Chicken acidic leucine-rich EGF-like domain containing brain protein (CALEB) was identified by combining binding assays with immunological screens in the chicken nervous system as a novel member of the EGF family of differentiation factors. cDNA cloning indicates that CALEB is a multidomain protein that consists of an NH2-terminal glycosylation region, a leucine-proline–rich segment, an acidic box, a single EGF-like domain, a transmembrane, and a short cytoplasmic stretch. In the developing nervous system, CALEB is associated with glial and neuronal surfaces. CALEB is composed of a 140/130-kD doublet, an 80-kD band, and a chondroitinsulfate-containing 200-kD component. The latter two components are expressed in the embryonic nervous system and are downregulated in the adult nervous system. CALEB binds to the extracellular matrix glycoproteins tenascin-C and -R. In vitro antibody perturbation experiments reveal a participation of CALEB in neurite formation in a permissive environment.
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24 February 1997
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February 24 1997
Chicken Acidic Leucine-rich EGF-like Domain Containing Brain Protein (CALEB), a Neural Member of the EGF Family of Differentiation Factors, Is Implicated in Neurite Formation
Stefan Schumacher,
Stefan Schumacher
*Max-Delbrück-Centrum für Molekulare Medizin, D-13122 Berlin, Germany; ‡Institut für Zellbiochemie, D-20246 Hamburg, Germany; and §Herzzentrum Leipzig GmbH, D-04289 Leipzig, Germany
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Hansjürgen Volkmer,
Hansjürgen Volkmer
*Max-Delbrück-Centrum für Molekulare Medizin, D-13122 Berlin, Germany; ‡Institut für Zellbiochemie, D-20246 Hamburg, Germany; and §Herzzentrum Leipzig GmbH, D-04289 Leipzig, Germany
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Fritz Buck,
Fritz Buck
*Max-Delbrück-Centrum für Molekulare Medizin, D-13122 Berlin, Germany; ‡Institut für Zellbiochemie, D-20246 Hamburg, Germany; and §Herzzentrum Leipzig GmbH, D-04289 Leipzig, Germany
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Albrecht Otto,
Albrecht Otto
*Max-Delbrück-Centrum für Molekulare Medizin, D-13122 Berlin, Germany; ‡Institut für Zellbiochemie, D-20246 Hamburg, Germany; and §Herzzentrum Leipzig GmbH, D-04289 Leipzig, Germany
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Attila Tárnok,
Attila Tárnok
*Max-Delbrück-Centrum für Molekulare Medizin, D-13122 Berlin, Germany; ‡Institut für Zellbiochemie, D-20246 Hamburg, Germany; and §Herzzentrum Leipzig GmbH, D-04289 Leipzig, Germany
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Fritz G. Rathjen
Fritz G. Rathjen
*Max-Delbrück-Centrum für Molekulare Medizin, D-13122 Berlin, Germany; ‡Institut für Zellbiochemie, D-20246 Hamburg, Germany; and §Herzzentrum Leipzig GmbH, D-04289 Leipzig, Germany
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Stefan Schumacher
*Max-Delbrück-Centrum für Molekulare Medizin, D-13122 Berlin, Germany; ‡Institut für Zellbiochemie, D-20246 Hamburg, Germany; and §Herzzentrum Leipzig GmbH, D-04289 Leipzig, Germany
Hansjürgen Volkmer
*Max-Delbrück-Centrum für Molekulare Medizin, D-13122 Berlin, Germany; ‡Institut für Zellbiochemie, D-20246 Hamburg, Germany; and §Herzzentrum Leipzig GmbH, D-04289 Leipzig, Germany
Fritz Buck
*Max-Delbrück-Centrum für Molekulare Medizin, D-13122 Berlin, Germany; ‡Institut für Zellbiochemie, D-20246 Hamburg, Germany; and §Herzzentrum Leipzig GmbH, D-04289 Leipzig, Germany
Albrecht Otto
*Max-Delbrück-Centrum für Molekulare Medizin, D-13122 Berlin, Germany; ‡Institut für Zellbiochemie, D-20246 Hamburg, Germany; and §Herzzentrum Leipzig GmbH, D-04289 Leipzig, Germany
Attila Tárnok
*Max-Delbrück-Centrum für Molekulare Medizin, D-13122 Berlin, Germany; ‡Institut für Zellbiochemie, D-20246 Hamburg, Germany; and §Herzzentrum Leipzig GmbH, D-04289 Leipzig, Germany
Siegfried Roth
Fritz G. Rathjen
*Max-Delbrück-Centrum für Molekulare Medizin, D-13122 Berlin, Germany; ‡Institut für Zellbiochemie, D-20246 Hamburg, Germany; and §Herzzentrum Leipzig GmbH, D-04289 Leipzig, Germany
Address all correspondence to Fritz G. Rathjen, Max-Delbrück-Centrum für Molekulare Medizin, Robert-Rössle-Str. 10, D-13122 Berlin, Germany.
Received:
August 22 1996
Revision Received:
December 11 1996
Online ISSN: 1540-8140
Print ISSN: 0021-9525
1997
J Cell Biol (1997) 136 (4): 895–906.
Article history
Received:
August 22 1996
Revision Received:
December 11 1996
Citation
Stefan Schumacher, Hansjürgen Volkmer, Fritz Buck, Albrecht Otto, Attila Tárnok, Siegfried Roth, Fritz G. Rathjen; Chicken Acidic Leucine-rich EGF-like Domain Containing Brain Protein (CALEB), a Neural Member of the EGF Family of Differentiation Factors, Is Implicated in Neurite Formation. J Cell Biol 24 February 1997; 136 (4): 895–906. doi: https://doi.org/10.1083/jcb.136.4.895
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