Issues

Cover picture: (Top) Schematic representation of classical signaling by M₁ muscarinic receptors (M₁R). (Bottom) FRET measurements of individual signaling steps (dots) compared to predictions of a kinetic model of the M₁R signaling cascade (numbered curves). (1) Agonist binding reported by intramolecular FRET. (2) G protein binding to the receptor, FRET from M1R-CFP/Gβ-YFP. (3) Nucleotide exchange, FRET from Gα_q-CFP/ Gβ-YFP. (4) Gα_q binding to PLC, FRET from Gα_q-CFP/ PLC-YFP (see article by Falkenburger et al., 81–97).