T lymphocytes from guinea pigs immunized with 2,4-dinitrophenyl (DNP) derivatives of mycobacteria respond to a variety of DNP conjugates. Preincubation of such cells with a given DNP conjugate under conditions which lead to the inactivation of responding cells causes a loss of the response to that conjugate, but has little effect on the response to DNP coupled to unrelated carriers. Thus, the responses of such cells to a variety of DNP conjugates can best be explained by the presence of a mixture of highly specific cells each responding to a different antigenic dterminant rather than by the presence of T cells with specificity limited to the hapten itself. Furthermore, the activity of T cells from DNP-mycobacteria-primed donors could not be blocked by a variety of nonstimulatory DNP conjugates. This suggests that while such T cells clearly recognize DNP with great precision, the receptor does not contain a very high affinity site for the hapten. A possible model for such a T-cell receptor is discussed.
The specificity of cellular immune responses in guinea pigs. III. The precision of antigen recognition by T lymphocytes.
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C A Janeway, W E Paul; The specificity of cellular immune responses in guinea pigs. III. The precision of antigen recognition by T lymphocytes.. J Exp Med 1 December 1976; 144 (6): 1641–1656. doi: https://doi.org/10.1084/jem.144.6.1641
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