CHEMICAL CHARACTERIZATION OF THE ISOLATED CELL SURFACE OF AMOEBA

Allen , Howard J.; Ault , Charles; Winzler , Richard J.; Danielli , James F.

doi:10.1083/jcb.60.1.26

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Article| January 01 1974

CHEMICAL CHARACTERIZATION OF THE ISOLATED CELL SURFACE OF AMOEBA

Howard J. Allen,

Howard J. Allen

From the Department of Chemistry, Florida State University, Tallahassee, Florida 32306 and the Center for Theoretical Biology, State University of New York at Buffalo, Buffalo, New York 14214.

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Charles Ault,

Charles Ault

From the Department of Chemistry, Florida State University, Tallahassee, Florida 32306 and the Center for Theoretical Biology, State University of New York at Buffalo, Buffalo, New York 14214.

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Richard J. Winzler,

Richard J. Winzler

From the Department of Chemistry, Florida State University, Tallahassee, Florida 32306 and the Center for Theoretical Biology, State University of New York at Buffalo, Buffalo, New York 14214.

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James F. Danielli

From the Department of Chemistry, Florida State University, Tallahassee, Florida 32306 and the Center for Theoretical Biology, State University of New York at Buffalo, Buffalo, New York 14214.

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Author and Article Information

Howard J. Allen

From the Department of Chemistry, Florida State University, Tallahassee, Florida 32306 and the Center for Theoretical Biology, State University of New York at Buffalo, Buffalo, New York 14214.

Charles Ault

From the Department of Chemistry, Florida State University, Tallahassee, Florida 32306 and the Center for Theoretical Biology, State University of New York at Buffalo, Buffalo, New York 14214.

Richard J. Winzler

From the Department of Chemistry, Florida State University, Tallahassee, Florida 32306 and the Center for Theoretical Biology, State University of New York at Buffalo, Buffalo, New York 14214.

James F. Danielli

From the Department of Chemistry, Florida State University, Tallahassee, Florida 32306 and the Center for Theoretical Biology, State University of New York at Buffalo, Buffalo, New York 14214.

Dr. Alien's present address is the Department of Gynecology, Boswell Park Memorial Institute, Buffalo, New York 14203.

Received: November 27 1972

Revision Received: August 20 1973

Online ISSN: 1540-8140

Print ISSN: 0021-9525

1974

J Cell Biol (1974) 60 (1): 26–38.

https://doi.org/10.1083/jcb.60.1.26

The cell surface has been isolated from uninucleate, freshwater, phagocytic amoebae by a new procedure. Several criteria were employed to demonstrate purity of the cell surface fraction. All morphological components of the tripartite surface were present in the isolated surface and the weight of the isolated surface was quantitatively accounted for by the components analyzed. Chemical analyses showed the presence of lipid, protein, and carbohydrate. Mannose was the predominant neutral sugar. Analyses for three different strains of Amoeba were similar. Phosphate was found to be the major anionic group in the cell surface material. Sulfate, uronic acid, sialic acid, muramic acid, and nonamidated glutamic acid and aspartic acid were absent. Evidence is presented suggesting that the phosphate is associated with an unidentified nonreducing polyol.

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