Small granules with a diameter of approximately 350 A are attached to the chloroplast lamellae of the red alga Porphyridium cruentum. To some extent, their size depends on the culture conditions and the age of the cell. It was possible to preserve the granules only with aldehyde prefixation. It can be seen that fixed or negatively stained granules are comprised of smaller subunits. The granules are arranged regularly on the lamellae in repeating rows with a center-to-center granule distance of 400 to 500 A. Attempts at characterization of these chloroplast granules revealed that they are resistant to hydrolysis by ribonuclease and appear to be structurally unaffected by methanol-acetone extraction. Because of their close association with the chloroplast lamellae, they are considered as possible sites of phycobilin concentration. This possibility is supported by two observations: when the phycobilins are removed, the granules disappear; and, when the chlorophyll and stainable membrane portions are selectively removed, the phycobilins and granules are still present. It was found that all other marine red algae examined had granules which were associated with the chloroplast lamellae.
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1 June 1966
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June 01 1966
GRANULES ASSOCIATED WITH THE CHLOROPLAST LAMELLAE OF PORPHYRIDIUM CRUENTUM
E. Gantt,
E. Gantt
From the Department of Microbiology, Dartmouth Medical School, Hanover, New Hampshire
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S. F. Conti
S. F. Conti
From the Department of Microbiology, Dartmouth Medical School, Hanover, New Hampshire
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E. Gantt
From the Department of Microbiology, Dartmouth Medical School, Hanover, New Hampshire
S. F. Conti
From the Department of Microbiology, Dartmouth Medical School, Hanover, New Hampshire
Received:
January 27 1966
Online ISSN: 1540-8140
Print ISSN: 0021-9525
1966
J Cell Biol (1966) 29 (3): 423–434.
Article history
Received:
January 27 1966
Citation
E. Gantt, S. F. Conti; GRANULES ASSOCIATED WITH THE CHLOROPLAST LAMELLAE OF PORPHYRIDIUM CRUENTUM . J Cell Biol 1 June 1966; 29 (3): 423–434. doi: https://doi.org/10.1083/jcb.29.3.423
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