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Volume 1, Issue 5
25 September 1955
Content prior to 1962 was published under the journal name
The Journal of Biophysical and Biochemical Cytology
The Journal of Biophysical and Biochemical Cytology Cover Image for Volume 1, Issue 5
Article| September 25 1955

BUFFERED OSMIUM TETROXIDE (OsO4) FIXATION FOR CYTOLOGICAL AND FEULGEN MICROSPECTROPHOTOMETRIC STUDIES OF HUMAN RECTAL POLYPS

Cecilie Leuchtenberger,
Cecilie Leuchtenberger
(From the Institute of Pathology, Western Reserve University, Cleveland, Ohio)
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Paul F. Doolin,
Paul F. Doolin
(From the Institute of Pathology, Western Reserve University, Cleveland, Ohio)
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Anne H. Kutsakis
Anne H. Kutsakis
(From the Institute of Pathology, Western Reserve University, Cleveland, Ohio)
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Author and Article Information
Cecilie Leuchtenberger
(From the Institute of Pathology, Western Reserve University, Cleveland, Ohio)
Paul F. Doolin
(From the Institute of Pathology, Western Reserve University, Cleveland, Ohio)
Anne H. Kutsakis
(From the Institute of Pathology, Western Reserve University, Cleveland, Ohio)
Received: June 08 1955
Copyright, 1955, by The Rockefeller Institute for Medical Research
1955
J Biophys and Biochem Cytol (1955) 1 (5): 385–390.
https://doi.org/10.1083/jcb.1.5.385
Article history
Received:
June 08 1955

Evidence has been presented that when human rectal mucosa and polyps are fixed with buffered OsO4 according to the method of Palade, staining of paraffin sections by basic and acidic dyes results in a distinct color difference between chromatin and cytoplasm. Furthermore, the Feulgen reaction also can be carried out successfully and such OsO4-fixed Feulgen-stained sections are especially suitable for microspectrophotometric determinations of DNA in individual nuclei.

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Copyright, 1955, by The Rockefeller Institute for Medical Research
1955
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