CD40 is a member of the tumor necrosis factor (TNF) receptor family of cell surface proteins and was originally described as a B cell restricted antigen. Treatment of primary human monocytes with granulocyte/macrophage colony-stimulating factor (GM-CSF), interleukin 3 (IL-3), or interferon gamma (IFN-gamma) resulted in the induction of CD40 mRNA and enhancement of cell surface protein expression. CD40 was found to mediate monocyte adhesion to cells expressing recombinant CD40 ligand. CD40 ligand-transfected cells provided a potent costimulus for monocyte TNF-alpha and IL-6 production in the presence of GM-CSF, IL-3, or IFN-gamma, and enhanced IL-8 production stimulated by GM-CSF or IL-3. In addition, CD40 ligand-transfected cells acting in the absence of a costimulus induced monocytes to become tumoricidal against a human melanoma cell target. Collectively, these data indicate that CD40 ligand is pleiotropic with potent biological activity on monocytes.
CD40 expression by human monocytes: regulation by cytokines and activation of monocytes by the ligand for CD40.
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M R Alderson, R J Armitage, T W Tough, L Strockbine, W C Fanslow, M K Spriggs; CD40 expression by human monocytes: regulation by cytokines and activation of monocytes by the ligand for CD40.. J Exp Med 1 August 1993; 178 (2): 669–674. doi: https://doi.org/10.1084/jem.178.2.669
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