and retrograde syb-GFP containing vesicles in wild-type and mutant segmental nerves
| Genotype | Anterograde runs | Retrograde runs | ||||||||
| Velocity | P-value | Run length | P-value | n | Velocity | P-value | Run length | P-value | n | |
| µm ± SD | µm/s ± SD | µm/s ± SD | µm ± SD | |||||||
| D42-GAL4; FSPWT | 0.78 ± 0.13 | NA | 9.6 ± 2.5 | NA | 54 | −0.72 ± 0.15 | NA | 9.2 ± 3.1 | NA | 80 |
| D42-GAL4/FSPAM | 0.5 ± 0.17* | <0.0001 | 6.8 ± 3.1* | <0.0001 | 76 | −0.55 ± 0.11* | <0.0001 | 7.2 ± 2.5* | <0.0001 | 97 |
| D42-GAL4; FSPGM | 0.44 ± 0.15* | <0.0001 | 6.1 ± 2.7* | <0.0001 | 81 | −0.48 ± 0.18* | <0.0001 | 7.4 ± 3.3* | <0.0001 | 135 |
| Genotype | Anterograde runs | Retrograde runs | ||||||||
| Velocity | P-value | Run length | P-value | Velocity | P-value | Run length | P-value | |||
| 0.78 ± 0.13 | NA | 9.6 ± 2.5 | NA | 54 | −0.72 ± 0.15 | NA | 9.2 ± 3.1 | NA | 80 | |
| 0.5 ± 0.17* | <0.0001 | 6.8 ± 3.1* | <0.0001 | 76 | −0.55 ± 0.11* | <0.0001 | 7.2 ± 2.5* | <0.0001 | 97 | |
| 0.44 ± 0.15* | <0.0001 | 6.1 ± 2.7* | <0.0001 | 81 | −0.48 ± 0.18* | <0.0001 | 7.4 ± 3.3* | <0.0001 | 135 | |
Mean velocity and run length for both anterograde and retrograde moving particles are reduced in mutant β-spectrin larvae in comparison to wild type. Data shown represent mean ± SD. Asterisks denote statistical significant differences for mutant relative to wild type. NA, not applicable.
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