An electron microscope study has been made of vitally stained single cells whose cytoplasm has been subjected to a localized ruby laser microbeam. Light and moderate laser absorption (the resultant of stain concentration and laser energy density) produced restricted selective damage of mitochondria in cells stained with Janus green B; heavy laser absorption resulted in mitochondrial damage, as well as in nonselective interaction with other cell structures. With four other basic vital stains, the polysomes, ergastoplasm, mitochondria and other organelles at the irradiated site were uniformly damaged. Unstained cells showed no morphological alterations. With light primary damage (that restricted to the irradiation site), no secondary effects of the incident radiation were observed. With moderate primary damage, however, secondary damage of the mitochondria in the unirradiated cell portions was produced, which was reversible within 4 hr after irradiation. Heavy primary lesions caused severe secondary alteration of all cell structures that was irreversible and cell death occurred within 2 hr. Surviving cells examined 24 hr after light and moderate irradiation could not be distinguished from unirradiated controls. The possible mechanisms involved in the production of laser-induced cellular alterations are discussed.
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1 October 1966
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October 01 1966
AN ELECTRON MICROSCOPE STUDY OF VITALLY STAINED SINGLE CELLS IRRADIATED WITH A RUBY LASER MICROBEAM
Rainer Storb,
Rainer Storb
From Centre National de Transfusion Sanguine, Paris, France
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Robert L. Amy,
Robert L. Amy
From Centre National de Transfusion Sanguine, Paris, France
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Richard K. Wertz,
Richard K. Wertz
From Centre National de Transfusion Sanguine, Paris, France
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Bernard Fauconnier,
Bernard Fauconnier
From Centre National de Transfusion Sanguine, Paris, France
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Marcel Bessis
Marcel Bessis
From Centre National de Transfusion Sanguine, Paris, France
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Rainer Storb
From Centre National de Transfusion Sanguine, Paris, France
Robert L. Amy
From Centre National de Transfusion Sanguine, Paris, France
Richard K. Wertz
From Centre National de Transfusion Sanguine, Paris, France
Bernard Fauconnier
From Centre National de Transfusion Sanguine, Paris, France
Marcel Bessis
From Centre National de Transfusion Sanguine, Paris, France
Received:
March 29 1966
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright © 1966 by The Rockefeller University Press
1966
J Cell Biol (1966) 31 (1): 11–29.
Article history
Received:
March 29 1966
Citation
Rainer Storb, Robert L. Amy, Richard K. Wertz, Bernard Fauconnier, Marcel Bessis; AN ELECTRON MICROSCOPE STUDY OF VITALLY STAINED SINGLE CELLS IRRADIATED WITH A RUBY LASER MICROBEAM . J Cell Biol 1 October 1966; 31 (1): 11–29. doi: https://doi.org/10.1083/jcb.31.1.11
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