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Vol. 221, No. 10 | https://doi.org/10.1084/jem.20231143 | September 5, 2024

The authors regret that in Fig. S3 N of their original article, the first three dot plot images (CD19, GL7, and IgD) in the third row of VavPBcl2;SgLacZ were mistakenly duplicated from the third row of VavPBcl2;SgSETD1B. The original and corrected figures are shown here. The error affects only the images; the quantification and statistics were derived from the raw data and are unchanged, and the correction does not alter the conclusions of the study. The HTML and PDF versions of this article have been corrected. The error remains only in PDFs downloaded before October 12, 2026.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

Data & Figures

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Figure. Refer to the image caption for details. Panel A shows a schematic of the experimental setup with mice, HPCs, and irradiated recipients. Panel B is a Kaplan-Meier survival curve showing lymphoma-free percentages over time for two groups, sgLacZ and sgSetd1b (sg2). Panel C displays gel electrophoresis results for Seltd1b sg1 and sg2. Panel D shows images of spleens from different experimental groups. Panels E and F are dot plots showing the percentage of fluorescent positive cells in HSCs and spleens for sgSetd1b and sgKmt2d groups. Panel G is a bar graph showing the percentage of subpopulations in HSCs and spleens for different experimental groups. Panel H contains histological images of spleen sections stained for various markers. Panel I shows histological images of spleen sections stained for different markers comparing sgLacZ and sgSetd1b groups. Panel J displays a gel electrophoresis result for the IgL locus. Panel K is a table summarizing various data points. Panel L shows histological images of liver sections stained for different markers. Panel M contains histological images of kidney sections stained for different markers. The corrected figure highlights the changes made to the original figure, specifically in the dot plot images.
A multi-panel image showing experimental data on mice and graphs. Panel A shows a schematic of the experimental setup with mice, HPCs, and irradiated recipients. Panel B is a Kaplan-Meier survival curve showing lymphoma-free percentages over time for two groups, sgLacZ and sgSetd1b (sg2). Panel C displays gel electrophoresis results for Seltd1b sg1 and sg2. Panel D shows images of spleens from different experimental groups. Panels E and F are dot plots showing the percentage of fluorescent positive cells in HSCs and spleens for sgSetd1b and sgKmt2d groups. Panel G is a bar graph showing the percentage of subpopulations in HSCs and spleens for different experimental groups. Panel H contains histological images of spleen sections stained for various markers. Panel I shows histological images of spleen sections stained for different markers comparing sgLacZ and sgSetd1b groups. Panel J displays a gel electrophoresis result for the IgL locus. Panel K is a table summarizing various data points. Panel L shows histological images of liver sections stained for different markers. Panel M contains histological images of kidney sections stained for different markers. The corrected figure highlights the changes made to the original figure, specifically in the dot plot images.
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Figure S3.
Figure S3. Refer to the image caption for details. Panel A shows a schematic of the experimental setup with mice, HPCs, and irradiated recipients. Panel B is a Kaplan-Meier survival curve showing lymphoma-free percentages over time for two groups, sgLacZ and sgSetd1b (sg2). Panel C displays gel electrophoresis results for Seltd1b sg1 and sg2. Panel D shows images of spleens from different experimental groups. Panels E and F are dot plots showing the percentage of fluorescent positive cells in HSCs and spleens for sgSetd1b and sgKmt2d groups. Panel G is a bar graph showing the percentage of subpopulations in HSCs and spleens for different experimental groups. Panel H contains histological images of spleen sections stained for various markers. Panel I shows histological images of spleen sections stained for different markers comparing sgLacZ and sgSetd1b groups. Panel J displays a gel electrophoresis result for the IgL locus. Panel K is a table summarizing various data points. Panel L shows histological images of liver sections stained for different markers. Panel M contains histological images of kidney sections stained for different markers. The corrected figure highlights the changes made to the original figure, specifically in the dot plot images.

Combined loss of SETD1B and KMT2D leads to a more aggressive disease. (A) Illustration of FL adoptive transfer model based on the VavP-BCL2 transgenic and retroviral transduction of HPCs followed by reconstitution in lethally irradiated, syngeneic, female mice. (B) Survival curves for mice harboring sgRNA targeting LacZ (sgLacZ) and mice carrying sgRNA targeting Setd1b (sgSetd1b) (***P = 0.005). P value was calculated using log-rank (Mantel–Cox) test. (C) Detection of genomic editing of Setd1b sgRNAs by T7 endonuclease I (T7E1) assay. The target fragments were amplified by PCR from genomic DNA from mouse tumors. Arrows indicate the digested fragments by T7EI. Data representative of at least two independent experiments are shown. (D) Representative spleens taken from each mouse group. Spleens from VavP-Bcl2; sgSetd1b (n = 27), VavP-Bcl2; sgKmt2d (n = 8), and VavP-Bcl2; sgkmt2d+sgSetd1b (n = 6) were enlarged compared with the control group, VavP-Bcl2; sgLacZ (n = 22). (E and F) Dot plot graph showing the percentage of positive transduced VavP-Bcl2 HSCs (prior to injection into mice) and splenic lymphoma cells (after injection and disease), indicating cells harboring (E) sgSetd1b-RFP (sgSetd1bRFP) or (F) sgKmt2d-GFP (sgKmt2dGFP) plasmids, (n = 8). (G) Stacked bar graph showing RFP and GFP-positive transduced VavP-Bcl2 HSCs (prior to injection into mice) and splenic lymphoma cells (after injection and disease), indicating cells harboring sgSetd1bRFP, sgKmt2dGFP plasmids or both sgSetd1bRFP and sgKmt2dGFP (RFP; GFP) (n = 3). (H) Additional immunohistochemistry serial micrographs of VavP-Bcl2 spleen tissues extracted from recipient mice upon sacrifice. VavP-Bcl2; sgLacZ, VavP-Bcl2; sgSetd1b, VavP-Bcl2; sgKmt2d, and VavP-Bcl2; sgSetd1b+Kmt2d were stained with H&E, B220, Ki67, PNA, TUNEL, and BCL6 of the liver tissues extracted from recipient mice upon sacrifice (n = 3 per group). Insets are 3× magnified. Scale bar: 200 μm, 500 μm. (I) Representative histological micrographs of VavP-Bcl2; sgLacZ versus VavP-Bcl2; sgSetd1b, stained with H&E, B220, PNA, and Ki67 (n = 3 per group). Scale bar: 1 mm. (J) DNA gel representing Ig heavy chain variable region (IgVH) rearrangements in B220+ cells isolated from VavP-Bcl2;sgLacZ and VavP-Bcl2;sgSetd1b spleens. Data representative of two independent experiments are shown. (K) Table summarizing the results of SHM analysis in DNA collected from VavP-Bcl2;sgSetd1b to VavP-Bcl2;sgLacZ tumors. (L and M) H&E and B220 staining of kidney (M) and liver (L) tissues extracted from recipient mice upon sacrifice. n = 3 per group. (N) Flow cytometry analysis of the cellular composition of splenic tumor cells from three mice in each genotype, comparing VavP-Bcl2;sgSetd1b to VavP-Bcl2;sgLacZ. Source data are available for this figure: SourceData FS3.

Figure S3.
A multi-panel image showing experimental data on mice and graphs. Panel A shows a schematic of the experimental setup with mice, HPCs, and irradiated recipients. Panel B is a Kaplan-Meier survival curve showing lymphoma-free percentages over time for two groups, sgLacZ and sgSetd1b (sg2). Panel C displays gel electrophoresis results for Seltd1b sg1 and sg2. Panel D shows images of spleens from different experimental groups. Panels E and F are dot plots showing the percentage of fluorescent positive cells in HSCs and spleens for sgSetd1b and sgKmt2d groups. Panel G is a bar graph showing the percentage of subpopulations in HSCs and spleens for different experimental groups. Panel H contains histological images of spleen sections stained for various markers. Panel I shows histological images of spleen sections stained for different markers comparing sgLacZ and sgSetd1b groups. Panel J displays a gel electrophoresis result for the IgL locus. Panel K is a table summarizing various data points. Panel L shows histological images of liver sections stained for different markers. Panel M contains histological images of kidney sections stained for different markers. The corrected figure highlights the changes made to the original figure, specifically in the dot plot images.

Combined loss of SETD1B and KMT2D leads to a more aggressive disease. (A) Illustration of FL adoptive transfer model based on the VavP-BCL2 transgenic and retroviral transduction of HPCs followed by reconstitution in lethally irradiated, syngeneic, female mice. (B) Survival curves for mice harboring sgRNA targeting LacZ (sgLacZ) and mice carrying sgRNA targeting Setd1b (sgSetd1b) (***P = 0.005). P value was calculated using log-rank (Mantel–Cox) test. (C) Detection of genomic editing of Setd1b sgRNAs by T7 endonuclease I (T7E1) assay. The target fragments were amplified by PCR from genomic DNA from mouse tumors. Arrows indicate the digested fragments by T7EI. Data representative of at least two independent experiments are shown. (D) Representative spleens taken from each mouse group. Spleens from VavP-Bcl2; sgSetd1b (n = 27), VavP-Bcl2; sgKmt2d (n = 8), and VavP-Bcl2; sgkmt2d+sgSetd1b (n = 6) were enlarged compared with the control group, VavP-Bcl2; sgLacZ (n = 22). (E and F) Dot plot graph showing the percentage of positive transduced VavP-Bcl2 HSCs (prior to injection into mice) and splenic lymphoma cells (after injection and disease), indicating cells harboring (E) sgSetd1b-RFP (sgSetd1bRFP) or (F) sgKmt2d-GFP (sgKmt2dGFP) plasmids, (n = 8). (G) Stacked bar graph showing RFP and GFP-positive transduced VavP-Bcl2 HSCs (prior to injection into mice) and splenic lymphoma cells (after injection and disease), indicating cells harboring sgSetd1bRFP, sgKmt2dGFP plasmids or both sgSetd1bRFP and sgKmt2dGFP (RFP; GFP) (n = 3). (H) Additional immunohistochemistry serial micrographs of VavP-Bcl2 spleen tissues extracted from recipient mice upon sacrifice. VavP-Bcl2; sgLacZ, VavP-Bcl2; sgSetd1b, VavP-Bcl2; sgKmt2d, and VavP-Bcl2; sgSetd1b+Kmt2d were stained with H&E, B220, Ki67, PNA, TUNEL, and BCL6 of the liver tissues extracted from recipient mice upon sacrifice (n = 3 per group). Insets are 3× magnified. Scale bar: 200 μm, 500 μm. (I) Representative histological micrographs of VavP-Bcl2; sgLacZ versus VavP-Bcl2; sgSetd1b, stained with H&E, B220, PNA, and Ki67 (n = 3 per group). Scale bar: 1 mm. (J) DNA gel representing Ig heavy chain variable region (IgVH) rearrangements in B220+ cells isolated from VavP-Bcl2;sgLacZ and VavP-Bcl2;sgSetd1b spleens. Data representative of two independent experiments are shown. (K) Table summarizing the results of SHM analysis in DNA collected from VavP-Bcl2;sgSetd1b to VavP-Bcl2;sgLacZ tumors. (L and M) H&E and B220 staining of kidney (M) and liver (L) tissues extracted from recipient mice upon sacrifice. n = 3 per group. (N) Flow cytometry analysis of the cellular composition of splenic tumor cells from three mice in each genotype, comparing VavP-Bcl2;sgSetd1b to VavP-Bcl2;sgLacZ. Source data are available for this figure: SourceData FS3.

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