Immunoglobulin (Ig)A provides the initial immune barrier to viruses at mucosal surfaces. Specific IgA interrupts viral replication in polarized epithelium during receptor-mediated transport, probably by binding to newly synthesized viral proteins. Here, we demonstrate by immunoelectron microscopy that specific IgA monoclonal antibodies (mAbs) accumulate within Sendai virus–infected polarized cell monolayers and colocalize with the hemagglutinin– neuraminidase (HN) viral protein in a novel intracellular structure. Neither IgG specific for HN nor irrelevant IgA mAbs colocalize with viral protein. Treatment of cultures with viral-specific IgA but not with viral-specific IgG or irrelevant IgA decreases viral titers. These observations provide definitive ultrastructural evidence of a subcellular compartment in which specific IgA and viral envelope proteins interact, further strengthening our hypothesis of intracellular neutralization of virus by specific IgA antibodies. Our results have important implications for intracellular protein trafficking, viral replication, and viral vaccine development.
Skip Nav Destination
Article navigation
5 October 1998
Article|
October 05 1998
Immunocytochemical Colocalization of Specific Immunoglobulin A with Sendai Virus Protein in Infected Polarized Epithelium
Hisashi Fujioka,
Hisashi Fujioka
From the *Institute of Pathology, the ‡Department of Pathology and the §Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106; and ‖The Institute of Medical Sciences, Tokai University, Boseidai, Isehara, Kanagawa 259-1193, Japan
Search for other works by this author on:
Steven N. Emancipator,
Steven N. Emancipator
From the *Institute of Pathology, the ‡Department of Pathology and the §Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106; and ‖The Institute of Medical Sciences, Tokai University, Boseidai, Isehara, Kanagawa 259-1193, Japan
Search for other works by this author on:
Masamichi Aikawa,
Masamichi Aikawa
From the *Institute of Pathology, the ‡Department of Pathology and the §Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106; and ‖The Institute of Medical Sciences, Tokai University, Boseidai, Isehara, Kanagawa 259-1193, Japan
Search for other works by this author on:
Dennis S. Huang,
Dennis S. Huang
From the *Institute of Pathology, the ‡Department of Pathology and the §Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106; and ‖The Institute of Medical Sciences, Tokai University, Boseidai, Isehara, Kanagawa 259-1193, Japan
Search for other works by this author on:
Frank Blatnik,
Frank Blatnik
From the *Institute of Pathology, the ‡Department of Pathology and the §Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106; and ‖The Institute of Medical Sciences, Tokai University, Boseidai, Isehara, Kanagawa 259-1193, Japan
Search for other works by this author on:
Tracy Karban,
Tracy Karban
From the *Institute of Pathology, the ‡Department of Pathology and the §Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106; and ‖The Institute of Medical Sciences, Tokai University, Boseidai, Isehara, Kanagawa 259-1193, Japan
Search for other works by this author on:
Kristin DeFife,
Kristin DeFife
From the *Institute of Pathology, the ‡Department of Pathology and the §Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106; and ‖The Institute of Medical Sciences, Tokai University, Boseidai, Isehara, Kanagawa 259-1193, Japan
Search for other works by this author on:
Mary B. Mazanec
Mary B. Mazanec
From the *Institute of Pathology, the ‡Department of Pathology and the §Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106; and ‖The Institute of Medical Sciences, Tokai University, Boseidai, Isehara, Kanagawa 259-1193, Japan
Search for other works by this author on:
Hisashi Fujioka
,
Steven N. Emancipator
,
Masamichi Aikawa
,
Dennis S. Huang
,
Frank Blatnik
,
Tracy Karban
,
Kristin DeFife
,
Mary B. Mazanec
From the *Institute of Pathology, the ‡Department of Pathology and the §Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106; and ‖The Institute of Medical Sciences, Tokai University, Boseidai, Isehara, Kanagawa 259-1193, Japan
Address correspondence to Mary B. Mazanec, Associate Professor of Medicine and Pathology, Department of Medicine, Case Western Reserve University, 10900 Euclid Ave., Cleveland, OH 44106. Phone: 216-844-3201; Fax: 216-844-3226; E-mail: [email protected]
Received:
January 21 1998
Revision Received:
June 18 1998
Online ISSN: 1540-9538
Print ISSN: 0022-1007
1998
J Exp Med (1998) 188 (7): 1223–1229.
Article history
Received:
January 21 1998
Revision Received:
June 18 1998
Citation
Hisashi Fujioka, Steven N. Emancipator, Masamichi Aikawa, Dennis S. Huang, Frank Blatnik, Tracy Karban, Kristin DeFife, Mary B. Mazanec; Immunocytochemical Colocalization of Specific Immunoglobulin A with Sendai Virus Protein in Infected Polarized Epithelium . J Exp Med 5 October 1998; 188 (7): 1223–1229. doi: https://doi.org/10.1084/jem.188.7.1223
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionSuggested Content
Email alerts
Advertisement
