Effect of IRF8 on expression of BCL6 and AICDA. (A) Western blot analyses of IRF8 and α-tubulin expression in NFS-202 cells expressing a negative siRNA and clones expressing either suppressive siRNA #2 or #5. (B) qPCR analyses of gene transcripts in comparisons of NFS-202 cells with an inactive siRNA (closed bar) or with active siRNA #2 and #5 (open and shaded bars, respectively). Results obtained with cells with IRF8 siRNAs are normalized to the values for cells with the control siRNA. Results are representative of three experiments. (C) Luciferase reporter assays of Bcl6 and Aicda promoter sequences. HeLa cells were cotransfected with 800 ng pGL3-Bcl6 or pGL3-Aicda reporter vector, 0–300 ng pCDNA3.1-IRF8 or pCDNA3.1 empty expression vector, and 50 ng pRL-SV40 reporter vector. Luciferase activities were measured after 22 h, and transfection efficiency was normalized with values of Renilla luciferase activities. Results are representative of three experiments. Values in B and C indicate SD.
Effect of IRF8 on expression of BCL6 and AICDA. (A) Western blot analyses of IRF8 and α-tubulin expression in NFS-202 cells expressing a negative siRNA and clones expressing either suppressive siRNA #2 or #5. (B) qPCR analyses of gene transcripts in comparisons of NFS-202 cells with an inactive siRNA (closed bar) or with active siRNA #2 and #5 (open and shaded bars, respectively). Results obtained with cells with IRF8 siRNAs are normalized to the values for cells with the control siRNA. Results are representative of three experiments. (C) Luciferase reporter assays of Bcl6 and Aicda promoter sequences. HeLa cells were cotransfected with 800 ng pGL3-Bcl6 or pGL3-Aicda reporter vector, 0–300 ng pCDNA3.1-IRF8 or pCDNA3.1 empty expression vector, and 50 ng pRL-SV40 reporter vector. Luciferase activities were measured after 22 h, and transfection efficiency was normalized with values of Renilla luciferase activities. Results are representative of three experiments. Values in B and C indicate SD.
