The YSK1 kinase (red) is brought to the Golgi by GM130 (green).
The authors find that the Golgi is home to two kinases, YSK1 and MST4. By binding to the Golgi matrix protein GM130, the kinases are autophosphorylated and thus activated. Mutants that prevented Golgi localization of activated YSK1 disrupted the normal perinuclear Golgi pattern. Some YSK1 substrates may therefore maintain Golgi structure.
Other substrates are needed for migration, even when the Golgi structure remains intact. The authors identified 14–3-3ζ as one YSK1 substrate whose phosphorylation was required for wound-induced cell migration. Overexpression of MST4, in contrast, blocked migration without altering Golgi structure. Migration could therefore be regulated by competition between the kinases for...
