Cell migration is essential for proper development of numerous structures derived from embryonic neural crest cells (NCCs). Although the migratory pathways of NCCs have been determined, the molecular mechanisms regulating NCC motility remain unclear. NCC migration is integrin dependent, and recent work has shown that surface expression levels of particular integrin α subunits are important determinants of NCC motility in vitro. Here, we provide evidence that rapid cranial NCC motility on laminin requires integrin recycling. NCCs showed both ligand- and receptor-specific integrin regulation in vitro. On laminin, NCCs accumulated internalized laminin but not fibronectin receptors over 20 min, whereas on fibronectin neither type of receptor accumulated internally beyond 2 min. Internalized laminin receptors colocalized with receptor recycling vesicles and were subsequently recycled back to the cell surface. Blocking receptor recycling with bafilomycin A inhibited NCC motility on laminin, indicating that substratum-dependent integrin recycling is essential for rapid cranial neural crest migration.
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8 November 2004
Article|
November 01 2004
Cranial neural crest recycle surface integrins in a substratum-dependent manner to promote rapid motility
Lauren R. Strachan,
Lauren R. Strachan
Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, UT 84132
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Maureen L. Condic
Maureen L. Condic
Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, UT 84132
Search for other works by this author on:
Lauren R. Strachan
Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, UT 84132
Maureen L. Condic
Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, UT 84132
Correspondence to Maureen Condic: [email protected]
Abbreviations used in this paper: BafA, bafilomycin A; FN1, fibronectin applied at 1 μg/ml (low fibronectin); FN20, fibronectin applied at 20 μg/ml (high fibronectin; LM1, laminin applied at 1 μg/ml (low laminin); LM20, laminin applied at 20 μg/ml (high laminin); MesNa, sodium 2-mercaptoethanesulfonate; NCC, neural crest cell.
Received:
May 04 2004
Accepted:
October 05 2004
Online ISSN: 1540-8140
Print ISSN: 0021-9525
The Rockefeller University Press
2004
J Cell Biol (2004) 167 (3): 545–554.
Article history
Received:
May 04 2004
Accepted:
October 05 2004
Citation
Lauren R. Strachan, Maureen L. Condic; Cranial neural crest recycle surface integrins in a substratum-dependent manner to promote rapid motility . J Cell Biol 8 November 2004; 167 (3): 545–554. doi: https://doi.org/10.1083/jcb.200405024
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