Two channels, one program

The biophysical and structural characteristics of chloride ion channels have been studied extensively, but little is known about the molecular regulation and signaling pathways associated with these highly conserved proteins. Rutledge et al. (page 435) analyzed the activation of the CLH-3 chloride ion channel in C. elegans during meioitic cell cycle progression and in response to oocyte swelling. The work demonstrates the utility of the worm system in studying these channels, and suggests that CLH-3 and its putative mammalian orthologue, ClC-2, respond to similar regulatory inputs to carry out similar physiological functions.

The authors found that during oocyte maturation, or in response to oocyte swelling, CLH-3 is activated by serine/threonine dephosphorylation. RNAi inhibition demonstrates that the dephosphorylation is mediated by CeGLC-7α and CeGLC-7β, phosphatases that help to regulate meiotic and mitotic cell cycles in worms....