Primary cilia are sensory structures involved in morphogen signalling during development, liquid flow in the kidney, mechanosensation, sight, and smell (Badano, J.L., N. Mitsuma, P.L. Beales, and N. Katsanis. 2006. Annu. Rev. Genomics Hum. Genet. 7:125–148; Singla, V., and J.F. Reiter. 2006. Science. 313:629–633.). Mutations that affect primary cilia are responsible for several diseases, including neural tube defects, polycystic kidney disease, retinal degeneration, and cancers (Badano et al., 2006; Singla and Reiter, 2006). Primary cilia formation and function requires tight integration of the microtubule cytoskeleton with membrane trafficking (Singla and Reiter, 2006), and this is poorly understood. We show that the Rab GTPase membrane trafficking regulators Rab8a, -17, and -23, and their cognate GTPase-activating proteins (GAPs), XM_037557, TBC1D7, and EVI5like, are involved in primary cilia formation. However, other human Rabs and GAPs are not. Additionally, Rab8a specifically interacts with cenexin/ODF2, a basal body and microtubule binding protein required for cilium biogenesis (Ishikawa, H., A. Kubo, S. Tsukita, and S. Tsukita. 2005. Nat. Cell Biol. 7:517–524), and is the sole Rab enriched at primary cilia. These findings provide a basis for understanding how specific membrane trafficking pathways cooperate with the microtubule cytoskeleton to give rise to the primary cilia.
Skip Nav Destination
Article navigation
30 July 2007
Report|
July 23 2007
Functional dissection of Rab GTPases involved in primary cilium formation
Shin-ichiro Yoshimura,
Shin-ichiro Yoshimura
1University of Liverpool Cancer Research Centre, Liverpool L3 9TA, England, UK
2Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried, 82152 Germany
Search for other works by this author on:
Johannes Egerer,
Johannes Egerer
2Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried, 82152 Germany
Search for other works by this author on:
Evelyn Fuchs,
Evelyn Fuchs
2Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried, 82152 Germany
Search for other works by this author on:
Alexander K. Haas,
Alexander K. Haas
2Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried, 82152 Germany
Search for other works by this author on:
Francis A. Barr
Francis A. Barr
1University of Liverpool Cancer Research Centre, Liverpool L3 9TA, England, UK
2Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried, 82152 Germany
Search for other works by this author on:
Shin-ichiro Yoshimura
1University of Liverpool Cancer Research Centre, Liverpool L3 9TA, England, UK
2Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried, 82152 Germany
Johannes Egerer
2Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried, 82152 Germany
Evelyn Fuchs
2Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried, 82152 Germany
Alexander K. Haas
2Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried, 82152 Germany
Francis A. Barr
1University of Liverpool Cancer Research Centre, Liverpool L3 9TA, England, UK
2Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried, 82152 Germany
Correspondence to Francis Barr: [email protected]
Abbreviations used in this paper: GAP, GTPase-activating protein; IFT, intraflagellar transport complex.
Received:
March 08 2007
Accepted:
June 28 2007
Online ISSN: 1540-8140
Print ISSN: 0021-9525
The Rockefeller University Press
2007
J Cell Biol (2007) 178 (3): 363–369.
Article history
Received:
March 08 2007
Accepted:
June 28 2007
Citation
Shin-ichiro Yoshimura, Johannes Egerer, Evelyn Fuchs, Alexander K. Haas, Francis A. Barr; Functional dissection of Rab GTPases involved in primary cilium formation . J Cell Biol 30 July 2007; 178 (3): 363–369. doi: https://doi.org/10.1083/jcb.200703047
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionEmail alerts
Advertisement
Advertisement