Mammalian metaphase chromosomes can be identified by their characteristic banding pattern when stained with Giemsa dye after brief proteolytic digestion. The resulting G-bands are known to contain regions of DNA enriched in A/T residues and to be the principal location for the L1 (or Kpn 1) family of long interspersed repetitive sequences in human chromosomes. Here we report that antibodies raised against a highly purified and biochemically well characterized nonhistone "High-Mobility Group" protein, HMG-I, specifically localize this protein to the G-bands in mammalian metaphase chromosomes. In some preparations in which chromosomes are highly condensed, HMG-I appears to be located at the centromere and/or telomere regions of mammalian chromosomes as well. To our knowledge, this is the first well-characterized mammalian protein that localizes primarily to G-band regions of chromosomes.
High-mobility group protein HMG-I localizes to G/Q- and C-bands of human and mouse chromosomes.
J E Disney, K R Johnson, N S Magnuson, S R Sylvester, R Reeves; High-mobility group protein HMG-I localizes to G/Q- and C-bands of human and mouse chromosomes.. J Cell Biol 1 November 1989; 109 (5): 1975–1982. doi: https://doi.org/10.1083/jcb.109.5.1975
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