We have investigated the contribution of an individual nucleus to intracellular and surface membranes in multinucleated muscle fibers. Using a retroviral vector, we introduced the gene encoding the human T-lymphocyte antigen CD8 into C2 mouse muscle cells to form a stable line expressing the human protein on its surface. The intracellular and surface distributions of the protein were then investigated by immunocytochemistry in hybrid myotubes containing a single nucleus expressing CD8. We show that the intracellular distribution of CD8 is limited to a local area surrounding the nucleus encoding it and several neighboring nuclei. On the cell surface, however, the protein is distributed over the entire myotube. Widespread distribution of a surface membrane protein in multinucleated myotubes can thus result from localized synthesis and processing.
Intracellular and surface distribution of a membrane protein (CD8) derived from a single nucleus in multinucleated myotubes.
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E Ralston, Z W Hall; Intracellular and surface distribution of a membrane protein (CD8) derived from a single nucleus in multinucleated myotubes.. J Cell Biol 1 November 1989; 109 (5): 2345–2352. doi: https://doi.org/10.1083/jcb.109.5.2345
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