No evidence was found to support the idea that vaccine virus placed in the cisterna magna is capable of producing an acute disseminated encephalomyelitis with perivascular demyelination either in normal or in partially immune monkeys. A testicular extract (Reynals' factor) did not induce vaccine virus to cause an acute disseminated encephalomyelitis in monkeys. Repeated intramuscular injections of brain extracts and brain emulsions into eight monkeys were followed in two instances by an inflammatory reaction, accompanied by demyelination, in the central nervous system. The exact relation of the injections to the disease of the nervous system is not clear. The combined action of vaccine virus and an emulsion of fresh rabbit brain did not lead to the production of an acute disseminated encephalomyelitis in monkeys that had received repeated intramuscular injections of emulsions and alcohol-ether extracts of normal rabbit brains.
1. The virus of psittacosis is present in the nasal secretions, feces, blood, spleen, and liver of an infected parrot. 2. Parrots are susceptible to intraoral, intranasal, or intramuscular inoculations of the virus. 3. The most constant pathological changes produced by psittacosis in parrots occur in the spleen and liver. The lesions exhibited in the latter organ consist of areas of necrotic liver cells and damage to bile ducts. In no instance, in our experience, were lesions observed in a parrot's lungs comparable to those found in the lungs of men. 4. "Minute bodies" similar to those described by Levinthal and others were found in many, but not in all of the infected birds. 5. Parrots that have recovered from one attack of psittacosis exhibit an active immunity against reinfection.