Eosinophil (and heterophil) leukocytes of glycogen-induced rabbit peritoneal exudates were fixed for 1½ min in 2% glutaraldehyde and examined for acid phosphatase activity both biochemically and cytochemically. Biochemical assays showed that enzymatic activity had been inhibited by only ∼10% under these conditions. The cytochemical reaction in the eosinophil was confined to the granules in which the reaction product appeared in the matrix, not in the crystalline core (or in the core region after the latter's extraction). Granules wherein the matrix was disrupted and the crystalline core degraded or extracted showed the most intense deposition of reaction product, whereas well preserved granules with morphologically intact matrix and crystals were unreactive. Yet, not all disrupted granules gave a positive reaction, indicating that disruption was a necessary but not sufficient condition for reactivity. In many eosinophil leukocytes, most if not all granules were acid phosphatase-positive, provided they had become disrupted to a certain degree. Factors possibly involved in converting the granules from an unreactive to a reactive state are discussed.
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1 September 1967
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September 01 1967
ACID PHOSPHATASE LOCALIZATION IN RABBIT EOSINOPHILS
Philip M. Seeman,
Philip M. Seeman
From The Rockefeller University, New York, New York 10021.
Search for other works by this author on:
George E. Palade
George E. Palade
From The Rockefeller University, New York, New York 10021.
Search for other works by this author on:
Philip M. Seeman
From The Rockefeller University, New York, New York 10021.
George E. Palade
From The Rockefeller University, New York, New York 10021.
Dr. Seeman's present address is the Department of Pharmacology, University of Toronto, Toronto, Canada
Received:
January 25 1967
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright © 1967 by The Rockefeller University Press
1967
J Cell Biol (1967) 34 (3): 745–756.
Article history
Received:
January 25 1967
Citation
Philip M. Seeman, George E. Palade; ACID PHOSPHATASE LOCALIZATION IN RABBIT EOSINOPHILS . J Cell Biol 1 September 1967; 34 (3): 745–756. doi: https://doi.org/10.1083/jcb.34.3.745
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