Issues
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ON THE COVER
Confocal image of tubular (t-) system trapped rhod-5N Ca2+-sensitive dye in a skinned skeletal muscle fiber (left ) and a schematic of the key Ca2+-handling players on the sarcoplasmic reticulum and t-system membranes that regulate t-system Ca2+ fluxes (right). Image © Meizoso-Huesca and Launikonis, 2020. See http://doi.org/10.1085/jgp.202012747. - PDF Icon PDF LinkTable of Contents
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Research News
Single molecule imaging reveals a slice of life
JGP study describes method to trace the real-time movements of individual membrane proteins in live tissue slices.
Reviews
Structural basis of cytoplasmic NaV1.5 and NaV1.4 regulation
In this review, Nathan et al. discuss recent structural insights into the regulation of the Na+ channels NaV1.5 and NaV1.4, facilitated by the combination of cryo-EM and x-ray crystallography data.
Articles
External Cd2+ and protons activate the hyperpolarization-gated K+ channel KAT1 at the voltage sensor
Zhou et al. show that the hyperpolarization-gated K+ channel KAT1 shares a homologous divalent cation/proton binding pocket in the external voltage sensor with depolarization-gated EAG family K+ channels. Divalent ions/protons inhibit EAG channels but potentiate activation of KAT1.
The Orai1 inhibitor BTP2 has multiple effects on Ca2+ handling in skeletal muscle
Meizoso-Huesca and Launikonis describe multiple effects of BTP2, an inhibitor of the Ca2+ channel Orai1, on Ca2+ handling in skeletal muscle, which include impairment of Ca2+ release from the sarcoplasmic reticulum and indirect effects on the activity of the ryanodine receptors.
Communications
Large transient capacitive currents in wild-type lysosomal Cl−/H+ antiporter ClC-7 and residual transport activity in the proton glutamate mutant E312A
Pusch and Zifarelli show that the lysosomal 2 Cl−/1 H+ antiporter ClC-7 mediates large, transient capacitive currents, which depend on external pH and internal Cl−. Unlike in other mammalian CLC antiporters, mutation of a conserved proton glutamate residue in ClC-7 does not abolish transport currents.
Methods and Approaches
A method for imaging single molecules at the plasma membrane of live cells within tissue slices
Mashanov et al. present a method to image and track individual fluorescently labeled molecules in live cardiac tissue slices using total internal reflection fluorescence (TIRF) microscopy. They show that the movement and interaction of G protein–coupled receptors can be observed in real time.
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