Intercellular adhesion molecule-5 (ICAM-5) is a dendritically polarized membrane glycoprotein in telencephalic neurons, which shows heterophilic binding to leukocyte β2-integrins. Here, we show that the human ICAM-5 protein interacts in a homophilic manner through the binding of the immunoglobulin domain 1 to domains 4–5. Surface coated ICAM-5-Fc promoted dendritic outgrowth and arborization of ICAM- 5–expressing hippocampal neurons. During dendritogenesis in developing rat brain, ICAM-5 was in monomer form, whereas in mature neurons it migrated as a high molecular weight complex. The findings indicate that its homophilic binding activity was regulated by nonmonomer/monomer transition. Thus, ICAM-5 displays two types of adhesion activity, homophilic binding between neurons and heterophilic binding between neurons and leukocytes.
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10 July 2000
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July 10 2000
Intercellular Adhesion Molecule-5 Induces Dendritic Outgrowth by Homophilic Adhesion
Li Tian,
Li Tian
aDepartment of Biosciences, Division of Biochemistry, Viikinkaari 5, University of Helsinki, Helsinki 00014, Finland
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Henrietta Nyman,
Henrietta Nyman
aDepartment of Biosciences, Division of Biochemistry, Viikinkaari 5, University of Helsinki, Helsinki 00014, Finland
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Patrick Kilgannon,
Patrick Kilgannon
bICOS Corporation, Bothell, Washington 98021
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Yoshihiro Yoshihara,
Yoshihiro Yoshihara
cLaboratory for Neurobiology of Synapse, Brain Science Institute, The Institute of Physical and Chemical Research (RIKEN), Wako-City, Saitama 351, Japan
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Kensaku Mori,
Kensaku Mori
dLaboratory for Neuronal Recognition Molecules, Brain Science Institute, RIKEN, Wako-City, Saitama 351, Japan
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Leif C. Andersson,
Leif C. Andersson
eHaartman Institute, Department of Pathology, University of Helsinki, Helsinki 00014, Finland
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Sami Kaukinen,
Sami Kaukinen
aDepartment of Biosciences, Division of Biochemistry, Viikinkaari 5, University of Helsinki, Helsinki 00014, Finland
fLaboratory of Molecular Neurobiology, Institute of Biotechnology, University of Helsinki, Helsinki 00014, Finland
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Heikki Rauvala,
Heikki Rauvala
aDepartment of Biosciences, Division of Biochemistry, Viikinkaari 5, University of Helsinki, Helsinki 00014, Finland
fLaboratory of Molecular Neurobiology, Institute of Biotechnology, University of Helsinki, Helsinki 00014, Finland
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W. Michael Gallatin,
W. Michael Gallatin
bICOS Corporation, Bothell, Washington 98021
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Carl G. Gahmberg
Carl G. Gahmberg
aDepartment of Biosciences, Division of Biochemistry, Viikinkaari 5, University of Helsinki, Helsinki 00014, Finland
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Li Tian
aDepartment of Biosciences, Division of Biochemistry, Viikinkaari 5, University of Helsinki, Helsinki 00014, Finland
Henrietta Nyman
aDepartment of Biosciences, Division of Biochemistry, Viikinkaari 5, University of Helsinki, Helsinki 00014, Finland
Patrick Kilgannon
bICOS Corporation, Bothell, Washington 98021
Yoshihiro Yoshihara
cLaboratory for Neurobiology of Synapse, Brain Science Institute, The Institute of Physical and Chemical Research (RIKEN), Wako-City, Saitama 351, Japan
Kensaku Mori
dLaboratory for Neuronal Recognition Molecules, Brain Science Institute, RIKEN, Wako-City, Saitama 351, Japan
Leif C. Andersson
eHaartman Institute, Department of Pathology, University of Helsinki, Helsinki 00014, Finland
Sami Kaukinen
aDepartment of Biosciences, Division of Biochemistry, Viikinkaari 5, University of Helsinki, Helsinki 00014, Finland
fLaboratory of Molecular Neurobiology, Institute of Biotechnology, University of Helsinki, Helsinki 00014, Finland
Heikki Rauvala
aDepartment of Biosciences, Division of Biochemistry, Viikinkaari 5, University of Helsinki, Helsinki 00014, Finland
fLaboratory of Molecular Neurobiology, Institute of Biotechnology, University of Helsinki, Helsinki 00014, Finland
W. Michael Gallatin
bICOS Corporation, Bothell, Washington 98021
Carl G. Gahmberg
aDepartment of Biosciences, Division of Biochemistry, Viikinkaari 5, University of Helsinki, Helsinki 00014, Finland
Abbreviations used in this paper: AxCAM, axon-associated cell adhesion molecule; D, immunoglobulin domain; DenCAM, dendrite-associated cell adhesion molecule; E19, embryonic day 19; ICAM, intercellular adhesion molecule; MAP-2, microtubule-associated protein-2; P, postnatal day; WT, wild-type.
Received:
November 29 1999
Revision Requested:
May 23 2000
Accepted:
May 25 2000
Online ISSN: 1540-8140
Print ISSN: 0021-9525
© 2000 The Rockefeller University Press
2000
The Rockefeller University Press
J Cell Biol (2000) 150 (1): 243–252.
Article history
Received:
November 29 1999
Revision Requested:
May 23 2000
Accepted:
May 25 2000
Citation
Li Tian, Henrietta Nyman, Patrick Kilgannon, Yoshihiro Yoshihara, Kensaku Mori, Leif C. Andersson, Sami Kaukinen, Heikki Rauvala, W. Michael Gallatin, Carl G. Gahmberg; Intercellular Adhesion Molecule-5 Induces Dendritic Outgrowth by Homophilic Adhesion. J Cell Biol 10 July 2000; 150 (1): 243–252. doi: https://doi.org/10.1083/jcb.150.1.243
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