Multinucleated skeletal muscle fibers synthesize cell surface and secreted oligomeric forms of acetylcholinesterase (AChE) that accumulate at specialized locations on the cell surface, such as sites of nerve-muscle contact. Using allelic variants of the AChE polypeptide chains as genetic markers, we show that nuclei homozygous for either the alpha or beta alleles residing in chimeric myotubes preferentially translate their AChE mRNAs on their respective ERs. These results indicate that the events of transcription, translation, and assembly of this membrane protein are compartmentalized into nuclear domains in multinucleated cells, and provide the structural basis for the possible localized expression and regulation of synaptic components at the neuromuscular junctions of vertebrate skeletal muscle fibers.
Article| March 01 1990
Nucleus-specific translation and assembly of acetylcholinesterase in multinucleated muscle cells.
R L Rotundo
Department of Cell Biology and Anatomy, University of Miami School of Medicine, Florida 33101.
Online Issn: 1540-8140
Print Issn: 0021-9525
J Cell Biol (1990) 110 (3): 715–719.
- Views Icon Views
- Share Icon Share
- Search Site
R L Rotundo; Nucleus-specific translation and assembly of acetylcholinesterase in multinucleated muscle cells.. J Cell Biol 1 March 1990; 110 (3): 715–719. doi: https://doi.org/10.1083/jcb.110.3.715
Download citation file: