A combined physiological and morphological study of the effects of cytochalasin B (CB) on the toad urinary bladder has been carried out. CB inhibits the hydro-osmotic response to vasopressin without altering basal water permeability or diffusion, or the increase in 3H2O diffusion observed after hormone addition. Although CB increases [22Na]-, [36Cl]-, and [14C]urea fluxes, and decreases transepithelial potential, no alteration in basal short-circuit current, the vasopressin-induced increase in this parameter, or [14C]inulin permeability occurs. In the absence of hormone, CB does not markedly alter the structure of the toad bladder. However, in the presence of vasopressin, CB induces the formation of large intracellular vacuoles. These results suggest a possible coupling of solute and water movement across the tissue.
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1 December 1974
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December 01 1974
EFFECTS OF CYTOCHALASIN B ON THE RESPONSE OF TOAD URINARY BLADDER TO VASOPRESSIN
Walter L. Davis,
Walter L. Davis
From the Departments of Biochemistry and Pediatrics, University of Pennsylvania Medical School, Philadelphia, Pennsylvania 19174, and the Department of Pathology, Baylor University Medical Center, Dallas, Texas 75226
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David B. P. Goodman,
David B. P. Goodman
From the Departments of Biochemistry and Pediatrics, University of Pennsylvania Medical School, Philadelphia, Pennsylvania 19174, and the Department of Pathology, Baylor University Medical Center, Dallas, Texas 75226
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Richard J. Schuster,
Richard J. Schuster
From the Departments of Biochemistry and Pediatrics, University of Pennsylvania Medical School, Philadelphia, Pennsylvania 19174, and the Department of Pathology, Baylor University Medical Center, Dallas, Texas 75226
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Howard Rasmussen,
Howard Rasmussen
From the Departments of Biochemistry and Pediatrics, University of Pennsylvania Medical School, Philadelphia, Pennsylvania 19174, and the Department of Pathology, Baylor University Medical Center, Dallas, Texas 75226
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James H. Martin
James H. Martin
From the Departments of Biochemistry and Pediatrics, University of Pennsylvania Medical School, Philadelphia, Pennsylvania 19174, and the Department of Pathology, Baylor University Medical Center, Dallas, Texas 75226
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Walter L. Davis
From the Departments of Biochemistry and Pediatrics, University of Pennsylvania Medical School, Philadelphia, Pennsylvania 19174, and the Department of Pathology, Baylor University Medical Center, Dallas, Texas 75226
David B. P. Goodman
From the Departments of Biochemistry and Pediatrics, University of Pennsylvania Medical School, Philadelphia, Pennsylvania 19174, and the Department of Pathology, Baylor University Medical Center, Dallas, Texas 75226
Richard J. Schuster
From the Departments of Biochemistry and Pediatrics, University of Pennsylvania Medical School, Philadelphia, Pennsylvania 19174, and the Department of Pathology, Baylor University Medical Center, Dallas, Texas 75226
Howard Rasmussen
From the Departments of Biochemistry and Pediatrics, University of Pennsylvania Medical School, Philadelphia, Pennsylvania 19174, and the Department of Pathology, Baylor University Medical Center, Dallas, Texas 75226
James H. Martin
From the Departments of Biochemistry and Pediatrics, University of Pennsylvania Medical School, Philadelphia, Pennsylvania 19174, and the Department of Pathology, Baylor University Medical Center, Dallas, Texas 75226
Received:
April 01 1974
Revision Received:
June 25 1974
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright © 1974 by The Rockefeller University Press
1974
J Cell Biol (1974) 63 (3): 986–997.
Article history
Received:
April 01 1974
Revision Received:
June 25 1974
Citation
Walter L. Davis, David B. P. Goodman, Richard J. Schuster, Howard Rasmussen, James H. Martin; EFFECTS OF CYTOCHALASIN B ON THE RESPONSE OF TOAD URINARY BLADDER TO VASOPRESSIN . J Cell Biol 1 December 1974; 63 (3): 986–997. doi: https://doi.org/10.1083/jcb.63.3.986
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