Cytoplasmic extracts of meiotically mature mouse oocytes were injected into immature Xenopus laevis oocytes, which underwent germinal vesicle breakdown within 2 h. Germinal vesicle breakdown was not inhibited by incubation of the Xenopus oocytes in cycloheximide (20 micrograms/ml). Identically prepared extracts of meiotically immature mouse oocytes, arrested at the germinal vesicle stage by dibutyryl cyclic AMP (100 micrograms/ml), did not induce germinal vesicle breakdown in Xenopus oocytes. The results show that maturation-promoting factor activity appears during the course of oocyte maturation in the mouse.
Article| May 01 1985
Active maturation-promoting factor is present in mature mouse oocytes.
R A Sorensen
M S Cyert
R A Pedersen
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1985) 100 (5): 1637–1640.
R A Sorensen, M S Cyert, R A Pedersen; Active maturation-promoting factor is present in mature mouse oocytes.. J Cell Biol 1 May 1985; 100 (5): 1637–1640. doi: https://doi.org/10.1083/jcb.100.5.1637
Download citation file: