The Saccharomyces cerevisiae DJP1 gene encodes a cytosolic protein homologous to Escherichia coli DnaJ. DnaJ homologues act in conjunction with molecular chaperones of the Hsp70 protein family in a variety of cellular processes. Cells with a DJP1 gene deletion are viable and exhibit a novel phenotype among cytosolic J-protein mutants in that they have a specific impairment of only one organelle, the peroxisome. The phenotype was also unique among peroxisome assembly mutants: peroxisomal matrix proteins were mislocalized to the cytoplasm to a varying extent, and peroxisomal structures failed to grow to full size and exhibited a broad range of buoyant densities. Import of marker proteins for the endoplasmic reticulum, nucleus, and mitochondria was normal. Furthermore, the metabolic adaptation to a change in carbon source, a complex multistep process, was unaffected in a DJP1 gene deletion mutant. We conclude that Djp1p is specifically required for peroxisomal protein import.
Peroxisomes are organelles present in most eukaryotic cells. Proteins destined for the peroxisomal matrix are synthesized on free polyribosomes and are imported posttranslationally (Lazarow and Fujiki, 1985). Two types of peroxisomal targeting signals (PTSs)1 have been identified. The PTS1 consists of a COOH-terminal tripeptide consisting of the amino acids serine, lysine, and leucine (SKL) or a derivative thereof (Gould et al., 1988; Purdue and Lazarow, 1994; Elgersma et al., 1996b). The PTS2 is an NH2-terminal sequence found in a limited number of peroxisomal matrix proteins (Osumi et al., 1991; Swinkels et al., 1991; Purdue and Lazarow, 1994). In addition, some internal targeting sequences have been reported for matrix proteins (Small et al., 1988; Kragler et al., 1993; Elgersma et al., 1995) and for integral membrane proteins (McCammon et al., 1994; Dyer et al., 1996; Elgersma et al., 1997).
Specific cytosolic receptors have been identified for PTS1- and PTS2-containing proteins. These receptors have been proposed to function as cycling receptors (Dodt and Gould, 1996; Elgersma et al., 1996a, 1998; Gould et al., 1996; Rehling et al., 1996), which (a) bind their ligand in the cytoplasm; (b) dock at the peroxisomal membrane; and (c) dissociate from the ligand and return to the cytoplasm. It is unclear whether these receptors dissociate from their ligand at the cytoplasmic surface of the peroxisomal membrane or whether the complex enters the peroxisomal matrix before dissociation.
The actual translocation of polypeptides across the peroxisomal membrane seems to differ from translocation of (partially) unfolded precursor proteins across the ER and mitochondrial membranes. Some peroxisomal proteins have been shown to oligomerize in the cytoplasm before import (Glover et al., 1994; McNew and Goodman, 1994; Elgersma et al., 1996b; Leiper et al., 1996). Import studies in semi-intact cells and microinjection experiments have revealed that complete protein unfolding is not required for translocation (Walton et al., 1992; Wendland and Subramani, 1993; Walton et al., 1995). In this respect, the peroxisomal protein import process may resemble the nuclear protein import process, where folded and assembled proteins can enter the organelle (Melchior and Gerace, 1995).
Microinjection of an artificial substrate protein (biotinylated human serum albumin coated with PTS1-containing peptides) into fibroblasts revealed the requirement for a cytosolic Hsp70 for association of this protein with peroxisomes (Walton et al., 1994). The Hsp70 chaperone system is involved in a variety of processes including translation, protein degradation, folding and assembly of newly synthesized proteins, and their transport to particular subcellular compartments (Hartl, 1996; Hayes and Dice, 1996). Hsp70s can also regulate the composition of protein complexes (Frydman and Höhfeld, 1997). In eukaryotic cells, different classes of Hsp70 proteins are present that are not functionally interchangeable (Craig et al., 1994).
Hsp70s function in conjunction with partner proteins, among which are the J-domain–containing proteins. This protein family consists of modular proteins that contain an evolutionarily conserved J-domain (first identified in Escherichia coli DnaJ). The J-domain specifies the interaction with a particular Hsp70 (Schlenstedt et al., 1995). Other domains interact with the substrates for the Hsp70 machinery. By combining the J-domain with other protein modules, a J-domain–containing protein can recruit a particular Hsp70 to a site in the cell where it can perform one of its specific tasks (Rassow et al., 1995). Two cytosolic J-proteins have been well characterized in Saccharomyces cerevisiae. Sis1p is an essential protein that is required for initiation of translation (Zhong and Arndt, 1993). Ydj1p, on the other hand, is required for import of ER and mitochondrial proteins in vivo (Atencio and Yaffe, 1992; Becker et al., 1996), for Cdc28p-dependent phosphorylation and degradation of Cln3p and for ubiquitin-dependent degradation of short-lived and abnormal proteins (Lee et al., 1996; Yaglom et al., 1996). Δydj1 cells grow very slowly and display pleiotropic morphological defects (Caplan and Douglas, 1991).
We have identified the DJP1 gene by functional complementation of a previously described peroxisome assembly mutant pas22-1 (Elgersma et al., 1993). The DJP1 gene encodes a cytosolic J-domain–containing protein. In contrast to the cytosolic J-domain proteins Sis1p and Ydj1p, Djp1p function is restricted to peroxisomes: Djp1p was dispensible for growth on a wide variety of fermentable and non-fermentable carbon sources, except when peroxisome functioning was required. In Δdjp1 cells, peroxisomal matrix proteins were partially mislocalized to the cytosol and peroxisomes failed to grow to full size. Besides atypical peroxisomal structures, no additional morphological abnormalities were observed. Furthermore, import of proteins into the ER, mitochondria, and nucleus was unaffected in Δdjp1 cells. Our results provide genetic evidence for the involvement of cytosolic chaperones in peroxisomal protein import.
Materials And Methods
Yeast Strains and Culture Conditions
The yeast strains used in this study were S. cerevisiae BJ1991 (Matα, leu2, trp1, ura3-251, prb1-1122, pep4-3, gal2) (Jones, 1977) and BSL1-2C (Leighton and Schatz, 1995). The pex6 deletion mutant was generated in our laboratory (Voorn-Brouwer et al., 1993). The djp1 deletion mutant is described below. Pas22-1 was isolated by Elgersma et al. (1993). Yeast transformants were selected and grown on minimal medium containing 0.67% yeast nitrogen base without amino acids (YNB-WO; DIFCO Laboratories, Detroit, MI), 2% glucose and amino acids (20–30 μg/ml) as needed. The liquid media used for growing cells for nucleic acid isolation, subcellular fractionation, immunogold electron microscopy, and enzymatic assays contained 0.5% potassium phosphate buffer, pH 6.0, 0.3% yeast extract, 0.5% peptone, and either 2% glucose, 3% glycerol or 0.1% oleate/2% Tween 40. Before shifting to these media, the cells were grown on 0.3% glucose medium for at least 24 h. Oleate plates contained 0.67% yeast nitrogen base without amino acids (YNB-WO; DIFCO Laboratories), 0.1% yeast extract (DIFCO Laboratories), 2% agar, 0.1% oleate (vol/vol), 0.25% Tween 40 (vol/vol), and amino acids as needed. For the galactose-induction experiments, cells were grown on YPD and shifted to YPgal (1% yeast extract, 2% bacto peptone, and either 2% glucose or 2% galactose).
The impaired growth of pas22-1 cells on oleate plates was used for cloning of the DJP1 gene by functional complementation with a S. cerevisiae genomic library constructed in Ycp50. Library transformants were selected on low minimal glucose plates and subsequently replica plated onto oleate plates. Complementing plasmids were rescued in E. coli and retransformed to pas22-1 cells to confirm plasmid-linked complementation. One plasmid was selected for further characterization (p22.1). p22.1 was partially digested with Sau3a, size-fractionated into pools of 0.5–1 kb, 1–2 kb, 2–3 kb, 3–4 kb, etc., and cloned into the BamHI site of Ycplac33KanR (van der Leij et al., 1992). From the different library pools, the 1–2-kb pool was still able to complement the mutant. The smallest complementing plasmid from this pool that rescued the mutant contained an insert of 1,200 bp (p22.2). Both strands were sequenced by the dideoxy chain-termination method. The obtained nucleotide and predicted amino acid sequence was compared with the Saccharomyces cerevisiae Genome Database. p22.2 encoded the COOH-terminal part of YIR004w, starting at nucleotide 184.
The DJP1 gene deletion construct was prepared by insertion of the LEU2 gene between the AseI site 212 bp before the start of the DJP1 open reading frame (ORF) and the internal BglII site. This plasmid was linearized and transformed to BJ1991 and BSL1-2C cells. Deletion mutants were selected on leucine-deficient plates and checked by Southern blot analysis for proper integration.
Green fluorescent protein (GFP)-PTS1 and PTS2-GFP expression plasmids were constructed by cloning either a BamHI–XbaI fragment or a filled-in HindIII–XbaI fragment from pSM102 and pJM205 (Kalish et al., 1996) into pEL44, respectively, thereby creating pEW88 and pFM1. pEL44 is derived from a centromere-containing URA3 plasmid (Gietz and Sugino, 1988) containing the oleate-inducible catalase A promoter and 3′ UTR (Elgersma et al., 1993).
Plasmids encoding preproα-factor and Suc2 (invertase) fusions with GFP-HDEL were prepared as follows. An EagI restriction site upstream of the GFP translation initiation codon and the last nine codons of Kar2p, including the ER retention signal HDEL, were added to GFP ORF by PCR with the following oligonucleotides: EAG1 primer, TTTTCGGCCGAAAAGATGAGTAAAGGAGAAGAACTT; HDEL primer, TTTAAGCTTACAATTCGTCGTGTTCGAAATAATCACCTTTGTATAGTTCATCCATGC. The PCR product was digested with HindIII and EagI and cloned into PSY1 and PSY16 (Harmsen et al., 1993) yielding the plasmids pEW109 and pEW110. Subsequently, SacI–HindIII fragments from pEW109 and pEW110 were cloned into pEW70, a pEL44 derivative containing a TRP1-selectable marker (Hettema et al., 1996) yielding the plasmids pEW111 and pEW112 for oleate-dependent expression of preSuc2-GFP-HDEL and preproα-GFP-HDEL. NLS-GFP was constructed by cloning GFP into pGAD (Shulga et al., 1996). The plasmid encoding NH2-tagged Mdh3p was described before (Elgersma et al., 1996a).
Production of Djp1p Polyclonal Antiserum
Part of Djp1p was expressed in E. coli by cloning a PstI fragment of the DJP1 gene (encoding the COOH-terminal part starting at amino acid residue 137) in plasmid pQE-10 (QIAGEN Inc., Chatsworth, CA). The six histidine residues fused to the Djp1p fragment allowed rapid purification by nickel-chelating chromatography under denaturing conditions according to the manufacturer's instructions. The protein was further purified by SDS-PAGE, visualized with 0.25 M KCl/1 mM DTT, and subsequently excised and eluted in 50 mM Tris/HCl (pH 8.0)/0.1% SDS/0.1 mM EDTA/ 5 mM DTT/0.15 M NaCl, and then was used to immunize rabbits.
A four-step floatation gradient was made. An organellar 17,000 g pellet was resuspended in 400 μl 50% Nycodenz in gradient buffer (5 mM MES (pH 5.5)/1 mM KCl/1 mM EDTA/1 mM PMSF) and transferred to a Beckman tube. This pellet fraction was overlaid with 1.6 ml of 40% Nycodenz, 1.6 ml 30% Nycodenz, and 1.6 ml of 0% Nycodenz in gradient buffer containing 0.65 M sorbitol, and then centrifuged for 18 h at 150,000 g at 4°C, and fractions were analyzed by Western blotting. In the same experiment, an organellar pellet was resuspended in 0.65 M sorbitol in gradient buffer and applied on top of the step gradient and centrifuged in parallel.
Nycodenz gradients presented in Fig. 6 are continuous 16–35% Nycodenz equilibrium density gradient (12 ml), with a cushion of 1 ml 42% Nycodenz dissolved in gradient buffer containing 8.5% sucrose. The sealed tubes were centrifuged during 2.5 h in a vertical rotor (MSE 8×35) at 19,000 rpm (29,000 g) at 4°C. Fractions were analyzed for marker proteins with activity measurements or Western blotting.
Protease Protection, SDS-PAGE and Western Blotting
Cells grown overnight on oleate medium were converted to spheroplasts with Zymolyase 100T (1 mg/g cells). The spheroplasts were washed twice in 1.2 M sorbitol in gradient buffer. The washed spheroplasts were lysed by osmotic shock in 0.65 M sorbitol in gradient buffer. Intact cells and nuclei were removed from the homogenate by two centrifugation steps at 600 g for 10 min. For protease protection experiments, 100 μl of homogenate with a protein concentration of 1 mg/ml was added to 100 μl 0.65 M sorbitol in gradient buffer containing proteinase K (100 μg) either in the presence or absence of 0.3% Triton X-100. The samples were incubated at room temperature for 0, 15, or 30 min. The reaction was stopped by the addition of TCA (10%). Samples were analyzed by Western blotting. Western blots were incubated with rabbit polyclonal antisera raised against the various proteins. Antibody complexes were either detected by incubation with goat anti–rabbit Ig-conjugated alkaline phosphatase or peroxidase.
To study protein import into the ER, exponentially growing cells were harvested and resuspended into 600 μl of fresh 2% minimal glucose medium at OD600 = 20. Cells were preincubated at 37°C for 30 min to inactivate Sec62p in Sec62ts cells. Subsequently, 100 μCi of [35S]methionine/ [35S]cysteine was added and cells were incubated at 28°C for 5 min. The chase was started by the addition of 6 mM each of unlabeled methionine and cysteine to the reaction mixture followed by further incubation at 28°C for 0, 5, or 30 min. Chase reactions were stopped on ice. To prepare glass bead lysates, cells were harvested and resuspended in 250 μl PBS/ 0.5% Triton X-100/20 mM NEM/1 mM PMSF. After addition of 100 μl glass beads, cells were vortexed for 30 min at maximal speed at 4°C. Glass beads and cell debris were removed by centrifugation at 17,000 g for 2 min at 4°C. 100 μl of supernatant was used for immunoprecipitation with 10 μl anti-Kar2p rabbit polyclonal antiserum and 50 μl protein A–Sepharose. Incubations were performed overnight at 4°C. Immunoprecipitates were washed twice with 10 mM Tris/HCl, pH 8.6, containing 300 mM NaCl, 0.05% Triton X-100, and 0.1% SDS, and were analyzed by SDS-PAGE and fluorography.
For protein import studies into mitochondria the same conditions were used as described above, except that cells were not preincubated at 37°C. In addition, 0.02% azide was added after each chase time point before cells were put on ice, 5 μl anti-Hsp60 rabbit polyclonal antiserum was used, and immunoprecipitates were washed twice with 100 mM Tris/HCl, pH 8.6, containing 300 mM NaCl, 0.05% Triton X-100, and 0.05% SDS.
Galactose Induction Experiment
For these experiments, we used the S. cerevisiae strain BSL1-2c and BSL1 djp1::leu2, because our wild-type strain BJ1991 is GAL2− (Jones, 1977). Cells were grown in YPD medium (late log phase) before inoculation (OD600 = 0.25–0.3) in YPgal. After each time point, the OD600 was measured and cells were washed and frozen at −20°C before glass bead lysates (in PBS/0.5% Triton X-100/1 mM PMSF) were prepared. Galactose-1-phosphate uridylyltransferase activity was measured by a spectrophotometric assay (Pesce et al., 1977).
3-Hydroxyacyl-CoA dehydrogenase activity was measured on a Cobas-Fara centrifugal analyzer by following the 3-keto-octanoyl-CoA–dependent rate of NADH consumption at 340 nm (Wanders et al., 1990). Succinate dehydrogenase activity was measured according to Munujos et al. (1993). Protein concentrations were determined by the bicinchoninic acid method (Smith et al., 1985). Subcellular fractionations were performed as described (Van der Leij et al., 1992). Organellar pellets (17,000 g pellets) were used for continuous Nycodenz gradients as described (van Roermund et al., 1995). DNA manipulations were performed as described (Sambrook et al., 1989). Immunogold electron microscopy was done as described (Distel et al., 1988). Nuclear import assays were performed as described by Shulga et al. (1996).
Cloning and Expression of the DJP1 Gene
We have identified the DJP1 gene by functional complementation of the peroxisome assembly mutant pas22-1 (see Materials and Methods). DJP1 is identical to YIR004w, a 432–amino acid ORF on chromosome IX, identified during the course of the S. cerevisiae genome sequencing project (Voss et al., 1995). We deleted the YIR004w gene and allowed the disruption mutant to mate with pas22-1 cells. Both the diploid cells and the haploid gene deletion mutant showed the same growth characteristics as pas22-1 cells. Growth on oleic acid–containing medium was retarded, whereas growth on all other carbon sources (glucose, galactose, glycerol, acetate, and ethanol) was normal (not shown). In pas22-1 cells, we found a single base pair deletion (G1129) in the DJP1 gene. This alteration caused a frame shift at codon 376 resulting in premature termination of the ORF. These results identified YIR004w as the DJP1 gene. A polyclonal antiserum raised against the COOH-terminal 294–amino acid residues of Djp1p recognized Djp1p specifically on a Western blot (Fig. 1, lane 1), and this protein band was absent from Δdjp1 cell lysates (Fig. 1, lane 2). As expected, pas22-1 cells expressed a low molecular weight version of the DJP1 gene (Fig. 1, lane 3).
Since growth of Δdjp1 cells was retarded only on oleate media, we anticipated a role for Djp1p in peroxisome biogenesis. Peroxisome number and peroxisomal protein levels are regulated in response to fatty acids (oleate) in the media. The expression level of Djp1p, however, was constitutive; it was unaffected by culture conditions or heat shock (Fig. 1, lanes 4–7).
Djp1p contains an NH2-terminal J-domain (Fig. 2, A and B). This 70–amino acid domain is conserved throughout evolution and is named after E. coli DnaJ. EcDnaJ consists of several additional evolutionarily conserved domains, which Djp1p does not have (Fig. 2 A). Instead, Djp1p contains two predicted domains of unknown function.
Localization of Djp1p in the Cytosol
Using subcellular fractionation experiments, we studied the subcellular localization of Djp1p in cells grown overnight on oleic acid medium (Fig. 3,a). A homogenate (H) was prepared from spheroplasts by gentle osmotic lysis and fractionated by successive differential centrifugation into a 2,500 g pellet (P1), a 17,000 g pellet (P2), a 100,000 g pellet (P3) and a supernatant fraction (S) (Fig. 3 a). All four fractions were analyzed for the presence of organellar marker proteins, either by Western blot analyses or by enzymatic activity measurements. F1β-ATPase, Kar2p, and phosphoglucose isomerase (PGI) were used as markers for mitochondria, ER, and cytosol, respectively. We used peroxisomal catalase (catalase A), peroxisomal malate dehydrogenase (Mdh3p) and 3-ketoacyl-CoA thiolase (thiolase) as marker proteins for the peroxisomal matrix and the peroxisomal ABC transporter protein 1 (Pat1p) as marker for the peroxisomal membrane.
The fractionation profile of Djp1p was different from that of mitochondrial, peroxisomal and ER proteins. The bulk of Djp1p cofractionated with the cytosolic marker PGI in the 100,000 g supernatant (S) (Fig. 3,a) or even in a 200,000 g supernatant (not shown), indicating that Djp1p is a cytosolic protein. However, a fraction of Djp1p was pelleted at 17,000 g (P2). P2 contained a very low amount of PGI activity suggesting a marginal cytosolic contamination of P2, but this was not sufficient to explain the presence of Djp1p in P2. Djp1p was not necessarily associated with functional peroxisomes as Djp1p was also found in the 17,000 g pellet from cells that lack morphologically distinguishable peroxisomes, the Δpex6 cells (Fig. 3 a) and Δpex13 cells (not shown). The fractionation behavior of Djp1p was identical in glucose-grown cells.
We next aimed to identify the nature of the pelletable Djp1p fraction. The 17,000 g pellet derived from wild-type cells was therefore subjected to Nycodenz equilibrium density gradient centrifugation followed by Western blot analysis. In this gradient, Djp1p did not colocalize with peroxisomes and mitochondria but remained in the low density fractions (Fig. 3,b). The pelletable fraction of Djp1p apparently associated with a large structure, either as part of a protein complex or associated with membranes. To distinguish between these two possibilities, we prepared a 17,000 g organellar pellet from wild-type cells, resuspended it in 50% Nycodenz (wt/vol) and layered the suspension at the bottom of a Nycodenz floatation step gradient (see Materials and Methods). Membranes were floated to light density by overnight centrifugation at 150,000 g as monitored by the distribution of the organellar marker proteins (Fig. 3,c). The majority of Djp1p molecules migrated up into the gradient (Fig. 3,c, peak in fraction 8) implying its association with membranes. The Djp1p equilibration profile was distinct from that of peroxisomes, which peaked in fraction 5 (Fig. 3 c). Mitochondria and microsomes migrated with the bulk of the membranes to fractions 5–8, with a peak in fractions 7 and 8. We concluded that Djp1p was associated with membranes that overlap in density with, but are distinct from, the bulk of peroxisomes, mitochondria, and microsomes.
Since proteins may dissociate from membranes or from protein assemblies during homogenization of cells and subsequent subcellular fractionation, we wanted to confirm our results on the subcellular localization of Djp1p using a milder method. Spheroplasts prepared from oleate-grown cells were incubated with increasing concentrations of digitonin and the release of marker proteins was measured. At low digitonin concentrations, the plasma membrane was permeabilized selectively as monitored by the release of the cytosolic marker protein PGI (Fig. 3,d) with simultaneous retention of organellar markers. At higher digitonin concentrations intracellular membranes were permeabilized, upon which catalase and NH2-Mdh3p were released from the cells. Djp1p started to be released from cells at low digitonin concentrations, initially coeluting with PGI. However, by increasing the digitonin concentration gradually, more Djp1p was released. This illustrates that most of Djp1p behaved as a soluble cytosolic protein, whereas the rest was retained until digitonin concentrations were reached that solubilized intracellular membranes completely. The use of different carbon sources for growth did not affect the retarded release of Djp1p from digitonin-permeabilized cells (not shown). Furthermore, the more digitonin-resistant pool of Djp1p was not necessarily associated with functional peroxisomes, since the retarded release was also observed in digitonin-permeabilized Δpex6 cells (Fig. 3 d).
To firmly establish the cytosolic localization of Djp1p, and to determine whether the membrane-associated pool of Djp1p was located on the cytosolic side of the membranes, we used a third approach. We tested latency of the protein in a cell homogenate using protease digestion (Fig. 3,e). We therefore prepared detergent-free homogenates from wild-type cells, and treated them with proteinase K to degrade proteins that are not protected by a membrane. The control peroxisomal matrix protein thiolase was completely protected from digestion by the protease, whereas upon addition of the detergent Triton X-100 to open up the peroxisomal membranes, thiolase was cleaved (Fig. 3 e). Thiolase contains a single protease-sensitive site that is rapidly cleaved upon exposure to protease, whereas the remaining proteolytic product is protease resistant (Höhfeld et al., 1991). In contrast to thiolase, Djp1p was degraded rapidly and quantitatively by Proteinase K both in the absence and presence of Triton X-100. These experiments confirm and extend our differential centrifugation experiments and digitonin titration experiments that indicate that Djp1p was not incorporated into organelles. It appears mainly as a cytosolic protein, with a fraction associated with the cytosolic side of membranes.
Mislocalization of PTS-containing GFP in Δdjp1 Cells
The growth deficiency of Δdjp1 cells was restricted to media containing fatty acids as sole carbon source, which is suggestive of a defect in peroxisome functioning. To investigate whether peroxisome biogenesis was indeed affected in Δdjp1 cells, we determined whether proteins with a peroxisomal targeting signal can be imported into peroxisomes in these cells. We used both a PTS1- and a PTS2-containing variant of the GFP from the jellyfish Aequorea victoria (Kalish et al., 1995). The cDNAs encoding these proteins were cloned into a yeast expression vector containing the oleate-inducible regulatory sequence of the catalase A (CTA) gene (Elgersma et al., 1993). Cells transformed with the GFP-PTS1 or PTS2-GFP expression plasmids were grown to late logarithmic phase on selective glucose medium, and then transferred to oleate medium and analyzed 24 h later (Fig. 4).
In wild-type cells transformed with either construct, a few large clusters of fluorescent spots were present, indicating that both proteins efficiently entered peroxisomes (Fig. 4). In Δdjp1 cells, however, a punctate fluorescence pattern reminiscent of peroxisomes was observed against a background of pronounced diffuse labeling for both proteins. Prolonged culturing of wild-type cells on oleate media resulted in the formation of very large fluorescent structures, which were absent from Δdjp1 cells for both GFP-PTS1 (not shown) and PTS2-GFP (Fig. 4). Instead, the diffuse labeling of the cells increased and the peroxisomes remained small. We concluded that in Δdjp1 cells, only a fraction of PTS-containing GFP is associated with peroxisomes whereas the rest is mislocalized to the cytosol. After prolonged culture on oleate medium, atypically small peroxisomal structures are present in Δdjp1 cells.
Mislocalization of Peroxisomal Matrix Proteins in Δdjp1 Cells
To extend and confirm the results with GFP for endogenous peroxisomal proteins, and to determine whether the effects of DJP1 deletion are specific for the peroxisome, we examined the subcellular distribution of marker proteins for cytosol (PGI), mitochondria (F1β-ATPase), ER (Kar2p), and peroxisomes (catalase A, NH-Mdh3p as a typical PTS1 marker protein, thiolase as PTS2 marker protein, and Pat1p and Pex13p as markers for the peroxisomal membrane). Cells were grown for 16 h on oleate medium to allow optimal peroxisome induction and then fractionated as shown in Fig. 5. Whereas marker proteins for ER and mitochondria fractionated in Δdjp1 cells as in wild-type cells, the peroxisomal marker protein catalase A behaved clearly differently (Fig. 5,a). In contrast, NH-Mdh3p, thiolase, and the membrane proteins Pat1p (Fig. 5 a) and Pex13p were located mainly in the 17,000 g pellet (P2) of Δdjp1 cells. Hence, after 16 h growth on oleate, the most pronounced defect in Δdjp1 cells was the mislocalization of catalase A to the cytosolic fraction.
Since we had observed that the cytosolic labeling of GFP-PTS1 and PTS2-GFP increased with time after the shift to oleate medium, we wanted to find out whether the mislocalization of endogenous peroxisomal matrix proteins in Δdjp1 cells was dependent on cellular growth conditions as well. We grew Δdjp1 cells on oleate for 40 h and compared the distribution of peroxisomal marker proteins between the 17,000 g organellar pellet and the supernatant fraction (Fig. 5 b). Under these conditions, catalase A was recovered almost completely in a 17,000 g supernatant fraction. Moreover, thiolase was now also present in the supernatant fraction whereas NH-Mdh3p and the peroxisomal membrane proteins remained completely particulate.
The same experiment was performed with cells grown overnight on glycerol-containing medium (Fig. 5 c). These cells showed the most defective phenotype, with extensive mislocalization of NH2-Mdh3p as well as catalase A and thiolase. Our results indicate that the extent to which peroxisomal proteins cofractionated with the cytosolic marker protein varied with growth conditions in Δdjp1 cells. Optimal oleate induction partially restored the Δdjp1 phenotype. The level of Djp1p itself was not regulated in response to oleate in the medium and, inversely, the oleate response was not impaired in Δdjp1 cells as determined by the level of activity of peroxisomal β–oxidation enzymes (not shown). These results suggest that either Djp1p was functionally replaced by an oleate-inducible factor or the function of Djp1p was needed less under conditions of optimal oleate induction. Interestingly, serial analysis of gene expression (SAGE) of wild-type cells grown on oleate for 16 h revealed the induction of several heat-shock proteins that may render Djp1p partially redundant (Kal, A.J., and H.F. Tabak, personal communication).
Under both growth conditions, oleate as well as glycerol, marker proteins for ER, mitochondria and cytosol behaved as in wild-type cells (Fig. 5 a; and results not shown), indicating that the effects of DJP1 gene deletion were limited to peroxisomes (see below).
Low-Density Peroxisomal Structures in Δdjp1 Cells
To further characterize the peroxisomes in Δdjp1 cells, especially considering the punctate pattern shown in Fig. 4, we determined their density in a Nycodenz gradient. Fractionation of a 17,000 g pellet (P2) of either wild-type or Δdjp1 cells by Nycodenz equilibrium density gradient centrifugation revealed that the peroxisomal matrix protein thiolase comigrated with the peroxisomal membrane proteins Pex13p (Fig. 6) and Pat1p (not shown) in Δdjp1 cells. In contrast to peroxisomes in wild-type cells, which equilibrated at high density, the peroxisomes in Δdjp1 cells equilibrated at a broad density range, the bulk of them having a lower density than the peroxisomes in wild-type cells (Fig. 6).
Residual Protein Import into Small Spherical Peroxisomes in Δdjp1 Cells
The biochemical experiments revealed a partial association of matrix proteins with peroxisomes in Δdjp1 cells, and direct immunofluorescence suggested a partial peroxisomal localization for the GFP-PTS proteins. To determine whether these matrix proteins were indeed imported into the organelle, or whether they were just associated with the peroxisomal surface, we performed immunogold electron microscopy studies. The localization of CTA and NH2-Mdh3p in Δdjp1 cells was studied (Fig. 7,a). Antisera directed against NH2-Mdh3p (Fig. 7 a, large gold particles) and catalase A (small gold particles) specifically labeled the peroxisomal matrix in Δdjp1 cells, showing that matrix proteins were indeed imported into the peroxisomes of Δdjp1 cells.
In cross-sections of Δdjp1 cells grown on oleate for 40 h, peroxisomal structures were scarce and relatively small. As our subcellular fractionation studies predicted, the ratio of small gold particles (representing CTA) to large gold particles (NH2-Mdh3p) was much higher in wild-type cells than in Δdjp1 cells (Fig. 7, a and b). Hence, the amount of CTA relative to the amount of NH2-Mdh3p was low in peroxisomes of Δdjp1 cells when compared with wild-type cells.
Specificity of Djp1p for Peroxisomal Protein Import
Peroxisomes are indispensable for growth on oleate media but are not necessary for growth on other carbon sources such as glucose, glycerol, acetate, and ethanol. Peroxisomal protein import mutants therefore have been identified based on these growth characteristics. Disturbances of protein import into the endoplasmic reticulum (Novick et al., 1980) or nucleus (Doye and Hurt, 1995) are either lethal or have been shown to affect the growth rate on glucose media. A complete block in mitochondrial protein import is lethal, whereas mutation of import-stimulating factors has been shown to affect growth on non-fermentable carbon sources (Baker and Schatz, 1991). Thus, based on the growth characteristics of Δdjp1 cells, it is not likely that Djp1p is required for import of proteins into nuclei, ER, or mitochondria.
Our fractionation data shown in Fig. 5,a for the resident ER protein Kar2p and mitochondrial F1-ATPase confirm the specificity of Djp1p function for peroxisomes. To further exclude a relation between Djp1p and the ER, we expressed GFP fusion proteins containing an ER retention signal at the COOH terminus and either the presequence of invertase (Suc2p) or that of preproα-factor at the NH2 terminus (see Materials and Methods). Expression of the fusion proteins in wild-type cells resulted in a fluorescent pattern similar to that observed for Kar2p by indirect immunofluorescence microscopy (not shown). After growth on oleate for 24 h (Fig. 8 a) or 40 h (not shown), the GFP fusion proteins displayed the same distribution in Δdjp1 cells as in wild-type cells. This experiment indicated that ER morphology was normal and that import into the ER was not disturbed to such an extent that the steady state distribution of the GFP fusion proteins was affected in Δdjp1 cells.
If proteins are long-lived, a decrease in import rate is not necessarily reflected by a change in their steady state distribution. To address this, we studied the import rate of Kar2p in Δdjp1 cells by monitoring the processing of the precursor to the mature form (Fig. 8 b). Therefore, wild-type and Δdjp1cells were incubated with [35S]methionine and [35S]cysteine for 5 min (pulse), and the label was chased with cold methionine and cysteine during 5 and 30 min. We did not detect any accumulation of preKar2p in wild-type or Δdjp1 cells after 5 min of pulse-labeling, indicating rapid import and processing. As a control we used sec62ts cells, which clearly accumulated preKar2p. We concluded that preKar2p was rapidly imported into Δdjp1 cells. We again showed that Djp1p was not involved in the import of proteins into the ER.
To further exclude a role for Djp1p in protein import into mitochondria we analyzed the import of mitochondrial Hsp60 by following its processing from the precursor form. As a control, we used Δtom20 cells, which accumulate some mitochondrial precursor proteins in the cytosol, including Hsp60 (Ramage et al., 1993; Moczko et al., 1994). Western blot analysis of total lysates showed that in contrast to Δtom20 cells (Fig. 9, lane 3), Δdjp1 cells (Fig. 9, lane 2) did not accumulate precursor Hsp60, implying that protein import into mitochondria was not affected to such an extent that it led to accumulation of precursor Hsp60 in the cytosol. If import of Hsp60 was only slightly retarded, or if mitochondrial Hsp60 is long-lived, accumulation of the precursor may have escaped detection. Therefore, we performed a pulse-chase experiment as described above for Kar2p. All of the labeled Hsp60 was already processed into the mature form at the end of the pulse (Fig. 9, lanes 4 and 7); precursor was not detectable at any time. The results were identical for wild-type and Δdjp1cells, indicating rapid import and processing of mitochondrial proteins in the absence of Djp1p.
Import into neither the ER nor mitochondria was affected in Δdjp1 cells. A common feature of proteins targeted to the ER and mitochondria is the NH2-terminal location of the organellar targeting sequence (signal sequence). Furthermore, signal-sequence–containing proteins are kept in a (partially) unfolded conformation during the translocation process. Since most peroxisomal matrix proteins contain a COOH-terminal targeting signal sequence, and since for at least two of our reporter proteins (Mdh3p and thiolase) it had been shown that they oligomerize before they are imported into the organelle, we also tested whether import into the nucleus of (folded) GFP fused to a nuclear localization signal was affected in Δdjp1 cells. In wild-type and Δdjp1 cells NLS-GFP accumulated in the nucleus (Fig. 10). No morphological abnormalities of the nucleus were observed. To study nuclear protein import kinetics, we made use of a recently published method (Shulga et al., 1996) which is based on the reversible import of NLS-GFP. Cells were depleted of ATP by incubating them with azide. This resulted in disappearance of the bright nuclear fluorescence and equilibration of NLS-GFP between cytosol and nucleus (Fig. 10). The vacuole remained unlabeled and appeared as a dark hole surrounded by a diffuse fluorescence. Upon restoration of import conditions (washing azide-treated cells and addition of glucose-containing medium), NLS-GFP concentrated into a small bright spot in each cell, reminiscent of the nucleus. Relative import rates were quantified by counting the percentage of cells that showed NLS-GFP nuclear accumulation as a function of time. Δdjp1 cells imported NLS-GFP with the same kinetics as wild-type cells. This experiment illustrated that Djp1p was not required for import of proteins into the nucleus.
Finally, we studied the rate at which Δdjp1 cells can adapt their metabolism to environmental changes, which depends on the synthesis and maturation of a number of (cytosolic) proteins. For this purpose exponentially growing cells were shifted from glucose medium to galactose medium and growth was measured. Δdjp1 cells grew at the same exponential rate as wild-type cells (Fig. 11), implying that under these conditions Djp1p was not required. Furthermore, Δdjp1 cells rapidly adapted to growth on galactose medium as the lag phase was similar to that of wild-type cells. To follow maturation of one protein in more detail, the induction of one of the enzymes specifically required for growth on galactose (galactose-1-phosphate uridylyltransferase [Gal-1-PUT]) was measured. In Δdjp1 cells this enzyme was induced with the same kinetics as in wild-type cells (Fig. 11). Metabolic adaptation requires the function of many processes, including signal transduction, de novo protein synthesis, folding, and protein sorting. We concluded from our results that Djp1p was specifically required for peroxisomal protein import and was not needed for maturation or import of proteins in any other organelle. Djp1p was therefore dispensible for cell growth under all culture conditions except oleate.
Transport of proteins across intracellular membranes requires molecular chaperones of the Hsp70 family. Hsp70 activity is regulated by J-domain–containing proteins that recruit Hsp70 to their site of action. The J-domain recruits a specific Hsp70 family member whereas other parts of the J-domain–containing protein are thought to interact with the Hsp70 substrate (Rassow et al., 1995). Cytosolic Hsp70s and J-domain–containing proteins are involved in posttranslational translocation of proteins across the ER and mitochondrial membranes, presumably by keeping precursor proteins in a partially unfolded translocation-competent conformation (for review see Brodsky and Schekman, 1994; Mihara and Omura, 1996; Rassow et al., 1997). Luminal organellar Hsp70s are required for both the translocation process and the folding and assembly of proteins (Hartl, 1996).
Whereas ER and mitochondrial proteins do not acquire their native states until translocation is complete, nuclear and peroxisomal proteins can fold and oligomerize before import. Targeting and translocation of nuclear proteins requires Hsp70. In a semi-intact cell system, import into peroxisomes of microinjected human serum albumin coated with multiple PTS1-containing peptides was shown to require cytosolic Hsp73 (Walton et al., 1994). A prenylated DnaJ homologue has been found on the cytoplasmic side of cucumber glyoxysomes, although the significance of this association remains to be established (Preisig-Mueller et al., 1994). The specific defect of cells lacking the J-domain– containing protein Djp1p provides the first genetic evidence that molecular chaperones are required for efficient peroxisomal protein import in vivo.
Djp1p behaved as a mostly cytosolic protein. It is easy to envisage why J-domain–containing proteins located inside an organelle are involved in the import of proteins specifically into that organelle. It is surprising though that a largely cytosolic protein such as Djp1p is specific for peroxisomal protein import, and is not required for import of proteins into any other organelle. Still, our data clearly indicate that Djp1p functions in an organelle-specific manner. In Δdjp1 cells peroxisomal structures are small and of intermediate buoyant density, resembling the early stages of peroxisome proliferation in S. cerevisiae (Erdmann and Blobel, 1995; our own unpublished observations). This slight organelle maturation defect accompanied the partial mislocalization of peroxisomal matrix proteins to the cytosol, which suggested that the primary defect in Δdjp1 cells is in the import of peroxisomal proteins. Kinetic studies, performed under various growth conditions, revealed a lower import rate of all tested peroxisomal matrix proteins in Δdjp1 cells (Ruigrok, C., manuscript in preparation).
When considering the function of Djp1p and its cognate Hsp70 partner in the import of peroxisomal proteins, several roles come to mind, each analogous to the role of another chaperone. Newly synthesized proteins are guided from ribosomes to their specific subcellular location through a series of interactions with cellular proteins. One of the first is the association with soluble targeting factors. Recognition of mitochondrial and ER presequences occurs at the ribosome (for review see Bukau et al., 1996). On the other hand, most peroxisomal matrix proteins contain a PTS1, which is found at the extreme COOH terminus. Since addition of the PTS1 to GFP was sufficient to direct it to peroxisomes, specific recognition of the targeting signal in GFP-PTS1 could occur only after translation termination. Because GFP-PTS1 was partially mislocalized in Δdjp1 cells, Djp1p must function mainly after completion of translation.
Our experiments indicated that Djp1p has a stimulatory effect on peroxisomal protein import. Its effect is essential, however, for peroxisome biogenesis; it ensures sufficient import to maintain the function of peroxisomes. It is unlikely that Djp1p plays a role in the posttranslational folding and assembly of peroxisomal proteins since mislocalized peroxisomal enzymes are active in the cytosol of Δdjp1 cells. In addition, there is no requirement of a native protein conformation for import into peroxisomes (Walton et al., 1992, 1995), and human alanine:glyoxylate aminotransferase 1 (AGT) can be imported either as a monomer or as a dimer (Leiper et al., 1996).
Djp1p and its putative Hsp70 partner could keep peroxisomal proteins in a translocation-competent conformation. Whereas the subunits in oligomeric proteins have been shown to stay together during peroxisomal import (Glover et al., 1994; McNew and Goodman 1994; Elgersma et al., 1996b; Leiper et al., 1996), the actual conformation during translocation is unknown. Djp1p may be needed to keep the newly synthesized proteins in a flexible, extended conformation, which nonetheless allows oligomeric contacts. Analogously, the cytosolic Hsp70 chaperone Ssa has been proposed to stimulate posttranslational import of certain newly synthesized ER and mitochondrial preproteins by keeping the precursors in an incompletely folded import-competent conformation (for review see Brodsky and Schekman, 1994; Corsi and Schekman, 1996; Mihara and Omura, 1996). Ssa collaborates in this process with the J-domain–containing protein Ydj1p (Becker et al., 1996). The exact function of Ydj1p has not been described.
As a putative cofactor to a molecular chaperone, Djp1p does not need to be involved in a folding process. DnaJ-like proteins have been reported to assist in modulating protein–protein interactions of already folded proteins as well. It is possible that Djp1p stimulates recognition of peroxisomal proteins by the PTS receptors, thereby increasing targeting fidelity. Djp1p may act on newly synthesized peroxisomal matrix proteins as well as on PTS receptors, modifying their conformation by associating with either protein or both. If Djp1p presents GFP-PTS1 to its receptor (Pex5p), the only way Djp1p could recognize GFP-PTS1 is via its PTS1. Experimental data to support a direct or indirect interaction between the PTS1 and Djp1p do not exist. A precedent for Hsp70-mediated presentation of targeting signals to their cognate receptors, however, has been found in nuclear protein import (Melchior and Gerace, 1995).
Another step in the maturation of peroxisomal proteins is their targeting to and entry into the organelle. In this step, Djp1p and its cognate Hsp70 may regulate the activity of the PTS receptors in a manner similar to the way Hsp70 and Hsp40 regulate, in concert with additional chaperones, the activity of hormone receptors (Bohen et al., 1995). If Djp1p indeed is involved in the regulation of PTS receptor docking onto the peroxisome, its absence would cause permanent shielding or permanent exposure of docking interaction domains in the receptors, the former resulting in a lack of interaction of PTS receptors with the peroxisome, the latter leading to futile docking and release cycles of empty receptor molecules. Both phenotypes correspond well with that of the Δdjp1 cells, in which peroxisomal protein import is impaired. The same phenotype would arise if a Djp1p/Hsp70 complex plays a role in conformational changes required to convert released empty PTS receptor into a form that can bind PTS-containing proteins again. Hsp70 / Hsp40 have indeed been shown to be involved in increasing the availability of ligand-responsive aporeceptor (Frydman and Höhfeld, 1997).
After docking of PTS receptor charged with a cargo PTS protein, a complex series of events is likely to occur during the translocation process. At least three peroxisomal membrane proteins (Pex13p, Pex14p, and Pex17p [formerly Pas9p]) bind the PTS1 receptor, possibly in a cascade of sequential interactions with peroxins at the peroxisomal membrane (Elgersma and Tabak, 1996; Albertini et al., 1997). It is conceivable that Djp1p/Hsp70 functions as a modulator for the various protein–protein interactions, stimulating successive binding and release.
Since Djp1p is required for import of both PTS1- and PTS2-containing proteins, Djp1p most likely acts either at the point of or after convergence of the two pathways. Since the only PTS-specific import factors identified thus far are the PTS receptors, the two pathways might merge soon after recognition of the PTS-containing proteins by their cognate receptors. In S. cerevisiae, the PTS1 receptor and PTS2 receptor interact in vivo, thereby forming a receptor complex that may be responsible for the recognition or targeting of both PTS1- and PTS2-containing proteins simultaneously (Rehling et al., 1996; Albertini et al., 1997; Huhse et al., 1998). Djp1p may stimulate the formation of this complex. Lack of one of the receptors, however, does not abolish the function of the other receptor, indicating that formation of this putative cytoplasmic targeting complex is not a prerequisite for targeting. Since kinetic studies on import of PTS2-containing proteins in PTS1 receptor–deficient yeast cells or vice versa have never been done, we cannot exclude that both receptors are necessary for a high efficiency of peroxisomal matrix protein import. Indeed, PTS2 import in mammalian cells is dependent on the presence of the PTS1 receptor (Dodt et al., 1995; Wiemer et al., 1995; Baes et al., 1997; Otera et al., 1998). Alternatively, Djp1p may modulate the various protein–protein interactions required during the targeting cycle of this dual receptor complex in a way similar to that discussed for the single PTS receptors.
Kinetic studies in mammalian and yeast cells have revealed that some peroxisomal proteins initially associate with low density organellar structures (“pre-peroxisomes”) before reaching the mature peroxisomes of high density (Heinemann and Just, 1992; Luers et al., 1993; Titerenko and Rachubinski, 1998). Intriguingly, we observed that Djp1p is involved in peroxisomal protein import and is associated with low density membrane structures distinct from peroxisomes. Together these results indicate that Djp1p may play a role in the import process of proteins into precursor peroxisomes.
To summarize, we have found the first peroxisome-specific cofactor to a chaperone: Djp1p. Our genetic and biochemical evidence clearly shows that Djp1p is involved in the import of peroxisomal proteins only, and not in processes related to other organelles. Surprisingly, despite its specificity for peroxisomes, Djp1p is located mainly in the cytosol with a minor fraction associated with membranes, which opens up a wealth of possible roles that the protein may play. For every possible scenario outlined above, a precedent exists in the form of a known Hsp70 chaperone complex. Our results are clear but do not yet allow us to distinguish between these different possible functions of Djp1p. A search for the partner protein(s) of Djp1p is in progress, and with the Δdjp1 mutant available, we have started to investigate the precise role of molecular chaperones in the peroxisomal protein import process.
We are grateful to Drs. J. Goodman and M. Meijer for the Pex11p and F1β-ATPase antibodies, respectively; Dr. A. Hartig for the reagents to produce CTA antiserum; and Dr. S. Gould for GFP-PTS constructs. We thank Dr. S. Rospert for the Δtom20 mutant and anti-Hsp60 antiserum. We thank Dr. R. Schekman for the Sec62ts mutant and Dr. M. Rose for reagents to produce Kar2p antiserum. We thank Dr. A. Kal and T. Voorn-Brouwer for production of antisera against Kar2p and thiolase, and Dr. Y. Elgersma for isolation of the pas22-1 mutant. We thank C. van Roermund for assistance with the Nycodenz density gradients; C. Dekker, C. van Roermund, and Dr. R. Wanders for the Gal-1-PUT measurements; and W. de Jonge for technical assistance with the mitochondrial pulse-chase experiment. We thank Dr. A. Motley for critically reading the manuscript.
This work was supported by a grant from the Netherlands Organization for Scientific Research (NWO) to H.F. Tabak and from the Royal Netherlands Academy of Arts and Sciences to I. Braakman.
Abbreviations used in this paper
DnaJ-like protein required for peroxisomal protein import
green fluorescent protein
NH2-terminal hemagglutinin epitope tag
nuclear localization signal
open reading frame
peroxisome targeting signal
E.H. Hettema and C.C.M. Ruigrok contributed equally to this paper.
Address all correspondence to I. Braakman, Department of Biochemistry, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands. Tel.: +31-20-566-3297. Fax +31-20-691-5519. E-mail: firstname.lastname@example.org