1. Bacillus abortus Bang can be regularly recovered from guinea pigs inoculated with material containing the bacillus within 3 to 4 weeks.
2. The method is especially useful in recovering the organism from fetal membranes which, as a rule, are obtained after having come in contact with fecal matter, bedding, etc.
3. The spleen is the organ in which the bacteria are regularly present and in largest numbers. Cultures must be made from it to ensure success.